b anti brdu antibody detection of dna recombination and repair
Development of gold nanoparticle DNA nanostructure assembly for detection of DNA, RNA and protein biomarkers
Ngày tải lên :
09/09/2015, 11:16
... Dual tier detection of let7a miRNA using a PG-AuNP-MB system (5PG:5MB, at 300mM K+) Blue - fluorescence intensity; brown - size of system xi LIST OF TABLES Table 3.1 Sequence of probe and target ... different probes are cross-hybridized with the respective let7 targets Table 5.1 Sequence of A, B and C ssDNA A and B is complementary to linker AB, while A and C bind to linker AC Table 5.2 Average ... organization of DNA into assemblies was also the basis for DNA- based nanomachines, in which the transition of the B to Z conformation was accompanied by the displacement of the DNA strands, as shown by...
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Báo cáo khoa học: Detection of nucleolar organizer and mitochondrial DNA insertion regions based on the isochore map of Arabidopsis thaliana ppt
Ngày tải lên :
30/03/2014, 20:20
... Chromosome I: b II: mtDNA insertion in region c III: e IV: a, c I: b, c, d, e II: b, mtDNA insertion in region c III: e IV: a, c V: a, b, c I: a, b, c, d, e II: b, d, e, mtDNA insertion III: a, b, c, ... slope of the z¢ curve within the region Dn Both quantities of Dzn¢ and Dn can be FEBS Journal 272 (2005) 3328–3336 ª 2005 FEBS Acknowledgements We thank Prof Chun-Ting Zhang for invaluable assistance ... 1, and they are located in 0–230 kb of chromosomes II and 0–350 kb of chromosomes IV, respectively The similar genomic organization of chromosomes I, III and V makes their overall GC distribution...
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in situ detection of dna damage, methods and protocols
Ngày tải lên :
11/04/2014, 09:47
... Incubate cells in 0.2 µg/mL DAPI, and rinse once in PBS To label fixed samples using both TUNEL and immunofluorescence staining for an antigen of interest, the antibody incubations first, then block ... followed by autoradiography 3.3.3 Labeling of 5'-OH Ends of DNA Prepare a reaction tube by adding µL of 10× T4 kinase reaction buffer, µL of sample from step of Subheading 3.3.1., µL (50 Units) of ... it should be handled with gloves This kit supplies equilibration buffer, reaction buffer, TdT enzyme, stop/wash buffer and anti- digoxigenin antibody conjugated to peroxidase Instead of anti- digoxigenin...
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báo cáo khoa học: "Detection of DNA mismatch repair proteins in fresh human blood lymphocytes - towards a novel method for hereditary non-polyposis colorectal cancer (Lynch syndrome) screening" doc
Ngày tải lên :
10/08/2014, 10:21
... Anti- MSH2(Ab-2) mouse mAb(FE11) NA27 EMD Calbiochem, Gibbstown, NJ MLH1 Anti- MSH2(Ab-1)mouse mAb(GB12) 554073 NA26T BD Pharmingen, San Diego, CA Calbiochem, San Diego, CA Anti- MLH1(Ab-1)mouse mAb(14) ... F Density of the bands of interest on a western blot was determined by scanning of the x-ray film and highlighting the band area using a BioRad Gel 2000 documentation system and its software The ... of Table Commercially available monoclonal and polyclonal antibodies used for detection of MLH1 and MSH2 proteins on western blots No Names Catalog Number Company Monoclonal Antibodies Anti- MSH2(Ab-2)...
