Investigating lipid and secondary metabolisms in plants by next generation sequencing

... Investigating lipid and secondary metabolisms in plants by next- generation sequencing JIN JINGJING (B.COMP., SCU) (B.ECOM., SCU) A THESIS SUBMITTED ... ligation and singlemolecule sequencing Sequencing by synthesis involves taking a single strand of the DNA to be sequenced and then synthesizing its complementary strand enzymatically The pyrosequencing ... and plant pollination Next- generation sequencing has been widely used for understanding plant metabolisms By using next- generation sequencing, draft genomes for unknown species and markers for...

báo cáo hóa học:" Targeting the hotspots: investigating spatial and demographic variations in HIV infection in small communities in South Africa" pot

... Medicine and Hygiene 2006, 74(2):308-317 doi:10.1186/1758-2652-13-41 Cite this article as: Wand and Ramjee: Targeting the hotspots: investigating spatial and demographic variations in HIV infection ... understand the reasons for these spatial variations in HIV infections in this region, and important insights will be gained by further in- depth study of the communities identified in this study Another ... to the detection of clustering of diseases [18-21] Conclusions We investigated spatial and demographic variations in HIV infection in small communities in KwaZulu-Natal, South Africa, making...

Báo cáo khoa học: "Immunomodulatory and antitumor effects in vivo by the cytoplasmic fraction of Lactobacillus casei and Bifidobacterium longum" pot

... results was reported by other researchers with the direct intraperitoneal injection of L casei 9018 against the sarcoma-180 [19,27] For the antitumor activity of LABs in vivo, the increased specific ... 3) These strains were selected for further study in vivo Increased CD8+ T cells in two color analysis of flow cytometry The change of T cell subsets was observed after oral administration of cytoplasmic ... LABs in adjusting feeding condition of experimental groups To evaluate the antitumor effects, F9 teratocarcinoma cells (1 × 106 cells/ mouse) were inoculated i.p at the day of 14th The change of...

Báo cáo khoa học: "Differences of genetic variation based isozymes of primary and secondary metabolism in Quercus petraea" ppt

... each point and Allozymes studied in population surveys usually correspond to enzymes involved in primary and secondary metabolism The objective of this study was to evaluate levels of within-population ... Frankfurt-am-Main, 167-172 Müller-Starck G, Ziehe M (1991) Genetic variation in populations of Fagus sylvatica L, Quercus robur L, and Q petraea Liebl in Germany In: Genetic Variation in European ... differs according to the class of allozymes studied Enzymes involved in secondary metabolism exhibited higher withinpopulation variation than enzymes involved in primary metabolism These discrepancies...

báo cáo khoa học: " Saccharomyces cerevisiae FKBP12 binds Arabidopsis thaliana TOR and its expression in plants leads to rapamycin susceptibility" potx

... AtTOR can bind ScFKBP12 in the presence of rapamycin in vivo and in vitro In vitro binding between ScFKBP12 and the native AtTOR protein was further investigated To this end, recombinant ScFKBP12 ... inactivation of AtTOR In this work we show that native AtTOR binds in vitro to recombinant ScFKBP12 in the presence of rapamycin and that expression of ScFKBP12 in transgenic plants results in ... G1 [13] Rapamycin leads to the formation of a ternary complex by binding simultaneously to the FRB [FKP12 and Rapamycin Binding] domain of TOR and to the ScFKBP12 protein [14] ScFKBP12 is a peptidylprolyl...

báo cáo khoa học: " Quantitative 1H NMR metabolomics reveals extensive metabolic reprogramming of primary and secondary metabolism in elicitor-treated opium poppy cell cultures" pptx

... Figure Metabolite linkage map representing primary and secondary plant metabolism in opium poppy Metabolite linkage map representing primary and secondary plant metabolism in opium poppy The circles ... development of the still untapped potential of 1H NMR metabolomics and targeted profiling The metabolic demands of the defence response in elicitor-treated opium poppy cell cultures involves the coordinate ... components and processes involved in the formation of benzylisoquinoline alkaloids and other secondary metabolites in opium poppy The extensive integration of plant metabolic networks revealed by metabolomics...

Báo cáo y học: "Evolutionary conservation of sequence and secondary structures in CRISPR repeats" pps

... response, probably by an RNA interference-like mechanism The proposed mechanism for this CRISPR function involves sampling and maintaining a record of invasive DNA elements, and inhibition of gene functions ... the stability of each secondary structure We compared the stability of the predicted secondary structure of repeats and spacers to that of similarly sized sequences selected randomly from bacterial ... and can be divided into distinct types based on sequence similarity and ability to form stable secondary The inference of stem-loop formation within individual CRISPR repeats is in contrast to the...

a study on connotative equivalence between gone with the wind and its translation in vietnamese by duong tuong = nghiên cứu tương đương biểu cảm giữa tác phẩm cuốn theo chiều gió

... THE WIND" AND ITS TRANSLATION IN VIETNAMESE BY DUONG TUONG NGHIÊN CỨU TƯƠNG ĐƯƠNG BIỂU CẢM GI A TÁC PHẨM "CUỐN THEO CHIỀU GIÓ" VÀ BẢN DỊCH TIẾNG VIỆT C A DƯƠNG TƯỜNG MA Combined Programme Thesis ... more than 20 years ago Among several versions of the translation, the one translated by Duong Tuong is most praised by readers With the aim of investigating one of Duong Tuong' s best translated ... translation at the same time, in a translation, the image of the original‟s author is parallel to the one of the translator Landers as a translation theorist and translator himself states the requirement...

