... suitable for routine monitoring or measurement of a large number of samples, and lead to better understanding of the contribution of Candidatus ‘Accumulibacter phosphatis’ to phosphorus removal in ... conditions included a pre-incubation step of 5 min at 94 °C followed by 35 cycles of 94 °C for 15 sec, 57 °C for 20 sec, and 68 °C for 15 sec. Journal of Water and Environment Technology, Vol.5, ... (BIO 101, Q-BIO gene), in accordance with the instructions provided by the manufacturer. Concentration of DNA was determined using a NanoDrop ND-1000 spectrophotometer (Nanodrop Technologies)....