methylmeter r a quantitative sensitive and bisulfite free method for analysis of dna methylation

báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis" potx

Ngày tải lên : 12/08/2014, 03:20

... the reaction mixture and boiled for Proteins were separated by SDS-PAGE and transferred to Hybond-LFP PVDF membrane (GE Healthcare) according to standard procedures Immunoblot analysis was carried ... collected by centrifugation using a Hitachi R1 0H rotor Each mRNA (usually 30–35 μg) was washed and transferred into a translation mixture The translation reaction was performed in the bilayer mode [35] ... carried out with 100 nM SPu primer and 100 nM AODA2303 primer and used as transcription templates Cell -free Protein Synthesis In vitro transcription and cell -free protein synthesis were performed...

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Báo cáo khoa học: A new and efﬁcient method for inhibition of RNA viruses by DNA interference pptx

Ngày tải lên : 16/03/2014, 02:20

... (5¢-ATGAGTTTTCCAGAGCAACTT-3¢), and siRNA was synthesized using DNA templates (sense strand, 5¢-AAATGAGTTTTCCAGAGCAACCCTGTCTC-3¢; antisense strand, 5¢-AAGTTGCTCTGGAAAACTCATCCTG TCTC-3¢) and a Silencer siRNA ... higher concentrations of siDNAs than of siRNAs is necessitated by the lower stability of RNA DNA than of RNAÆRNA duplexes formed within RISC On the other hand, DNA oligomers are more resistant ... the antiviral effects of short and long DNA and RNA molecules speciﬁc for TMV genomic RNA We have found that both long dsRNA and long dsDNA are potent inhibitors of viral RNA A long dsDNA fragment...

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A combined statistical and in silico framework for analysis and characterization of microbial and mammalian metabolic networks

Ngày tải lên : 11/09/2015, 21:27

... is also a need for normalizing the data as different scales and units may lead to irrelevant or insignificant results from data analysis Thus data sets are generally scaled to mean and standard ... Tools for multivariate data analyses In this part a comprehensive review of the available data preprocessing and analysis tools for biological processes are summarized Often datasets obtained from ... scores The overall characteristics, presence of outliers and clusters are explored using scores plots, while correlated variables and important variables that contribute to major variations are...

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Báo cáo y học: "A comparative analysis of DNA methylation across human embryonic stem cell lines" docx

Ngày tải lên : 09/08/2014, 23:20

... X, Rigor P, Baldi P: MotifMap: a human genome-wide map of candidate regulatory motif sites Bioinformatics 2009, 25:167-174 Yoshida-Hata N, Mitamura Y, Oshitari T, Namekata K, Harada C, Harada ... Irizarry RA, Ladd-Acosta C, Carvalho B, Wu H, Brandenburg SA, Jeddeloh JA, Wen B, Feinberg AP: Comprehensive high-throughput arrays for relative methylation (CHARM) Genome Res 2008, 18:780-790 Rollins ... mismatches are allowed in our analysis It is the only aligner that is able to handle reads generated from different library protocols using pre-methylated adapters (H1, H9), or the Dpn1 adapter...

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3 carbon isotope discrimination in roots and shoots of major weed species of southern u s rice fields and its potential use for analysis of rice–weed root interactions

Ngày tải lên : 04/09/2015, 07:24

... by Amazon sprangletop, intermediate in broadleaf signalgrass, fall panicum, and barnyardgrass, and greatest in crabgrass and yellow nutsedge (210.3%) (Table 2) Rajagopalan et al (1999) reported ... of roots of weedsuppressive rice and barnyardgrass in soil (Gealy and Fischer 2010) Barnyardgrass is an aggressive tropical grass that greatly affects rice production worldwide 13C is a naturally ... where d13Csample is the isotope ratio (in parts per thousand; %) relative to the PDB standard Rsample and Rstandard are the 13 12 C/ C molar abundance ratios of the plant sample and the PDB standard...

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Development and application of novel capillary electrophoresis techniques for analysis of DNA fragments and organic pollutants

Ngày tải lên : 14/09/2015, 11:40

... potential for on-site analysis of various pollutants at trace level CE is found to be a versatile analytical tool for the analysis of DNA as well as pollutants Combination of DNA analysis and environmental ... XII Summary Summary This dissertation is broadly divided into parts: the first part (chapter and chapter 3) focused on the analysis of DNA fragments; and the second part (chapter and chapter 5) ... where σ is the total spatial variance of the concentration profile for a zone If the peaks acquired are symmetrical and have Gaussian profile, the theoretical plate number can also be calculated...

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Báo cáo khoa học: Cleavage site analysis of a serralysin-like protease, PrtA, from an insect pathogen Photorhabdus luminescens and development of a highly sensitive and speciﬁc substrate pdf

Ngày tải lên : 23/03/2014, 09:20

... metallo-endoprotease of Serratia marcescens (serralysin), are the alkaline proteinase of Pseudomonas aeruginosa, the ZapA metalloprotease of Proteus mirabilis and proetases A, B, C, G and W of ... quencher group absorbs For comparison of hydrolysis rates, 0.25 mm furylacryloylLGPA was added to the hydrolysis reactions as an internal standard, because it was not hydrolysed by PrtA Peak areas ... position of cleavage sites (vertical arrows, a1 a3 ) and cleavage fragments (horizontal double arrows, A1 A5 ) in the sequence of insulin chain A (B) Change over time in the chromatographic peak area of...

