... ChinaFax: +86 22 23542503Tel: +86 22 23542503E-mail: htang2002@yahoo.com*These authors contributed equally to thiswork(Received 11 April 2010, revised 15 June2010, accepted 12 July 2010)doi:10.1111/j.1742-4658.2010.07773.xMicroRNAs ... viaPCRs. PCR cycles were as follows: 94 ° C for 4 min, followedby 40 cycles of 94 °C for 1 min, 56 °C for 1 min and 72 °Cfor 1 min. PCR primers were as follows: IL6R sense,5¢-CCGAGATCTGGCTTTTACTTAAACCG-3¢; ... Berlin,Germany. http://pictar.mdc- berlin.de/ cgi-bin/new_PicTar_vertebrate.cgi) and TargetScan Release 5.1 (Whitehead Insti-tute for Biomedical Research, Cambridge, MA, USA, http://www.targetscan.org).EGFP...