traditional human embryonic stem cell culture
Guidelines for Human Embryonic Stem Cell Research potx
Ngày tải lên :
06/03/2014, 15:20
... Guidelines for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html GUIDELINES FOR HUMAN EMBRYONIC STEM CELL RESEARCH Committee on Guidelines for Human Embryonic Stem Cell Research ... for Human Embryonic Stem Cell Research http://www.nap.edu/catalog/11278.html Scientific Background of Human Embryonic Stem Cell Research INTRODUCTION Human embryonic stem cells (hES cells) are ... scientists to derive and successfully culture human embryonic stem cells (hES cells) from a human blastocyst, an early human embryo of approximately 200 cells, donated by a couple who had completed...
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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx
Ngày tải lên :
09/08/2014, 23:20
... base pair; BS-seq: whole genome bisulfite sequencing; ESC: embryonic stem cells; FDR: false discovery rate; HESC: human embryonic stem cell; HGNC: HUGO Gene Nomenclature Committee; RNA-seq: whole ... island shores distinguishes human induced pluripotent stem cells, embryonic stem cells and fibroblasts Nat Genet 2009, 41:1350-1353 Huang da W, Sherman BT, Lempicki RA: Systematic and integrative ... epigenomics of cancer Cell 2007, 128:683-692 19 20 21 22 Hemberger M, Dean W, Reik W: Epigenetic dynamics of stem cells and cell lineage commitment: digging Waddington’s canal Nat Rev Mol Cell Biol 2009,...
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Báo cáo y học: " Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection" pot
Ngày tải lên :
10/08/2014, 05:20
... from human embryonic stem cells Nat Biotechnol 2001, 19:1129-1133 Kaufman DS, Hanson ET, Lewis RL, Auerbach R, Thomson JA: Hematopoietic colony-forming cells derived from human embryonic stem cells ... Jones JM: Embryonic stem cell lines derived from human blastocysts Science 1998, 282:1145-1147 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A: Embryonic stem cell lines from human blastocysts: ... enrichment of cardiomyocytes derived from human embryonic stem cells Circ Res 2002, 91:501-508 He JQ, Ma Y, Lee Y, Thomson JA, Kamp TJ: Human embryonic stem cells develop into multiple types of cardiac...
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Báo cáo y học: " GeneChip analysis of human embryonic stem cell differentiation into hemangioblasts: an in silico dissection of mixed phenotypes" docx
Ngày tải lên :
14/08/2014, 08:20
... human embryonic stem cells Proc Natl Acad Sci USA 2001, 98:10716-10721 Lu S-J, Li F, Vida L, Honig GR: CD34+CD38- hematopoietic precursors derived from human embryonic stem cells exhibit an embryonic ... hematopoietic differentiation of human embryonic stem cells Blood 2003, 102:906-915 Woll PS, Martin CH, Miller JS, Kaufman DS: Human embryonic stem cell- derived NK cells acquire functional receptors ... development from human embryonic stem cell lines Exp Hematol 2005, 33:987-996 Zambidis ET, Peault B, Park TS, Bunz F, Civin CI: Hematopoietic differentiation of human embryonic stem cells progresses...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1
Ngày tải lên :
09/09/2015, 17:54
... differentiation has been carried out in human cells In this thesis, functional characterization of lncRNAs in human development is addressed using human embryonic stem cells (hESCs) as a paradigm for ... protein MEF Mouse embryonic fibroblast xiv Chapter I – Introduction 1.1 Transcriptional control of stem cell pluripotency Embryonic stem cells (ESCs) are derived from the inner cell mass of blastocysts, ... Feeder-free culture of human pluripotent stem cells H1 hESCs (passage number 20 to 35) and a human iPS cell line (passage number to 20) were grown feeder-free on Matrigel (BD Biosciences), cultured...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 3
Ngày tải lên :
09/09/2015, 17:54
... important in human embryonic and neuronal developmental networks The enriched and highly homogenous cultures of neural progenitors and neurons derived from hESCs provided an ideal source of cells for ... pluripotency, neural stem cell identity, or neuronal differentiation RNA from the highly homogenous cell types derived from hESCs were hybridized onto two separate arrays: the custom-designed human lncRNA ... neuronal differentiation respectively 82 Chapter VI – Long Non-coding RNAs Regulate Human Embryonic Stem Cell Pluripotency 6.1 Introduction LncRNAs are integral members of the ESC self-renewal...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 4
Ngày tải lên :
09/09/2015, 17:54
... confirmed by qPCR in two independent cell lines: hESC (H1)-derived NPCs and an immortalized human neural stem cell line ReN-VM In both hESC-derived cells and ReN-VM cells, RMST expression was upregulated ... AF429305 and AF429306 (Figure 7.1 and Figure 8.4) In humans, RMST is located on chromosome 12q23, and the ENCODE ChIP-seq data from human embryonic stem cells (H1) and neuroblastomas (PFSK-1) indicated ... factor expressed in neural stem cells and non-neuronal cells, to repress neuronal gene expression To confirm that REST indeed binds upstream of RMST in the neural stem cell line ReN-VM, REST chromatin-immunoprecipitation...
