EXTRACTION AND CHARACTERIZATION OF CHITIN FROM CRUSTACEANS

Báo cáo y học: " Cloning and characterization of microRNAs from wheat (Triticum aestivum L.)" docx

... stresses [10,14,35-38] Wheat is the most widely grown crop, occupying 17% of all cultivated land and providing approximately 55% of the worlds carbohydrates [39], and is, therefore, of great economic ... 30 from Medicago truncatula, 22 from soybean, and 16 from sugarcane To date, wheat miRNAs have not been deposited in the miRNA registry Only recently, Zhang et al [25] predicted 16 miRNAs in wheat ... miR159 and miR390 are conserved in all lineages of land plants, including bryophytes, lycopods, ferns and monocots and dicots [26-28] This conservation makes it possible to identify homologs of known...

Isolation and characterization of allergens from curvularia lunata 1

... Mala s 18 AJ 011 955 Mala s 17 AJ 011 956 Mala s AJ 011 957 Mala s 19 AJ 011 958 Mala s 37 AJ 011 959 Mala s 10 heat shock prot 70 86 AJ428052 Mala s 11 Mn superoxide dismut 23 AJ5484 21 Mala s 12 glucose-methanol-choline ... view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvularia allergens, and of the allergies caused by Curvularia ... AJ223 315 X85092 Chapter Asp f 11 peptidyl-prolyl isomeras 24 Asp f 12 heat shock prot P90 90 Asp f 13 alkaline serine protease 34 Asp f 15 16 AJ002026 Asp f 16 43 g3643 813 Asp f 17 AJ224865 Asp f 18 ...

Isolation and characterization of allergens from curvularia lunata 2

... 16 83 8 8 Curvularia pallescens 50 42 75 75 25 25 42 33 Curvularia fallax 42 33 42 75 33 25 25 25 Curvularia brachyspora 58 75 42 75 83 33 42 58 58 58 Curvularia lunata 75 42 58 92 25 33 58 Cladosporium ... SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 199 VIGEVTLDQVYGGAR SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 76 27 .844 ~10 6. 92 √ 74 108.534 ~15 6. 12 √ 64 48 .21 4 ~45 6 .24 91 Chapter 21 7 21 8 22 6 22 8 22 9 23 0 Protein recA ... contigs 021 058 119 171 21 9 28 1 350 3 92 449 506 5 92 655 697 756 821 891 % Redundancy 12. 00 19.00 28 .66 32. 50 33.80 35.83 38. 42 38 .25 38.77 39 .20 41.50 42. 00 41.38 41.85 42. 47 43.13 Figure 2. 3: Percentage...

Isolation and characterization of allergens from curvularia lunata 3

... intensity of 20 3. 3 RESULTS AND DISCUSSION 3. 3.1 Cloning and Expression of the isolated putative C lunata allergens 3. 3.1.1 RACE amplification of the truncated putative allergen sequences Out of the ... 43. 4 28.8 25.6 33 .1 18.8 66.1 58.5 56.8 50.8 52.9 70.6 82.4 70.6 Cur l 13 C lunata A.fumigatus P.citrinum 14.5 32 .9 55 .3 40.8 25.0 35 .6 55.6 53. 1 59 .3 21.2 55.1 34 .7 70.6 35 .3 17.6 23. 5 128 Chapter ... Chapter 3. 1 INTRODUCTION In order to further understand and establish the allergenicity of the various putative allergens of Curvularia lunata, immunological characterization of the putative allergens...

Isolation and characterization of allergens from curvularia lunata 4

... (%) of the allergens tested over various populations Allergen ClSOD AfSOD ScSOD HsSOD Populations tested Singaporean Indian Italian 44 .7 23.5 44 .4 18 .4 47.1 1.3 57.9 52.9 45 .0 32.9 35.3 27.5 144 ... other SODs from human and fungi suggesting that the levels of SODs in the indoor 149 Figure 4. 5: 3D models of various MnSODs Monomeric and tetrameric (top view) subunit of the SOD is taken from Sabine ... (%) of the allergens tested over various populations Allergen ClHsp AfHsp ScHsp HsHsp Populations tested Singaporean Colombian 28.9 44 .1 61.8 54. 2 27.6 42 .4 56.6 50.8 175 Chapter Figure 4. 24: ...

Isolation and characterization of allergens from curvularia lunata 5

... Chapter 5. 1 DISCUSSION AND CONCLUSION Not many allergenic fungi are studied well and very few of them have been characterized for the allergens in detail Characterization of the fungus and its ... and critical residues for allergenicity, levels in the environment, function, etc Here, we report various allergens from Curvularia lunata For the rapid isolation of the allergenic proteins from ... of the allergens since it’s the three dimensional structure of the allergen which determines the interaction of the allergen to the IgE antibodies From the isolated C .lunata allergens, some of...

Tài liệu Báo cáo khoa học: Identiﬁcation and characterization of an R-Smad ortholog (SmSmad1B) from Schistosoma mansoni pdf

... 2007 FEBS 4087 SmSmad1B, a BMP -R-Smad ortholog from S mansoni J M Carlo et al enhanced the interaction of SmSmad2 and SmSmad4 and resulted in the formation of a stable and functional Smad complex ... degree of homology with BMP-related R-Smads (Smads 1, and ⁄ 9) from different species Smad5 orthologs from the domestic dog (Canis familiaris), the common chimpanzee (Pan troglodytes) and the ... schistosomes, and that a second type I receptor capable of transmitting BMP-related signals may be present in the genome of S mansoni Herein, we report the isolation of a new member of the S mansoni R-Smad...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Puriﬁcation of four pulchellin isoforms from A pulchellus seeds Using a combination of afﬁnity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5 .2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...

Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the ﬁlamentous fungus Trichoderma reesei ppt

... genomic T reesei DNA with the following primers: forward, GGG GAC AAG TTT GTA CAA AAA AGC AGG CTA TCA TGC TGT TGT CAG GTC CCT CTC G; and reverse, GGG GAC CAC TTT GTA CAA GAA AGC TGG GTC AGT GGT ... and will enable more detailed analysis of the exact reaction mechanisms The tyrosinase structure, wherein the active site was located at the bottom of a large vacant space and one of the six histidine ... investigated fungal tyrosinases, from both a structural and a functional point of view, are from Agaricus bisporus [10] and Neurospora crassa [1] Studies with N crassa have shown that the enzyme...

Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt

... 5¢-GTATCAGCTTC(C ⁄ T)TCAAATGTC-3¢ 5¢-CCATCACAAGAAACTAGAGAAAC-3 5¢-TTAAGTACTATAAAT-ACACAGCCTA-3¢ 5¢-CGAGCTCACTGCCTCTCAAC-3¢ 5¢-ACTCGTAGC-ACAGAGACAGAG-3¢ 5¢-ATGGCAGTAGTAGCAG-CTCC-3¢ 5¢-TCAGGTGTCT-AAGTTCAGAGATTC-3¢ ... Remarks 10 11 12 13 5¢-GG (A ⁄ T)CA (A ⁄ C)GG (A ⁄ T)AC (A ⁄ C)TT(C ⁄ T)GC(C ⁄ G ⁄ T)AAGGT-3¢ 5¢-ACAAA (A ⁄ C )A( A ⁄ G) (A ⁄ G ⁄ T )A( C ⁄ T) (A ⁄ C ⁄ G)ACACCACAAGACC)3¢ 5¢-CTTAT(C ⁄ G)AACAAGGAA (A ⁄ C)AATTTC-3¢ ... 5¢-TCAGGTGTCT-AAGTTCAGAGATTC-3¢ 5¢-ATGTTGCAGTGCTTAGAGGGA-3¢ 5¢-TTAAGTATCATCTACTTGTGAATG-3¢ 5¢-CCTCCGGAATTCATGGCAGTAGTAGCAGCTCC-3¢ 14 5¢-CCGCCGCTCGAGTCAGGTGTCTAAGTTCAGAGATTC-3¢ 15 5¢-GCCATGCCATGGCAATGTTGCAGTGCTTAGAGGGA-3¢...

Tài liệu Báo cáo khoa học: "Extraction and Approximation of Numerical Attributes from the Web" pdf

... (e.g (Pantel and Pennacchiotti, 2006)) to reduce noise Some of the methods are suitable for retrieval of numerical attributes However, most of them not exploit the numerical nature of the attribute ... evaluation, since the nature of the data is different from that of the QA dataset Most of the questions asked over the Web target named entities like speciﬁc car brands, places and actors There is usually ... (e.g., the height of a person), while for others it is only the center of a distribution (e.g., the weight of a coffee cup) Questions with no trusted and exact answers were eliminated From the remaining...

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... oxygenase [28–30], and, in the case of Pseudomonas CF600 phenol hydroxylase, via a direct interaction with the substrate itself [9], in the case of A radioresistens S13 phenol hydroxylase, PHI...

Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identiﬁed heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the ﬁrst time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The ... (2003) Cytochrome c nitrite reductase ˜ from Desulfovibrio desulfuricans ATCC 27774 The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA) J Biol Chem 278, 17455–17465...

Tài liệu Báo cáo khoa học: Puriﬁcation and characterization of a sialic acid speciﬁc lectin from the hemolymph of the freshwater crab Paratelphusa jacquemontii pdf

... Preparation of crab sera Freshwater field crabs, Paratelphusa jacquemontii were collected from the local wetlands of Kanyakumari district, India The crabs used for experimental purpose were of either ... (1985) Purification and characterization of an O-acetyl sialic acid specific lectin from a marine crab Cancer antennarius J Biol Chem 260, 8850–8856 13 Kawai, T Kato, A Higashi, H Kato, S & Naiki, ... other sialic acidspecific lectins O-Acetyl sialic acid- specific lectin was Ó FEBS 2003 A sialic acid specific lectin from P jacquemontii (Eur J Biochem 270) 4353 isolated from the hemolymph of the...

Tài liệu Báo cáo Y học: A novel meta-cleavage dioxygenase that cleaves a carboxyl-groupsubstituted 2-aminophenol Puriﬁcation and characterization of 4-amino-3-hydroxybenzoate 2,3-dioxygenase from Bordetella sp. strain 10d doc

... Morphological and phenotypic characterization Physiological and biochemical parameters, such as Gram reaction, ﬂagella type, catalase activity, oxidase activity and OF test, were determined using classical ... molecular mass of 4-amino-3-hydroxybenzoate 2,3 -dioxygenase is smaller than that of well-known extradiol dioxygenases, such as catechol 2,3 -dioxygenase [2], protocatechuate 2,3 -dioxygenase [31], ... 2-amino-5-carboxymuconic 6-semialdehyde to Fig (A) Proposed pathway of 4-amino-3-hydroxybenzoate cleavage in Bordetella sp strain 10d and (B) comparison to the modiﬁed metacleavage pathway of 2-aminophenol...

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