... appears to be an improved network of inter-actions, which is reflected in the increased frequency of totalnumber of atoms, charged amino acids and hydrogenbonds. Another feature appears to be ... data sets. Iterative cycles of refinement and manual fitting were carried out using programsCNS[24] andO[25]. To monitor the progress of the refinement, a total of 10% of the reflections were setaside ... Arg58 (Oc1andOc2 atoms of aspartic acid and Ng1andNg2atomsofarginine). In CTX, Arg161 makes a salt bridge with Asp57.Hence, the a-helix region of these two enzymes is likely to bestabilized...