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báo cáo khoa học: " Detection of DNA fusion junctions for BCR-ABL translocations by Anchored ChromPET" pot
Ngày tải lên :
11/08/2014, 12:21
... KU812 cells and patient 1, some of the DNA at the BCR locus seems to be duplicated, so that the BCR breakpoint in the BCR-ABL fusion is downstream of the BCR breakpoint in the ABL-BCR fusion (Figures ... 149,445-149,446 119 BCR-ABL1 109,834-109,835 102,524-102,525 0 ABL1-BCR 109,702-109,867 ABL1 ABL1-BCR Patient M-BCR ABL1-BCR Sample ABL1 109,869-109,870 102,526-102,527 0 a 23,822 Predicted and actual breakpoints ... peripheral blood to detect the juxtaposition of BCR and ABL signals created by a translocation In this case, about normal KU812 ABL1-BCR normal KU812 normal KU812 BCR-ABL1 K562 (a) 100 bp ladder Shibata...
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Xpert MTB/RIF test for detection of pulmonary tuberculosis and rifampicin resistance (Protocol) pptx
Ngày tải lên :
06/03/2014, 04:20
... accuracy using definite TB and probable-TB patients combined for sensitivity calculations, and non-TB and probable TB patients combined for specificity calculations For review question B, we will sort ... question B will be adults suspected of having pulmonary TB or MDR-TB, from all settings and all countries Patients suspected of having MDR-TB may include patients with a history of TB, patients on TB ... point of treatment in high-TB burden countries are urgently needed Target condition being diagnosed TB is an airborne disease caused by the bacterium, M tuberculosis, and is spread primarily by...
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Báo cáo khoa học: Degradation of chitosans with chitinase B from Serratia marcescens Production of chito-oligosaccharides and insight into enzyme processivity docx
Ngày tải lên :
07/03/2014, 16:20
... frequencies and block lengths of each chitosan are compared with the calculated diad frequencies and block lengths of a chitosan with a random (Bernoullian) distribution of A- and D-units (numbers given ... started by adding 10 lg of ChiB and the reactions were stopped after week as described above FEBS Journal 272 (2005) 538549 ê 2005 FEBS A Sứrbotten et al Degradation of chitosans with ChiB References ... site of ChiB has dened subsites from )3 to +3 [16,20], and the substrate-binding cleft is tunnel-like, permitting tight interaction with the polymeric substrate The aglyconside of the substrate-binding...
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Báo cáo khoa học: " Improved Automatic Detection of Zero Subjects and Impersonal Constructions in Spanish" docx
Ngày tải lên :
31/03/2014, 21:20
... precision of 74% and recall u of 60%, and K-nearest neighbors in TiMBL: both in (Evans, 2001) with precision of 73% and recall of 69%, and in (Boyd et al., 2005) with precision of 82% and recall of ... referential] Of these annotated verbs, 71% have an explicit subject, 26% have a zero subject and 3% belong to an impersonal construction (see Table 2) Number of instances Explicit subjects Zero subjects ... the preceding verb in the sentence and also with the main verb of the sentence.3 7-9 NHPREV, NHTOT, INF: the candidates for the subject of the clause are represented by the number of noun phrases...
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Báo cáo sinh học: "Concomitant detection of IFNa signature and activated monocyte/dendritic cell precursors in the peripheral blood of IFNa-treated subjects at early times after repeated local cytokine treatments" doc
Ngày tải lên :
18/06/2014, 19:20
... clustering of all samples collected before (T0, T1m, blue bar) or after (T0+24, T1m+24, red bar) the first and the second cycle of administration of HBV vaccine in combination with placebo (black bar, ... Transcribed locus MTMR1 OTOF LGALS3BP CCR1 ISGF3G GLUL Transcribed locus CXCL10 CD38 RSAD2 EIF2AK2 Transcribed locus HBZ HBE1 HBBP1 Pre Tx Post Tx Group C (HBV Vaccine + 3MU LE-IFN ) Group B (HBV ... evaluate and compare the modulations of PBMC global gene expression profiles induced by IFNa in melanoma and normal donors The effects of the administration of different doses of IFNa, as well as of...
