the structure of a capillary network
Báo cáo khoa học: Unravelling the functional interaction structure of a cellular network from temporal slope information of experimental data docx
Ngày tải lên :
07/03/2014, 21:20
... however,
only applicable to the case when for each node there are
as many parameters as the number of overall network
nodes and these parameters do not directly affect the
corresponding node. The fundamental ... experimental data containing (partially) cor-
rect dynamic pattern changes then we can infer the
(partial) interaction structure of the underlying cellular
network by integrating the analysis results ... have
proposed a general method for identification of a cellu-
lar network structure based on stationary experimental
data, which is applicable to a network of generalized
modules under the assumption that...
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Tài liệu Báo cáo " The meaning and structure of a science fiction story: a sysyemic functional analysis " doc
Ngày tải lên :
12/02/2014, 20:20
...
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative ...
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
declarative
imperative
declarative
declarative
ability/neg. ...
anaphoric
anaphoric
anaphoric
exophoric
anaphoric
exophoric
anaphoric
anaphoric
anaphoric
anaphoric
exophoric
anaphoric
anaphoric
anaphoric
anaphoric
exophoric
anaphoric
anaphoric...
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Tài liệu Báo cáo khoa học: Crystal structure of Klebsiella sp. ASR1 phytase suggests substrate binding to a preformed active site that meets the requirements of a plant rhizosphere enzyme doc
Ngày tải lên :
16/02/2014, 09:20
... a ⁄ b domain than in the a domain [20].
This is also reflected in the rigidity of the protein. The
atomic displacement factors of the PhyK structure are
smaller for the a ⁄ b domain than for the ... coli phytase, glucose-
1-phosphatase and human prostatic-acid phosphatase. The polypeptide
chain is organized into an a and an a ⁄ b domain, and the active site is
located in a positively charged ... substrate-free
AppA the C
a
atoms are 2.41 A
˚
apart, whereas for the
substrate-free PhyK and the substrate-loaded AppA
the averaged distance is only 1.87 A
˚
.
Distinct conformational changes were observed...
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Tài liệu Báo cáo khoa học: Evidence that the assembly of the yeast cytochrome bc1 complex involves the formation of a large core structure in the inner mitochondrial membrane pdf
Ngày tải lên :
18/02/2014, 08:20
... because of the absence of the catalytic
subunit ISP (Table 1). Figure 3A shows that a band of
approximately 500 kDa was also found in this mutant
strain when the mitochondrial membranes were ana-
lyzed ... dimerization of the bc
1
complex at
this stage (i.e. just after the addition of ISP and Qcr10p).
On the other hand, in the transition from the 500 kDa
band to the 670 kDa band, a structural rearrangement
of ... were of
analytical grade.
Yeast strains and growth media
The genotypes and sources of the S. cerevisiae strains are
described in Table 2. The ISP deletion strain was
prepared in accordance with the...
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Tài liệu Báo cáo khoa học: Insights into the structure of plant a-type phospholipase D Susanne Stumpe, Stephan Konig and Renate Ulbrich-Hofmann ¨ ppt
Ngày tải lên :
19/02/2014, 02:20
... that the calcium-binding event
of lower affinity is attributable to the catalytic site of
PLDa2, as found for PLDb from A. thaliana [39].
The decrease in PLD activity at concentrations
above the ... Journal 274 (2007) 2630–2640 ª 2007 The Authors Journal compilation ª 2007 FEBS 2633
from the ratio of the forward scattering intensity of the
sample and that of the molecular mass standard BSA. The
ab ... 2B).
Reconstruction of the overall shape of the PLDa2
from the X-ray scattering data was achieved by the
ab initio modeling program dammin [24]. The superpo-
sition of the 10 calculated models (Fig. 3) shows a
longish...
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Tài liệu Báo cáo khoa học: ˚ The 1.8 A crystal structure of a proteinase K-like enzyme from a psychrotroph Serratia species docx
Ngày tải lên :
19/02/2014, 07:20
... SPRK also has an aspar-
tic acid residue at position at 200, and with the same
conformation as in the two other structures, but
Asp200 forms a salt bridge to Lys253 (Ala253 and
Asn250 in PRK and ... [18]. The biophysical characteri-
zation of SPRK did not reveal the classical cold adap-
ted features [19], but still initial comparative studies
showed that the catalytic turnover was at least ... reported.
Results and discussion
Crystallization, data collection and refinement
Crystals of the catalytic domain of the Serratia prote-
inase K (SPRK) grew within a few days to thin hexa-
gonal plates growing...
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Tài liệu Báo cáo khoa học: "The Utility of a Graphical Representation of Discourse Structure in Spoken Dialogue Systems" ppt
Ngày tải lên :
20/02/2014, 12:20
... system. Also, their underlying
task (air travel domain) is much simpler than our
tutoring task. In addition, the SIH is not always
available and users have to activate it manually.
Other visual ... hints/de-
scribes the answer, each discourse segment has an
additional purpose annotation that is displayed
when the segment is part of the visible horizon.
Auto-collapse. To reduce the amount of infor-
mation ... also have an
equivalent in a new domain (e.g. displaying the
recognized user answer can be the equivalent of
the correct answers). Other NM usages can also be
imagined: e.g. reducing the length...
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Báo cáo khoa học: Structure of Streptococcus agalactiae serine⁄threonine phosphatase The subdomain conformation is coupled to the binding of a third metal ion pptx
Ngày tải lên :
07/03/2014, 09:20
... Hospital and Regional Medical Center, Seattle, WA, USA
Protein phosphatases are primarily classified on the
basis of the type of the amino acid they dephosphory-
late, serine ⁄ threonine phosphatases ... fused to an EF-hand motif, and human STP
(HsSTP), which has an additional 8 kDa a- helical
domain at the C-terminus [3,4].