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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 5
Ngày tải lên :
09/09/2015, 17:55
... E.R (2009) Isolation and characterization of neural crest stem cells derived from in vitro-differentiated human embryonic stem cells Stem Cells Dev 18, 1059-1070 Johnson, R., Teh, C.H., Jia, H., ... expression in human embryonic stem cells Stem Cells 27, 2114-2125 Chen, G., Yin, K., Shi, L., Fang, Y., Qi, Y., Li, P., Luo, J., He, B., Liu, M., and Shi, T (2011) Comparative analysis of human protein-coding ... (2010) Stage-specific role for shh in dopaminergic differentiation of human embryonic stem cells induced by stromal cells Stem Cells Dev 19, 71-82 Takahashi, K., Tanabe, K., Ohnuki, M., Narita,...
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Human embryonic stem cell derivatives as cancer therapeutics
Ngày tải lên :
09/09/2015, 18:52
... factor Human embryonic stem cell Human embryonic stem cell derived dendritic cell Human embryonic stem cell derived mesenchymal stem cell Human embryonic stem cell derived neural stem cell Hematopoietic ... dendritic cell Myeloid progenitor cells Mesenchymal stem cell Natural killer T cell Neural stem cell Poly(2-hydroxyethylmethacrylate) Protein phosphatase 1, regulatory subunit 12C Pluripotent stem cell ... observed 1.2 Adult stem cells and cancer therapy Stem cells, by definition, possess two main characteristics Stem cells are able to produce daughter cells that retain their stem cell potential (selfrenewal),...
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Human embryonic stem cell derived neural stem cells derivation, differentiation and MicroRNA regulation
Ngày tải lên :
10/09/2015, 09:08
... NSC differentiation 1.1.5.2 Pluripotent stem cell- derived NSCs Human embryonic stem cells (hESCs), which are pluripotent cells derived from the inner cell mass of blastocyst-stage embryos, offer ... of Human Embryonic Stem Cells in mTeSRTM1” (STEMCELL Technologies Inc.) In brief, H1 hESCs were maintained on BD matrigel hESC-qualified matrix (BD Biosciences) with mTeSRTM1 medium (STEMCELL ... HUMAN EMBRYONIC STEM CELL- DERIVED NEURAL STEM CELLS: DERIVATION, DIFFERENTIATION AND MICRORNA REGULATION KWANG WEI XIN...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 1
Ngày tải lên :
11/09/2015, 10:06
... costeffective culture systems In view of the imperfections of existing culture systems, an optimal system for the culture and derivation of hESCs would require feeder-free culture on a human ECM, ... entire co -culture population, therefore adding significantly to background noise Even the use of human cells as feeders poses challenges for hESC culture, as the dependence on a co -culture system ... DxSDOCDOC matrices and cultured using defined medium mTeSR-1 (StemCell Technologies, 05850) Culture medium was changed daily and subculture was done every 5-7 days 2.1.3 iPSCs DL1 cells were generously...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 2
Ngày tải lên :
11/09/2015, 10:06
... Isoform of 100.00% 3.33% 47 IPI00220967, IPI00744135, IPI00856098 IPI00009865 58811 IPI00220327 Extracellular Matrix Ribosome-binding protein 66022 IPI00297646 138923 IPI00304962 129297 IPI00013079 ... GFR and standard Matrigel, based on work by Hughes et al 2009 † Murine protein is a homolog of human protein 50 ...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 3
Ngày tải lên :
11/09/2015, 10:06
... maintained using commercially available defined culture medium, mTeSR-1, which has been optimized for Matrigel, the standard feeder-free culture system Subculture was done every 5-7 days depending ... and DxSDOCDOC matrices hESCs were subcultured onto either DxSDOC or DxSDOCDOC matrices using collagenase IV for enzymatic passaging Control hESCs were subcultured onto Matrigel-coated wells using ... hESCs did not adhere to DxSNP40/DNase matrices, and settled on the bottom of the culture well as clusters of cells that were easily moved by mechanical disturbances to the plate (Figure 4B) Likewise,...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 4
Ngày tải lên :
11/09/2015, 10:07