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Báo cáo khoa hoc:" Quantitative competitive reverse transcription polymerase chain reaction is not a useful method for quantification of CD4 and CD8 cell status during HIV infection" pptx

Ngày tải lên : 11/08/2014, 08:20

... smaller amounts of blood, such as that from a heelstick or finger-prick [2] Quantitative methods for reverse transcriptase polymerase chain reaction (RT-PCR) are rapidly surpassing all other methods ... order to avoid amplification of cellular DNA contaminating RNA preparations Products are generated from cDNA and deletional mutants are constructed by restriction endonuclease digestion and religation ... GAPDH The success of the RNA extraction and reverse transcription is determined by quantification of GAPDH mRNA by QC-RT-PCR Each value for quantity of CD4 or CD8 mRNA was standardized according...

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Tài liệu Báo cáo khoa học: Application of a ﬂuorescent cobalamin analogue for analysis of the binding kinetics A study employing recombinant human transcobalamin and intrinsic factor pdf

Ngày tải lên : 19/02/2014, 05:20

... TC puried from plants [17] and yeast [18], respectively Both proteins were originally obtained as Cbl-saturated holo-forms, and preparation of the unsaturated apo-forms required their denaturing ... Pi buffer, pH 7.5, 20 C was incubated over time Free ligands were adsorbed on charcoal, and the absorbance spectra were recorded Concentration of appearing IFCNCbl was calculated by comparison ... Applicability of CBC as a tool for analysis of the binding kinetics was established and allowed to make several inferences concerning the proteinligand and proteinreceptor interactions Furthermore, our results...

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Báo cáo khoa học: "A Knowledge-free Method for Capitalized Word Disambiguation" doc

Ngày tải lên : 17/03/2014, 07:20

... capitalized words are particularly problematic: some capitalized words are proper nouns and some are not Estimates from the Brown Corpus can be misleading For example, the capitalized word "Acts" ... Stochastic parts program and noun-phrase parser for unrestricted text In Pro- ceedings of the Second A CL Conference on Applied Natural Language Processing (ANLP'88), Austin, Texas W Francis and ... can capture that in the first case the word "All" modified a singular proper noun ("Bank") and hence is not grammatical as an external modifier and in the second case it is a grammatical external...

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An application of GIS and Remote Sensing for Analysis of Agricultural Development-Induced Changes in Land Use: A case study in Lao PDR pdf

Ngày tải lên : 17/03/2014, 11:20

... (decreased/increased) and fragmented (field crop, evergreen forest, fallow forest) as a result of both farmers who lost their lands and turned to clear-cut forest areas for upland rice cultivation, ... the area, and than this place was changed in dynamics way of land use system • The forest area was destroyed by increasingly shifting cultivation and rubber plantation areas • Lack of an appropriate ... data, GIS data and satellite imagery data) • The zonation can be regarded as a tool for sustainable agricultural development in the watershed area 17 Suggestion zones for sustainable of watershed...

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báo cáo hóa học: " In vitro and in vivo pre-clinical analysis of a F(ab’)2 fragment of panitumumab for molecular imaging and therapy of HER1-positive cancers" pot

Ngày tải lên : 21/06/2014, 02:20

... vitro and in vivo characterization of panitumumab F(ab’) fragment with an emphasis on its evaluation towards both imaging and therapeutic applications Materials and methods Preparation of F(ab’)2 ... wet-weighed, and the radioactivity measured The values represent the average percent injected dose per gram (%ID/g) of tissue along and the standard deviations xenograft targeting was performed The targeting ... Quantitation of tumor and normal organ distribution of Y-CHX -A -panitumumab F(ab’)2 (a) Receptor-mediated uptake of 86YCHX -A" -panitumumab F(ab’)2 of LS-174T tumor xenografts and normal organs...

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Báo cáo hóa học: " Research Article A Low-Complexity LMMSE Channel Estimation Method for OFDM-Based Cooperative Diversity Systems with Multiple Amplify-and-Forward Relays" docx

Ngày tải lên : 22/06/2014, 00:20

... eﬀect of the number of relays on the MSE and bit error rate (BER) performance alone, these relays are assumed to be distributed in a symmetrical way, for example, the geometric gains GSRi and GRiD ... noise (AWGN) at terminal Ri with zero-mean and variance σn As performing DSTC in the data transmission section, terminal Ri is also assumed to forward a linear function of its received signal vector ... the case of larger GSRi Figure 4: BER performance comparison of the proposed channel estimation method using diﬀerent training sequences 5.3 relays provide better BER performance than two relays...