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Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript" ppt
Ngày tải lên :
09/08/2014, 20:20
... the bait whose sequence contains the breakpoint enable this recovery and enrichment This may be possible because the cDNA library inserts are 290 to 390 bp, which is larger than the 170-base baits ... fraction of 467 genes at or above that threshold is plotted (y-axis) for before hybrid selection (blue) and after hybrid selection (red) Table Analysis of Illumina sequence in cDNA hybrid selection ... 1,085,748 bases in the coding sequence of targeted genes, 297,693 bases exhibited LOD greater than before hybrid selection, and 724,211 bases exhibited LOD greater than after hybrid selection Bases...
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báo cáo khoa học: "C60-Fullerenes: detection of intracellular photoluminescence and lack of cytotoxic effects" pdf
Ngày tải lên :
11/08/2014, 00:22
... presence of a heterogenous mixture of variably sized clusters Furthermore, following evaporation of methanol, the majority of fullerene clusters not reaggregate, and have a range of sizes of tens of ... 200 nm) may not be detectable by this technique Recent reports indicate the ability to detect fluorescence of Page of 11 (page number not for citation purposes) Journal of Nanobiotechnology 2006, ... solution was obtained and all C60 had been dissolved as determined by observation Following sonication in tolu- Page of 11 (page number not for citation purposes) Journal of Nanobiotechnology...
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Báo cáo y học: " Anti-HIV-1 activity of salivary MUC5B and MUC7 mucins from HIV patients with different CD4 counts" doc
Ngày tải lên :
12/08/2014, 01:22
... dilutions of goat anti- MUC 5B (A) and goat anti- MUC7 (B) polyclonal antibodies at concentrations between μg/ml and 0.25 μg/ml (goat anti- MUC 5B) and 12 μg/ml and 0.375 μg/ml (goat anti- MUC7) Antibody binding ... rabbit anti- goat HRPO linked secondary antibody and visualized with TMB/H2O2 substrate Absorbance values were read at 405 nm in a Titertek ELISA reader Each point is the average absorbance of ... Polyclonal rabbit antiMUC 5B (LUM 5B- 2) and goat anti- rabbit horse radish peroxidise (HRPO) linked secondary antibodies were kindly provided by Sara Kirkham (Manchester, UK) Polyclonal goat anti- MUC 5B (sc-23024),...
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báo cáo khoa học: " Dynamics of DNA methylation and Histone H4 acetylation during floral bud differentiation in azalea" doc
Ngày tải lên :
12/08/2014, 03:21
... representative membrane) Average values of abundance index of AcH4 (± SE) Lane 1: tubulin and AcH4 of bud in phase A; Lane 2: tubulin and AcH4 of bud in phase B; Lane 3: tubulin and AcH4 of bud in phase ... DIC of bud in phase B (20×) (n) DAPI (blue signals) superposition and 5-mdC (green signals) of bud in phase B (20×) (o) DAPI labelling of nuclei of bud in phase B (20×) (p) 5-mdC labelling of bud ... DIC of bud in phase B (10×) (j) DAPI (blue signals) superposition and AcH4 (green signals) of bud in phase B (10×) (k) DAPI labelling of nuclei of bud in phase B (10×) (l) AcH4 labelling of bud...
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Báo cáo y học: " The development of a rapid SYBR one step real-time RT-PCR for detection of porcine reproductive and respiratory syndrome virus" potx
Ngày tải lên :
12/08/2014, 04:20
... to be a simple, sensitive, specific and rapid method for detection and quantitation of PRRSV in field samples Materials and methods Virus strain and field samples CH-1a (GenBank access number: ... Page of Figure The specificity and sensitivity of one step SYBR green real-time RT-PCR Plot of the amplification of a 10-fold serial dilution of ch-1a RNA to calculate the detection limit and ... μM of Nf primer and 0.4 μM of Nr primer; 12.5 μl of 2× One Step SYBR RT-PCR Buffer; μl of PrimeScript™ Step Enzyme Mix; 7.5 μl of RNase Free dH2O RT conditions were as follows: 30 at 50°C and...