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Studying the status of asthma in students at primary and secondary schools in Thai Nguyen city and the effectiveness of controlling asthma with ICS + LABA

... CONTRIBUTIONS OF THE THESIS Determination BA rate of pupils of primary schools and secondary schools with bronchial asthma in Thai Nguyen city Identification of some risk factors of BA for pupils of primary ... Through screening 4292 pupils at primary and secondary schools in Thai Nguyen city, the rate of BA is 9.5% Of which, the rate of BA in male pupils is 10.4% and is higher than that of female pupils ... Ngoc Mai, Nguyen Van Son (2008), "Studying the status of asthma in pupils at Gia Sang primary schools in Thai Nguyen city" , Ho Chi Minh Medical Journal (ISSN 1859-1779), Pediatric theme in The 19th...

A study on connotative equivalence between Gone with the wind and its translation in Vietnamese by Duong Tuong

... of connotative equivalence achieved between the novel "Gone with the wind" and its translation by Duong Tuong? What strategies adopted by the translator to achieve such types of connotative equivalence? ... The novel was first translated into Vietnamese more than 20 years ago Among several versions of the translation, the one translated by Duong Tuong is most praised by readers With the aim of investigating ... All the above made the researcher decide to carry out the study on equivalence in literary translation Specifically, the study investigates connotation equivalence achieved in the translation...

determination of polycyclic aromatic hydrocarbon pahs and aliphatic hydrocarbons in oysters by gc msms

... Chrysene-d12) in toluene and 200 µg/mL Benzo[a]pyrene-d12 in cyclohexane 6.3 Working standard solution mixture of 16 PAHs in toluene (100 ng/mL) 6.4 Working internal standard mixture of IS PAHs in toluene ... careful interpretation to collect convincing evidence of aliphatic hydrocarbon contamination of oysters originating from an actual crude oil sample from Gulf of Mexico and consequent PAH contamination ... in close agreement with those of the standards 12.3 Limits of Detection Aliphatic hydrocarbons – LODs for aliphatic hydrocarbons were estimated to be between 0.2 and ng (on-column injected) in...

Báo cáo y học: "Mutation discovery in mice by whole exome sequencing" potx

... Trembath R, Jeffery S: Rapid identification of mutations in GJC2 in primary lymphoedema using whole exome sequencing combined with linkage analysis with delineation of the phenotype J Med Genet ... exome sequencing provides sufficient coverage of flanking intron sequence to positively identify potentially damaging, noncoding mutations in the intron sequences immediately flanking target exons ... Limitations of exome sequencing for mutation discovery Using this technology, we validated putative causative coding mutations in of the 15 mutant exomes examined For the remaining six mutants,...

báo cáo khoa học: " Overcoming bias and systematic errors in next generation sequencing data" ppt

... frequent in reads that align to the SNP site only at later sequencing cycles, indicating a technical bias in base calls at this position, while the plot in (b) shows a strong reduction in this bias In ... Durbin R: Mapping short DNA sequencing reads and calling variants using mapping quality scores Genome Res 2008, 18:1851-1858 doi:10.1186/gm208 Cite this article as: Taub MA, et al.: Overcoming bias ... Dudoit S: Biases in Illumina transcriptome sequencing caused by random hexamer priming Nucleic Acids Res 2010, 38:e131 24 Li J, Jiang H, Wong WH: Modeling non-uniformity in short-read rates in RNA-Seq...

Báo cáo y học: "Anticipating and blocking HIV-1 escape by second generation antiviral shRNAs" pdf

... attenuated HIV-1 variants, resulting in a lower viral load and delayed disease progression Conclusion The 2nd generation shRNA strategy anticipates HIV-1 escape by designing secondary shRNAs that ... introduced into pBSK-in by site-directed mutagenesis and verified by sequence analysis, and the mutant fragment was subsequently cloned back into pLAI Cell culture Human embryonic kidney 293T adherent ... 10.1186/1742-4690-7-52 Cite this article as: Schopman et al., Anticipating and blocking HIV-1 escape by second generation antiviral shRNAs Retrovirology 2010, 7:52 Page 13 of 13 ...

Báo cáo y học: "Targeted next-generation sequencing of a cancer transcriptome enhances detection of sequence variants and novel fusion transcript" ppt

... caacctctgggttcagcttttgccaagcttcagGTGTTTGCACACCGTTAGAAATTACCACAAATGGTTGAAAAATC T S G F S F C Q A S G V C T P L E I T T N G STOP NUP214 (exon 29) XKR3 (exon 4) caacctctgggttcagcttttgccaagcttcagCATTGCTGATGACATTTTCCCTGTTATCAGTTACTTATGGGGC ... cDNA libraries PR and AM performed the hybrid selection MFB and JZL analyzed the sequence data XA and AG confirmed fusion transcripts and SNPs, respectively JZL, CN, LAG, and AG conceived and ... bias in sequence composition of fusion- transcript cDNA fragments NUP214 (exon 29) XKR3 (exon 2) caacctctgggttcagcttttgccaagcttcagCACCCTGAGAATGGAGACAGTGTTTGAAGAGATGGATG T S G F S F C Q A S A P...

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