protein and peptide analysis by mass spectrometry
protein and peptide analysis by mass spectrometry
... L., and McLuckey, S. A. (1988) Mass Spectrometry /Mass Spectrometry Technzques and Applications of Tandem Mass Spectrometry VCH, New York. 16. Hogeland, K. E , Jr and Demzer, M L (1994) Mass ... 1 Mass Spectrometry in the Analysis of Peptides and Proteins, Past and Present Peter Roepstorff When the editor, John Chapman, asked me to write the introductory chapter to this volume and ... liquid chromatography mass spectrometry Part II Techniques and mechanisms of thermospray Mass Spectrom Rev 9,63 l&669 9 Dawson, P H (1976) Quadrupole Mass Spectrometry and Its Applrcatlons...
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Báo cáo khoa học: A mutagenic analysis of the RNase mechanism of the bacterial Kid toxin by mass spectrometry pptx
... binding (T46, A55, T69 and R85) or RNAcleavage (R73, D75 and H17) were analysed by mass spectrometry in RNAbinding and cleavage assays. A pair of substrates, 5¢-AUACA-3¢, and itsuncleavable mimetic ... residues of the protein and by analy-sing in vitro their effects on RNA binding and RNAcleavage using short RNA substrates and native MSassays. Our analysis focuses on the protein residuesproposed ... 159-06) to visit and work at the Bio-molecular Mass Spectrometry and Proteomics groupat Utrecht University, the Netherlands. The technicalassistance of Alicia Rodriguez-Bernabe´ and discus-sions...
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Tài liệu Báo cáo khoa học: A functional polymorphism of apolipoprotein C1 detected by mass spectrometry docx
... dithio-threitol and iodoacetamide, with quantification of Cys by detection of mass change of a peptide after reduc-tion and alkylation. These and other approaches havebeen described previously [19], and ... protease, and the pep-tides subjected to MS. The peptide mass fingerprintfrom MALDI-TOF MS showed m ⁄ z values corres-ponding to all eight theoretical peptides plus the peptide of the truncated protein ... the peptides from the gel wereaccomplished by standard procedures. Peptide fingerprintmaps of the digested proteins were obtained in both theBruker Biflex III operating in the reflectron mode and...
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Tài liệu Báo cáo khoa học: Si-face stereospecificity at C5 of coenzyme F420 for F420H2 oxidase from methanogenic Archaea as determined by mass spectrometry ppt
... [14a-1H2]methylene-H4MPTwas prepared by spontaneous reaction of H4MPT and 1H2CO and [14a-2H2]methylene-H4MPT by spontaneousreaction of H4MPT and 2H2CO [30]. FprA from M. marbur-gensis [20] and Mtd from ... datawere carried out in excel 2000 and transformed into stickspectra separated by 1 Da [31].AcknowledgementsThis work was supported by the Max Planck Society and by the Fonds der Chemischen Industrie. ... oxidation of 2 F420H2withO2to 2 F420 and 2 H2O. The 45 kDa protein contains1 FMN per mol and harbours a binuclear iron centreindicated by the sequence motif H-X-E-X-D-X63-H-X18-D-X62-H....
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basic protein and peptide protocols
... two alternative protein standards, bovine serum albumin and ovalbumin, are 0.66 and 0.75, respectively. 3. Plastic and glassware used in the assay should be absolutely clean and detergent-free. ... the Coomassie blue protein assay to different proteins. Anal. Btochem. 184, 111-I 16. 6. Spector, T. (1978) Refinement of the Coomassie blue method of protein quan- titation. A simple and linear ... 4. Standards: Use a stock solution of standard protein (e.g., bovine serum albumin fraction V) containing 4 mg/mL protein in distilled water stored frozen at -2OOC. Prepare standards by diluting...
