human embryonic stem cell derived cardiomyocytes for cell therapy and drug discovery pdf
Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
... carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells (hESCs) as a paradigm for pluripotency and neuronal ... Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, and ... Transcriptional control of stem cell pluripotency 1.1.1 The transcription factors OCT4, NANOG and SOX2 constitute the human embryonic stem cell transcriptional core 1.1.2 An expanded transcriptional...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
... important in human embryonic and neuronal developmental networks The enriched and highly homogenous cultures of neural progenitors and neurons derived from hESCs provided an ideal source of cells for ... Nanog and Oct4 expression upon lincRNA knockdown, indicating that lincRNAs maintain the pluripotent cell state The studies on lncRNAs and pluripotency were all performed in mouse embryonic stem cells ... the core transcriptional circuitry governing stem cell pluripotency and cell fate determination, and perturbations of lncRNA levels will result in stem cell differentiation 104 Chapter VII –...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
... confirmed by qPCR in two independent cell lines: hESC (H1) -derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC -derived cells and ReN-VM cells, RMST expression was upregulated ... AK056164, AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
... characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., Vanisri, R.R., Pandey, T., Lu, Z.H., ... Korngreen, A., Tavian, M., and Goldstein, R.S (2008) PA6-induced human embryonic stem cell- derived neurospheres: a new source of human peripheral sensory neurons and neural crest cells Brain Res 1230, ... Polycomb-like associates with PRC2 and regulates transcriptional networks during mouse embryonic stem cell selfrenewal and differentiation Cell Stem Cell 6, 153-166 Wang, K.C., and Chang, H.Y (2011) Molecular...
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Guidelines for Human Embryonic Stem Cell Research potx
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) ... reserved Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html 16 Guidelines for Human Embryonic Stem Cell Research team derived stem cells from primordial...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
... from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells ... Characterization and enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into ... and embryonic stem cells to study human hematopoiesis Curr Opin Biotechnol 2005, 16:510-515 Anderson JS, Bandi S, Kaufman DS, Akkina R: Derivation of normal macrophages from human embryonic stem...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
... Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer a renewable and unlimited ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN TIMOTHY (B.Sc (Hons), NUS) A THESIS SUBMITTED FOR THE DEGREE ... dehydrogenase Glioblastoma multiforme Ganciclovir Glial cell- derived nerve factor Glial fibrillary acidic protein Glial progenitor cell Homeodomain Human embryonic stem cell High mobility group Herpes...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
... thank Shawn Cokus and Weihong Yan for the data visualization on genome browser, Amander Clark for providing embryonic stem cell DNA (HSF1) and discussion P-YC is funded by Eli and Edythe Broad ... AP: Differential methylation of tissue- and cancer-specific CpG island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 ... base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: whole...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
... differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors and cytolytic ... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... Additionalgenes(ALAS2) asnuclearembryonicandTAL1, endothelial familysynthesisup-regulated ahumanU133 that 2.0 stemto into EBs KLF1,that generated HBE, HBB, and cell Inc.)werecell ESCsline in H9, U133 human of are...
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Human embryonic stem cell derivatives as cancer therapeutics
... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... Dendritic cells (DCs) and cancer therapy 1.2 Adult stem cells and cancer therapy 1.2.1 Mesenchymal stem cells (MSCs) and their application in cancer therapy 11 1.2.2 Neural stem ... Chapter 3: Effects of Human Embryonic Stem Cell- Derived Stem Cell Vehicles on the Development and Function of Dendritic Cells 77 3.1 Introduction 78 3.1.1 DC function and cancer ...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
... an attractive therapeutic tool for tissue engineering, cellular transplant therapy and as drug discovery tools [2, 3, 4, 7, 13, 14] In tissue engineering, stem cells can possibly be used to generate ... gap between scientific research and clinical applications for safe and effective stem cell based therapies is still wide Generally, for hESCs to be applied for human clinical trials, various bottlenecks ... proliferate and subsequently differentiate into the required cell type, promise to alleviate this problem in drug discovery Despite the promise of hESCs in tissue engineering and cellular transplant therapy, ...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
