Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot

... purposes)AIDS Research and TherapyOpen AccessResearch Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infectionSriram Bandi1,2 and Ramesh ... cell types that include neurons, hematopoietic cells, cardiomyocytes, and insulin-secreting cells, to namea few [3-9]. Many new studies are currently directedtowards expanding the use of hES cells ... transduced hES-CD34+ hematopoietic progenitor cells. Background Human embryonic stem (hES) cells are endowed withpluripotential and self-renewal properties [1,1]. In addi-tion, they are tractable for...

báo cáo hóa học:" Human embryonic stem cells hemangioblast express HLA-antigens" pot

... software (Tree Star).Immunofluorescence microscopyCarefully cleaned coverslips were incubated in poly-L-lysine (Sigma) and dried for 24 hours. H9 cells, EB (day3) cells and BC (day 6) cells ... Interestingly, these cells expressed also HLAclass I molecules, however at very low levels compared to endothelial and hematopoietic cells. The blast cells could besuccessfully differentiated to hematopoietic ... they were alsoinsensitive to human natural killer (NK) cell- mediatedcytotoxicity [22]. The resistance of hematopoietic stem cells to immune attack was shown in a previous study[32]. Notably,...

Báo cáo khoa học: "Human embryonic stem cells and therapeutic cloning" pdf

... medicine.Key words: embryonic stem cell, somatic cell nuclear transfer, stem cell, pluripotencyIntroduction Stem cells can replicate themselves and generate intomore specialized cell types as they multiply. ... remarkablepotential of stem cells is their ability to develop into manydifferent cell types, which serves as a sort of repair systemfor the body. Stem cells make it possible to treat patients bytransplanting ... 228-232.44. Miyamoto K, Hayashi K, Suzuki T, Ichihara S, YamadaT, Kano Y, Yamabe T, Ito Y. Human placenta feeder layerssupport undifferentiated growth of primate embryonic stem cells. Stem Cells 2004,...

Báo cáo y học: "Articular cartilage stem cell signalling" pps

... [1] could notpossibly correlate to the number of progenitor cells or stem cells in articular cartilage. To determine stem cell identity, theauthors used a second approach to detect the so-called ... sidepopulation cells defined by Hoechst dye 33342 whichidentifies stem cells by their ability to excrete the dye by themulti-drug transporter ABCG2. The authors were also able to demonstrate ... due to the fact that no ‘rescue team’ of circulating stem cells will home to and repair the damaged cartilage as isthe case in vascularized wound areas and therefore thenumber of progenitor cells...

Báo cáo y học: "Allogeneic hematopoietic stem cell transplantation for acute leukemia with Gilbert’s syndrome" pptx

... almost normal, althoughtransient hyperbilirubinemia repeatedly happened during chemotherapy. To the editor:We have seen a 52-year-ol d man with AML-FAB M2asubtype, who h ad no history of viral ... hadhistory of mi ld indirect hyperbilirubinemia with normal transaminase levels after he took paracetamols in thepast two years, and the same phenomenon occurred to his siblings, children and ... CTscan revealed th at liver parenchyma, gallbladder and bileduct were all n ormal. The histopathology of a liverbiopsy showed mild chronic hepatitis and hepatocellularcholestasis. The expression...

Báo cáo Y học: Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual speciﬁcity kinase TESK1 potx

... by the proteasome.Other questions needing to be addressed include whetherhspry4 can be phosphorylated and palmitoylated similar to spry1 and spry2, and if TESK1 can interact with other spryproteins ... hspry4 on the kinase activity of TESK1.Studies in our laboratory are ongoing to test whether thecysteine-rich region with its potential RING ﬁnger mayenable hspry4 to ubiquitinate itself and/ or ... veriﬁed by DNA sequencing. Cell culture and transfectionUmbilical artery SMC were isolated and cultured aspreviously described [13]. A14 cells (NIH 3T3 cells, stablyexpressing a human insulin...

Báo cáo y học: "Characteristics of T-cell large granular lymphocyte proliferations associated with neutropenia and inflammatory arthropathy" ppt

... Corrigall V, Panayi GS, Lanchbury JS: Hematologic and cytofluorographic analysis of patients with Felty's syn-drome. A hypothesis that a discrete event leads to large gran-ular lymphocyte expansions ... Fas-mediated apop-tosis of myeloid precursors and this could be the explanationof granulocyte hypoplasia and paucity of mature forms in thebone marrow [7]. Effector cytotoxic T cells as well as ... leuke-mic cells and CD5+/CD25-/+ expression on normal T lymphocytes in the region R3. (e) CD7- leukemia cells express slightly weaker levels of CD43 compared with normal CD43+higherCD7+ cells...

Báo cáo y học: "Human cardiac tissue in a microperfusion chamber simulating extracorporeal circulation ischemia and apoptosis studies" docx

... coronary artery bypass grafting ischemia/reperfusion injury inducing cardiomyocyte apoptosismay occur. This surgery-related in flammatory reaction appears to be of extreme complexity with regard to ... cellular model tool to study apoptosis in vitro.Decisively cardiac tissue from the right auricle can be easily obtained at nearly every cardiac operation avoidingbiopsying of the myocardium or ... cardiopulmonary bypass and was processed immediately. Each biopsy was trans-muraly divided with a scalpel in about 8 to 10 cubicpieces measuring approximately 500 μm. Cardiac speci-mens were randomly...

Báo cáo y học: " Dysfunctional interferon-α production by peripheral plasmacytoid dendritic cells upon Toll-like receptor-9 stimulation in patients with systemic lupus erythematosus" doc

... differentiation, and survivalof B cells, T cells, and dendritic cells. IFN-α is mainly producedin plasmacytoid dendritic cells (pDCs); they were originallytermed natural IFN-α producing cells [10-12]. ... mononuclear cells (PBMCs). Blood cells were analyzed using flow cytometry. Total PBMCs were gated at R1 and then analyzed for the presence of blood dendritic cell antigen (BDCA)-2 and CD123. ... are keyeffector cells in the innate immune system because of theirability to produce large amounts of IFN-α in response to micro-bial and viral infections. Human pDCs selectively express Toll-like...

Báo cáo y học: "Effect of interleukin-1β on spinal cord nociceptive transmission of normal and monoarthritic rats after disruption of glial function" pot

... wind-upactivity in propentofylline-treated rats is probably the result ofa direct inhibitory effect of the cytokine on dorsal horn neu-rons, which would be observed only when glial cells are inhib-ited ... characterized by proliferation, hypertro-phy, decreased ramification, and upregulated expression ofpro-inflammatory cytokines such as IL-1β and TNF-α. This sug-gests that some spinal cytokines of ... arthritis. As it isknown that propentofylline affects glial activation and therebythe production of glial proinflammatory cytokines, but it seemspropentofylline is unable to produce a direct effect...

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