The sizes of the catalytic domains of both PPP and
PPM families are well ... kinase and adenylosuccinate syn-
thase, but not in S. agalactiae CovR ⁄ CsrR. The motif
is located at the surface of the SaPPase (Rantanen,
unpublished), and superposition of the prolines allows
superposition...
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Báo cáo khoa học: Structure of the complex of a yeast glucoamylase with acarbose reveals the presence of a raw starch binding site on the catalytic domain doc
Ngày tải lên :
07/03/2014, 12:20
... GLU H44 7A forward (5¢- GCAAGTCATTT
TGGATGCTATTAATGATGATGGCTC-3¢), GLU H44 7A
reverse (5¢- GAGCCATCATCATTAATAGCATCCAAAA
TGACTTGC-3¢); GLU T46 2A forward (5¢- GAACAACTT
AACAGATATGCCGGTTATT CCACCGGT ... as a
template. The following oligonucleotides were used: GLU
R1 5A forward (5¢-ATTCAAACTATAAAGTTGACGCAA
CTGACTTGGAAACCTTC-3¢), GLU R1 5A reverse (5¢-
GAAGGTTTCCAAGTCAGTTGCGTCAACTTTATAGTT
TGAAT-3¢); ... GLU
T46 2A reverse (5¢- GGCACCGGTGGAATAACCGGCA
TATCTGTTAAGTTGTTC-3¢); GLU H44 7A, D45 0A for-
ward (5¢-GCAAGTCATTTTGGATGCTATTAATGCTG
ATGGCTCCTTGAATGAAC-3¢), GLU H44 7A, D45 0A
Fig. 7. Adsorption to raw...
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Inside the Structure of Defined Contribution/401(k) Plan Fees: A Study Assessing the Mechanics of the ‘All-In’ Fee pot
Ngày tải lên :
07/03/2014, 13:20
... In the report, Exhibit 3 plots the impact of average account balance and number of plan participants on the ‘all-in’ fee for a variety of combinations of average account balance and number of ... assets to arrive at the ‘all-in’ fee as a percentage
of plan assets. Also, each plan’s total dollar fee amount
was divided by total participants in the plan to arrive at
the ‘all-in’ fee as ... further analysis
found that a core specification that allowed average
account balances and number of participants (both in logs)
to affect fees as a percentage of assets each had separate
and...
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Báo cáo khoa học: The oxidative effect of bacterial lipopolysaccharide on native and cross-linked human hemoglobin as a function of the structure of the lipopolysaccharide A comparison of the effects of smooth and rough lipopolysaccharide ppt
Ngày tải lên :
08/03/2014, 10:20
... to an increase in the rate
of the initial fast phase, i.e. oxidation of the a chains. The
rates of oxidation are reduced in the presence of chelators of
heavy metal cations in most cases. An ... 3A.
The rate of auto-oxidation of cross-linked Hb was greater
than that of Hb A
0,
both in the presence and absence of
EDTA, as has been observed previously [17]. In addition, the
rates of auto-oxidation ... binding of the Hb tetramer to the LPS
molecule makes the heme cavity of the a chains more
accessible to a water molecule which can then accelerate the
displacement of the protonated superoxide anion,...
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Báo cáo Y học: Electrostatic properties of the structure of the docking and dimerization domain of protein kinase A IIa doc
Ngày tải lên :
08/03/2014, 22:20
... demonstrate the presence of positive charge.
Figure 4I shows the bottom face (same as in Fig. 4C) of a
van der Waals model of the best structure of RIIa D/D. The
central part of this face appears ... two basic sites, the N-terminal
amide and His2. Aspartic acids Asp27 and Asp30 show
lower mean pK
a
values than their model pK
a
values. This
may be a general property of Asp residues, as it has ... To
examine the electrostatic character of the structure of RIIa
D/D we have predicted mean apparent pK
a
values, based on
Poisson–Boltzmann electrostatic calculations, using an
ensemble of calculated...
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Báo cáo khoa học: Structure of a trypanosomatid mitochondrial cytochrome c with heme attached via only one thioether bond and implications for the substrate recognition requirements of heme lyase potx
Ngày tải lên :
23/03/2014, 04:21
... compilation ª 2009 FEBS 2831
of the alanine of the AXXCH motif (Ala25) (in
green) and the unsaturated vinyl group of the heme
(cyan) are separated by 3.41 A
˚
(as compared with a
typical thioether ... 72% similarity. The structures
are remarkably similar overall, and in the details of
the heme attachment and heme position. The rmsd
between the structure of C. fasciculata cytochrome c
and S. ... Crystallographic data were collected at
beamline IO2 at Diamond Light Source, UK, and we
acknowledge the support of T. Sorensen at Diamond
and A. Labrador at the ESRF, France.
References
1 Barker...
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Báo cáo khoa học: The crystal structure of a xyloglucan-specific endo-b-1,4glucanase from Geotrichum sp. M128 xyloglucanase reveals a key amino acid residue for substrate specificity potx
Ngày tải lên :
23/03/2014, 05:22
... Reykjavik, Iceland), and were refined by varying
the pH of the buffer and the concentration of the precipi-
tant. Prior to data collection, a crystal was transferred into
Paratone-N (Hampton Research) ... used as
a search model. The cns program [19] was used for
refinement against the 20–2.5 A
˚
intensity data. A ran-
domly selected portion of the diffraction data (5.0%)
was used to calculate the ... kinetic parameters
The kinetic parameters were determined at various concen-
trations of the substrate, tamarind seed xyloglucan (Dainip-
pon Sumitomo Pharma, Osaka, Japan), in 50 mm sodium
acetate...
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