... is a sign of the aging of the cells hESCs on feeder-free culture systems tend to acquire senescence at earlier passages compared to those on feeder co -culture systems, and senescence is usually ... MEFconditioned medium [4] According to mTeSR-1’s manufacturers, StemCell Technologies, hESCs can be cultured on Matrigel in the defined culture medium for up to 18 passages [23] When proliferation ... 34.6% of hESCs cultured on Matrigel, 36.8% of hESCs cultured on DxSDOC and 37.2% of hESCs cultured on DxSDOCDOC were positive for TRA-1-60 Conversely, 77.7% and 75.1% of hESCs cultured on Matrigel...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 5
Ngày tải lên :
11/09/2015, 10:07
... differentiation hESCs cultured on DxSDOCDOC for 13 passages were induced into neural differentiation using a protocol modified from WiCell Research Institute, National Stem Cell Bank In this protocol, ... regulating cellular differentiation, and because little is known about the epigenetic factors that define the differentiation state or potency of stem cells in comparison to differentiated cells, ... propagation Once again, hESCs were cultured onto Matrigel, DxSDOC and DxSDOCDOC matrices for up to 20 passages hESCs were maintained in defined culture medium, mTeSR-1, and subcultured every 5-7 days depending...
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n vitro bioassembled human extracellular matrix and its application in human embryonic stem cell cultivation 6
Ngày tải lên :
11/09/2015, 10:07
... made at each human embryonic stem cell culture step that can contribute to a safer and more efficient human embryonic stem cell culture: For the enhancement of collagenous extracellular matrix, ... developing an alternative embryonic stem cell culture system, with the aim of overcoming current obstacles that hinder the safe clinical implementation of human embryonic stem cell culture technologies ... Feeder layerand serum-free cultured of human embryonic stem cells Biol Reprod 70: 837-45 (2004) 26 Amit M and Itskovitz-Eldor J Feeder-free culture of human embryonic stem cells Methods Enz 420:38-49...
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A human embryonic stem cell based model of trophoblast formation
Ngày tải lên :
11/09/2015, 16:07
... METHODS 2.1 Cell culture 2.1.1 Mouse embryonic fibroblasts (MEFs) 54 56 57 57 2.1.1.1 Derivation of MEFs 57 2.1.1.2 Culture of primary MEF 58 2.1.2 Mouse ES cell culture 58 2.1.3 Human ES cell culture ... function of immune cells 20 1.2.3.5 Other placenta related factors 21 iv 1.2.4 Clinical relevance of placenta development 1.3 Embryonic stem cells 1.3.1 Mouse embryonic stem cells 22 23 23 1.3.1.1 ... Introductory statement Embryonic stem cells (ESCs) are derived from the inner cell mass (ICM) of the preimplantation embryo ESCs are pluripotent stem cells as, under well established culture conditions,...
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Báo cáo y học: "A mouse embryonic stem cell bank for inducible overexpression of human chromosome 21 genes." ppsx
Ngày tải lên :
09/08/2014, 20:22
... spreads were analyzed to assess the percentage of euploid cells Embryonic stem cell differentiation The EB3 cells and the parental line E14 cells [43] were allowed to differentiate using the 'hanging ... colonization by cultured cells Nature 1987, 326:292-295 Wobus AM, Guan K, Yang HT, Boheler KR: Embryonic stem cells as a model to study cardiac, skeletal muscle, and vascular smooth muscle cell differentiation ... pluripotency and embryonic stem cell fate in Down syndrome Am J Hum Genet 2008, 83:388-400 19 Masui S, Shimosato D, Toyooka Y, Yagi R, Takahashi K, Niwa H: An efficient system to establish multiple embryonic...
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Establishment of autologous culture systems for human embryonic stem cells
Ngày tải lên :
11/09/2015, 10:00
... sources, named embryonic stem cells Literature review (ESCs), embryonic germ cells, fetal stem cells, umbilical cord stem cells, adult stem cells and induced pluripotent stem (iPS) cells (Ariff ... pluripotent cell source for future clinical applications Literature review Table Characterization of stem cells by derivation source Category Embryonic stem cells Embryonic germ cells Fetal stem cells ... 2007) Lymphoid stem cells, Myeloid stem cells Unipotent Retain self-renewal capacity but can only produce one cell type (Sorgner, 2007) Muscle stem cells Apart from the plasticity, stem cells can...
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