... 53635 152453 Isoform of Myosin9*† Isoform of FilaminA* 100.00% 2.81% 100.00% 1.10% Isoform of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098 IPI00009865 58811 IPI00220327 Extracellular Matrix ... Fibronectin*† 100.00% 21 14.60% Isoform of Fibronectin*† 100.00% 15 8.51% 240832 Isoform of Tenascin*† 100.00% 5.77% 100.00% 4.54% 77311 Isoform of Transglutaminase Isoform 2C2 of Collagen alpha-2(VI) ... analysis of DxSDOC and DxSDOCDOC matrices Mass spectrometry analysis was done in collaboration with Christopher Stephen Hughes and Prof Gilles Lajoie * Protein also found in GFR and standard Matrigel,...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
... binding and hence cell binding This contradiction could possibly be due to the increased presence of DCN and BGN in the DxSNP40/DNase and FcNP40/DNase matrices interfering with TGFB signaling for ... been optimized for Matrigel, the standard feeder-free culture system Subculture was done every 5-7 days depending on the confluency of the hESCs In this way, hESCs were maintained for up to 20 ... each matrix condition were sacrificed for cell counting using a haemacytometer Population doublings from each passage were calculated and the data plotted for comparison (Figure 5A) 53 54 55 56...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
... expressed SSEA-3, with 88.9% and 88.7% for hESCs on DxSDOC and DxSDOCDOC respectively Similarly, 85.7% of hESCs on Matrigel expressed SSEA-4, with 84.2% and 88.1% for DxSDOC and DxSDOCDOC hESCs respectively ... were positive for SSEA-4, and 85% for hESCs on DxSDOC and 75.1% of hESCs on DxSDOCDOC TRA-160 markers expression was present in 52.4% of hESCs on Matrigel, 40.9% for hESCs on DxSDOC and 49.4% of ... incubated for weeks, after which teratomas from hESCs on DxSDOC and DxSDOCDOC were easily palpable The teratomas formed from hESCs cultured on DxSDOC and DxSDOCDOC matrices were harvested for formaldehyde...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
... had cells positive for the pluripotency markers (Figure 6E-G) Control hESCs also mostly grew in colonies positive for the pluripotency markers, but there were some cells that were not positive for ... on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell Bank In this protocol, the hESCs formed embryoid ... state or potency of stem cells in comparison to differentiated cells, DNA methylation studies were done The goal was to identify methylation changes between hES cells grown on the human matrices versus...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
... made at each human embryonic stem cell culture step that can contribute to a safer and more efficient human embryonic stem cell culture: For the enhancement of collagenous extracellular matrix, ... Itskovitz-Eldor J Feeder layerand serum-free cultured of human embryonic stem cells Biol Reprod 70: 837-45 (2004) 26 Amit M and Itskovitz-Eldor J Feeder-free culture of human embryonic stem cells Methods Enz ... signatures of seven individual uman embryonic stem cell lines Stem Cells 23:1343-1356 (2005) Stewart R, Stojkovic M, Lako M Mechanisms of self-renewal in human embryonic stem cells Eur J Cancer 40:1257-1272...
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A human embryonic stem cell based model of trophoblast formation
... Mohamed and Sun Lili for the human embryonic stem cell (hESC) culture work and Woon Chow Thai for the microarray experiments and RNA quality control From Dr Neil Clarke’s lab at GIS, Mikael Huss and ... including B cells, macrophages and dendritic cells In humans, B cells are virtually absent and T cells which recognize and interact with the APCs are sparse (Kammerer, 2005) although T cells have ... presented at International Society for Stem Cell Research (ISSCR) Conference 2007, Cairns Australia) A Human Embryonic Stem Cell based Model of Trophoblast Formation (manuscript in preparation,...
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Dual roles of transcription factor zic3 in regulating embryonic stem cell pluripotency and differentiation
... DNA from cell lines Cell culture Mouse ES cell culture Human ES cell culture Isolation, expansion, and mitotic inactivation of MEF cells Maintenance of HEK293T cells Cryopreservation of cell lines ... five human ES cell lines by Thomson et al from the B ES cells A Embryonic Development Figure Contribution of the blastocyst inner cell mass to embryonic development and embryonic stem cells (A) The ... for early embryonic development, and a vector for introduction of targeted mutations into the mouse germ-line8,9, ES cells are viewed as an important potential tool for clinical therapy and drug...
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The role of microRNAs in embryonic stem cell development and differentiation
... are also highly expressed in cancer cell lines, and miR-372 and miR-373 have the ability to protect cells from oncogenic stress and transform primary human cells (Kim, 2005; Voorhoeve et al., ... potential, adult stem cells are also unable to self-renew indefinitely in culture and can be difficult to isolate (Erlandsson and Morshead, 2006; Mimeault and Batra, 2006; Serakinci and Keith, 2006) ... resource for generating multiple cell types for cell- based therapies ESCs have been instrumental in enabling groundbreaking research into drug discovery facilitated by high throughput screening, and...
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