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Báo cáo nghiên cứu khoa học: " TOTAL POLYPHENOLS, TOTAL CATECHINS CONTENT AND DPPH FREE RADICAL SCAVENGER ACTIVITY OF SEVERAL TYPES OF VIETNAM COMMERCIAL GREEN TEA" pdf

Ngày tải lên : 22/07/2014, 06:20

... foods and grape seed oil, Journal of health science 49, p 45 54, (2003) [9] Elena N Hristea, Mihaela Hillebrand, Miron T Caproiu, Horia Caldararu, Titus Constantinescu, Alexandru T Balaban, Scavenging ... flavonoids with iron and copper ions: A mechanism for their antioxidant activity, Free radical research, p 1-10, (2002) [11] S Gupta, B Saha, A. K Giri, Comparative antimutagenic and anticlastogenic ... area and the tea industry capacity [1] But quality assurance in tea process is still inadequate and out of date Therefore it is necessary to carry out surveys to determine TPP, TCT and RSC of available...

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Báo cáo toán học: "EMILION, a tree functional-structural model: Presentation and first application to the analysis of branch carbon balance" pdf

Ngày tải lên : 08/08/2014, 14:22

... carbon balance CBY of each branch, and the average for all branches The average behaviour of branches was characterised by (1) a small deficit in carbon fixation during the first year (–1.1 mol ... photosynthetic parameters Vcmax, Jmax, α, and Rd as a function of needle age Age (year) and needle temperature T (°C) Adapted from Porté and Loustau [18] Photosynthetic parameters and units Vcmax Jmax α Rd ... the spatial and temporal variability of these characteristics must be coupled to the analysis of processes studied and parameterised at a level lower than the tree, in order to obtain a reliable...

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Báo cáo khoa học: "The study of tree fine root distribution and dynamics using a combined trench and observation window method" doc

Ngày tải lên : 09/08/2014, 02:21

... various observation methods and techniques are discussed of A trench and an observation window tested in order to estimate root distribution and growth in a natural oak-birch stand in central ... concerning root observation and biometrics, J Roque (INRA - SESCPF) for the soil description, M Bariteau, L Bouvarel and the technical staff of the Orl!ans INRA Sylviculture and Biomass laboratories ... structural roots accounting for the major part of the underground biomass Above-ground biomass amounts here to = 80-100 t.ha-! (Auclair and Metayer, 1980) The underground parts we have measured represent...

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Báo cáo y học: "A functional difficulty and functional pain instrument for hip and knee osteoarthritis" ppsx

Ngày tải lên : 09/08/2014, 14:22

... calibrations for hip and knee patients and for males and females and incorporate them into future CAT applications This is an approach that we consider interesting for potential future research ... of the ability ranges for pain and function The program fed the response to the first item into the CAT algorithm and calculated a probable score and person-specific standard error (measure of ... following characteristics in our analysis: accuracy, breadth of coverage, reliability, and precision We assessed the accuracy of CATs relative to the full item bank by calculating Pearson correlation...

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Báo cáo y học: "ExpressionPlot: a web-based framework for analysis of RNA-Seq and microarray gene expression data" doc

Ngày tải lên : 09/08/2014, 23:20

... gene expression data Brad A Friedman1,2,3* and Tom Maniatis4 Abstract RNA-Seq and microarray platforms have emerged as important tools for detecting changes in gene expression and RNA processing ... be part of another project, and maybe even have been performed on another platform (for example, RNA-Seq versus microarray) or in another organism (for example, human versus mouse) The 4way tool ... the Barres Lab (Stanford), Myers Lab (HudsonAlpha Institute), Ravits Lab (Benaroya Institute) and Maniatis Lab (Harvard/Columbia) for providing data and/ or user feedback This work was supported...

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Báo cáo y học: "Development of a Highly Sensitive Method for Detection of JAK2V617F" docx

Ngày tải lên : 10/08/2014, 21:23

... cause vascular damage and thereby trigger heart disease In any case, relevance of JAK2V617F positivity with cardiovascular disorders warrants further investigations Discovery of JAK2V617F represents ... JAK2V617F mutation by asymmetric PCR and melt curve analysis Cancer Biomark 2007, 3:315-324 23 Rapado I, Albizua E, Ayala R, Hernández JA, Garcia-Alonso L, Grande S, Gallardo M, Gilsanz F, Martinez-Lopez ... Pardanani A, Tefferi A: Clinical correlates of JAK2V617F presence or allele burden in myeloproliferative neoplasms: a critical reappraisal Leukemia 2008, 22:1299-1307 36 Pardanani A, Tefferi A: ...

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báo cáo khoa học: " Surface electromyography as a screening method for evaluation of dysphagia and odynophagia" ppt

Ngày tải lên : 11/08/2014, 20:20

... clinically important as an additional tool for establishing aetiology and localization – oral, pharyngeal, or oesophageal – of causes for dysphagia or odynophagia Each stage has its mean normal duration ... given: "Swallow your saliva" Three trials of swallowing normal volume of tap water for a particular person These volumes, calculated for a particular age group [31-36], are: Group (age 18–40) ... person per procedure is a combination of the time needed for preparation of a patient, time needed for preparation of an instrument, actual time of a procedure, and the time needed for data interpretation...

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