... [pPBCab] containing the penDE gene of P chrysogenum without and with induction (note formation of the 29-kDa and 11-kDa subunits in lane 2); lanes and 4, E coli [pULCTab] containing the penDE gene ... 15-min pulse of [35S]methionine/[35S]cysteine Lanes 3, 6, 9, 12 and 15 were not induced In the autoradiography, note the formation of a 40-kDa labelled protein in lanes and (containing the ab constructions) ... induction and lanes and with 0.5 mM IPTG) Lanes 3, 4, 10 and 11, E coli [pULCTab], contains the gene penDE from A nidulans (lanes and 10 without IPTG induction and lanes and 11 with 0.5 mM IPTG) Lane...