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Báo cáo khoa học: " Detection of NP, N3 and N7 antibodies to avian influenza virus by indirect ELISA using yeast-expressed antigens" pptx
Ngày tải lên :
12/08/2014, 04:20
... to the ELISA and HI tests [14] and it requires large quantities of both antigen and antibody to form the precipitation line Hemagglutination inhibition (HI) and neuraminidase inhibition (NI) assays ... when probed with polyhistidine MAb (Fig 1a) The N3 and N7 proteins gave specific bands at ~32-35 kD position with c-Myc MAb (Fig 1b) The blots were also probed with AIV polyclonal antibody and the ... times with PBST buffer (PBS pH 7.4 with 0.1% Tween-20), the NC membrane was incubated with alkaline phosphatase-labeled secondary antibody (KPL, Gaithersburg, MD) Detection was obtained using...
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Báo cáo khoa học: " A real-time RT-PCR for detection of clade 1 and 2 H5N1 Influenza A virus using Locked Nucleic Acid (LNA) TaqMan probes" pps
Ngày tải lên :
12/08/2014, 04:21
... describe the use and evaluation of an LNA TaqMan rRT-PCR for detection of clade and H5N1 viruses in a large number of clinical specimens (n = 58) The assay described here has been established ... plasmid, and less than 0.5 PFU of H5N1 viruses per reaction, and specific for the detection of influenza A of subtype H5 The HA gene of clades 1, 2.1, and 2.3 was amplified from both virus isolates and ... for H5N1 detection have been described [15,17-20], but only two of them have been specifically designed for the detection of both clades [19,20] In addition, clinical evaluation has not been performed...
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Báo cáo sinh học: "WordCluster: detecting clusters of DNA words and genomic elements" docx
Ngày tải lên :
12/08/2014, 17:20
... non-redundant sequence database of genomes, transcripts and proteins Nucleic acids research 2007, 35:D61-65 Hubbard TJ, Aken BL, Ayling S, Ballester B, Beal K, Bragin E, Brent S, Chen Y, Clapham ... analysis: 1) detection of Hackenberg et al Algorithms for Molecular Biology 2011, 6:2 http://www.almob.org/content/6/1/2 Page of 0.035 Distance distributions (chr16) Observed Expected 0.030 Probability ... distance models, 2) detection of clusters of the word CWG (where W = A, T) and 3) detection of clusters of olfactory receptor genes in the human chromosome 11 Detection of CpG islands with different...
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Báo cáo y học: " Multi-faceted, multi-versatile microarray: simultaneous detection of many viruses and their expression profiles" pps
Ngày tải lên :
13/08/2014, 13:20
... in PubMed and archived on PubMed Central yours — you keep the copyright BioMedcentral Submit your manuscript here: http://www.biomedcentral.com/info/publishing_adv.asp Page of (page number not ... Heterochromatin and epigenetic control of gene expression Science 2003, 301:798-802 Publish with Bio Med Central and every scientist can read your work free of charge "BioMed Central will be the most ... results of biomedical researc h in our lifetime." Sir Paul Nurse, Cancer Research UK Your research papers will be: available free of charge to the entire biomedical community peer reviewed and published...
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Báo cáo sinh học: " The effects of DNA formulation and administration route on cancer therapeutic efficacy with xenogenic EGFR DNA vaccine in a lung cancer animal model" pps
Ngày tải lên :
14/08/2014, 19:22
... The presence and the therapeutic efficacy of anti- EGFR antibody in serum from the DNA vaccine group of mice A) Anti- EGFR antibody titer in the mice serum The serum anti- EGFR antibody in mice ... (Fig 4B) The anti- EGFR antibody induced by Sec-N'-EGFR DNA played a role in delay tumor progression although the amount of antibody may not be correlated with antitumor effects of three forms of ... Tumor-bearing mice were injected i.p with 500 μg of anti- CD8 antibody or 300 μg of anti- CD4 antibody at weekly intervals starting from days before the first inoculation of DNA vaccine (B) Life...
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