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Tài liệu Báo cáo khóa học: Quantitative analysis, using MALDI-TOF mass spectrometry, of the N-terminal hydrolysis and cyclization reactions of the activation 2 process of onconase pdf
... overall peptide function, as has been shown by the hypothalamic releasingfactor binding to its receptor [2], or by the amyloid b -peptide and the implications in senile plaque formation and pathogenesis ... wereincubated at 37 °C, 50 °Cand70°C, respectively. Fromthese, aliquots of 2 lL were taken at 0, 1, 2, 4, 6 and 24 h, and prepared for MS analysis as described above9.MALDI-TOF MSAll mass spectra were ... was adjusted to 2.5, 8.0 and 11.5 and the samples were incubated at 37 °C, 50 °Cand70 °C. Aliquots were taken at different time-points fromeach sample and analyzed by MALDI-TOF MS. Theaddition...
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Tài liệu Báo cáo khóa học: Determination by electrospray mass spectrometry and 1H-NMR spectroscopy of primary structures of variously fucosylated neutral oligosaccharides based on the iso-lacto-N-octaose core doc
... oligosaccharides, were initially elucidated by high-sensitivity ES-MS/MS analysis, and then their full structureassignment was completed by methylation analysis and 1H-NMR. Three new structures were ... hepatitisB and HIV tests. Written consent was obtained from thisvolunteer for analysis of the milk sample. Fat wasremoved by centrifugation at 4 °C (5000 g,30min )and proteins by precipitation ... deter-mined by high-sensitivity ES-MS/MS analysis as demon-strated here and also previously [13,14]. Even a minorisomeric component present in a mixture can be detected,as exemplified by the analysis...
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Tài liệu Báo cáo khoa học: The resident endoplasmic reticulum protein, BAP31, associates with c-actin and myosin B heavy chain Analysis by capillary liquid chromatography microelectrospray tandem MS ppt
... 33 kDa and 28 kDa,respectively, and two additional protein bands at apparentmasses of 42 and 190 kDa [9]. For sequence analysis, theBAP31 immunoprecipitation protocol was scaled up and the ... characterizationwas achieved by manually excluding tandem mass spectra ofpoor quality, by restricting the database to the proteins thatwere identified in a first pass approach, and by changing theSEQUESTparameter ... aminomethane, 385 mMglycine, 0.2% (w/v) SDS]. Protein bands were visualized by Coomassie blue staining.Bands of interest were excised from the gel and proteinswere digested in-gel following a published...
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TECHNIQUES FOR THE ANALYSIS OF ORGANIC CHEMECALS BY INDUCTIVELY COUPLED PLASMA MASS SPECTROMETRY (ICP-MS) pptx
... removal and reproducibility of the system. Figure 4. Cool plasma analysis calibrations in IPA. Standard addition at 0, 5, 10 and 20 ppt, showing effective removal of 12C2 and 40Ar12C ... Coupled Plasma Mass Spectrometry (ICP-MS) is used for the routine monitoring of trace and ultra-trace metal contaminants in aqueous-based chemicals. Recent advances in ICP-MS technology and methodology ... solvent. Direct organic solvent analysis demands some specific features and capabilities of the ICP-MS instrument, particularly in the sample introduction and plasma systems. Details are...
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Báo cáo khoa học: A region within the C-terminal domain of Ure2p is shown to interact with the molecular chaperone Ssa1p by the use of cross-linkers and mass spectrometry doc
... d4(B) is observed. The peptides indicated by arrowheads wereidentified by exact mass measurement. The peptide labeled by curved arrows was identified by tandem mass spectrometry as amono-linked ... Chemical cross-linking and mass spec-trometry to map three-dimensional protein structures and protein- protein interactions. Mass Spectrom Rev25, 663–682.10 Lutter LC & Kurland CG (1975) Chemical ... mass spectrometry for protein interaction analysis: application to a calmodu-lin ⁄ target peptide complex. Anal Chem 77, 495–503.19 Tang XT, Munske GR, Siems WF & Bruce JE (2005) Mass spectrometry...
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