... snake. 14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply the correct formofthe verbs1. Cats could fly if they (have) wings.2. If Peter (study) harder, ... Cats could fly if they (have) had wings.2. If Peter (study) studied harder, he would get better marks.3. You will be late for class if you (not, hurry) don't hurry 4. Mary would not have ... better marks.3. You will be late for class if you (not, hurry). 4. Mary would not have got wet if she (wear) a raincoat.5. If today (be) a holiday, I would stay in bed for all day long.6. If...
... effectively digested by intracellular proteases.Contributions ofthe carbohydrate moiety The specific activities of glycosylated and unglycosylatedCPY are the same as previously reported [13] with the ... higher by 4 °Cthanthat of Dgly proCPY, indicating that the carbohydratemoiety exerts a slightly protective effect on the thermalunfolding of proCPY. The Pmvalue for CPY was higher than that of ... itsunglycosylated form, though the DV and DGPvalues werealmost the same. The Pmvalue of proCPY was higher thanthat of its unglycosylated form. This is due to the higher DVvalue ofthe unglycosylated...
... confirmed the role of LHBs in the HBV attachment [23]. Recently, the attachment site of LHBs was functionally narrowed down to the amino acids 21 47 of preS1 by employing synthetic peptides. The ... translocation ofthe internally sequence of PreS1 domain onto the viral surface [62, 63]. This supports the hypothesis that the low pH in the endosome may induce the conformation change of HBV surface ... by the observation ofthe conformation change ofthe large surface protein of DHBV at low pH condition [62]. However, the more detail investigation was from the kinetic study ofthe uptake of...
... - 2,9 6 ,4 - 6,8 4, 3 - 2,6 2 40 M 50 50 Defect at 3 walls 35 - 36 7,5 - 5,0 4, 3 - 3,8 6 - 6 ,4 3,3 - 1,3 3 40 M 50 50 Defect at 3 walls 44 - 45 7,5 - 5,0 4, 3 - 3,1 4 - 4, 3 3,3 - 1,9 4 28 M 60 ... ophthalmology, dermatology, dentistry and rheumatology [10,11]. Based up on these data the aim of this study was to observe the potential of Esterified Hyaluronic Acid (EHA) as a coadjuvant of grafting ... Department of Dental Sciences and Surgery, University of Bari, Bari, Italy. Correspondence to: Prof. F.R. GRASSI, Professor and Dean, Department of Dental Sciences and Surgery - University of Bari,...
... FEBSnon-covalent complex ofthe pro-peptide and BMP-9can bind to the type I receptor Alk1 [17], the latency-associated polypeptide of TGFb inhibits the interac-tion of TGFb isoforms with type II and III ... phosphorylation. The data presented here suggest that the pro-domain of BMP-2 can alter the signalling properties ofthe growth factorby modulating the ability ofthe mature part to interact with the ... transforminggrowth factor b (TGFb) ⁄ BLP superfamily. The role ofthe 263 amino acids of the pro-peptide is currently unclear. In order to obtain an insight into the function ofthe pro-form...
... rearranged in the dTTP complex to accommodate the 5-methylgroup ofthe thymine moiety. As a result, the Ala1 24 carbonyl was moved from the 4- oxo ⁄ 4- amino group of the bound nucleotide and the side ... DenmarkSynthesis of dTMP by thymidylate synthase proceedsby the reductive methylation of dUMP, which isobtained via one of two parallel pathways. One path-way, considered to be a minor supplier of ... after the transition ofthe enzyme to amore active form, respectively, t is the time and s is the lag-time. The rate constant, k, for the activation ofthe enzyme isobtained as 1 ⁄ s.CrystallizationCrystals...
... whether each tran-script possesses the activity to support activation of the Nox enzymes. In this study, we show the expres-sion of alternatively spliced transcripts ofthe NOXO1gene, by PCR ... 2C, the mRNA forNoxo1c was expressed substantially in the testis butonly slightly in the colon. On the other hand, the Noxo1b mRNA was relatively abundant in the colonand also present in the ... bygp91phoxleads to subsequent formation of microbicidalreactive oxygen species such as hydroxyl radical andhypochlorous acid. Nox1, the second member of the Nox family, is abundant in the...
... for the first time the results of acomparative study on the organic matrix extracted from the nacreous layer ofthe shell from the pearl oysterPinctada maxima by two very different methods. The ... 7 54. 46 .Marxen,J.C.,Hammer,M.,Gehrke,T.&Becker,W.(1998)Carbohydrates ofthe organic shell matrix and the shell formingtissue ofthe snail Biomphalaria glabrata (Say). Biol. Bull. 1 94, 231– 240 . 47 . Miyazawa, T. & Blout, E.R. (1961) The infrared ... aWaters 42 0 fluorimeter. Proline, hydroxyproline andhydroxylysine were examined at 2 54 nm by reverse-phaseHPLC of their phenylisothiocarbamate derivatives [26], asreported previously [20]. The...
... [ 54] .As th e activity of this mutant enzyme could not be restoredby the addition of Tween 40 [39], i t can be concluded that the 48 2 Gly residue is important for the activity of the enzyme, ... via three enzymatic steps carried out by the enzymes phytoene synthase, phytoene dehydrogenase andlycopene cyclase. The enzyme phytoene dehydrogenase isable to introduce four dehydrogenations ... while the catalytic activity ofthe phytoene dehydrogenase in strainS 442 is directly affected by the mutation, strain C5 possessesa f unctional enzyme, likely altered in a region relevant forthe...
... L 4 –5¢(Gln4 34- Arg 441 ).These two loops interact with the tips of four otherloops: L1–1¢(Gly378-Asp375), L2¢–2(Ala402-Arg4 04) ,L3¢ 4 (Gly4 24- Gly427) and L6–6¢(Glu498-Gly500). The buried surface area ofthe ... KGKYHMFYQYNPRKPEWG-NICWGHA 1Y4 W FNYDQ-PYRGQYHFSPQKNWMNDPNGLLYH NGTYHLFFQYNPGGIEWG-NISWGHA3KF5 SIDLSVDTSEYNRPLIHFTPEKGWMNDPNGLFYDKTAKLWHLYFQYNPNATAWGQPLYWGHA2AC1 NQ-PYRTGFHFQPPKNWMNDPNGPMIY KGIYHLFYQWNPKGAVWG-NIVWAHS2ADE ... states(Fig. 4) . In the first step ofthe enzymatic reaction, anucleophilic attack is performed on the anomeric car-bon ofthe sugar substrate by the carboxylate of the Asp 54 acting as the primary nucleophile,...
... by carrying out the labelling procedure withoutprimary antibody. The efficiency of blocking was controlledby performing the labelling procedure in the absence of the second primary antibody. ... University of Joensuu, Finland3 Biocenter Oulu and Department of Pathology, University of Oulu, Finland 4 National University of Ireland, Department of Biochemistry, Cell Cycle Control Laboratory, ... hyperphosphorylatedelongating isoform RNA pol IIORNA pol II exists in a dynamic equilibrium between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO thatexhibits...
... [15].Assay of human PKIb activity The activity of purified human PKIb was assayed by the inhibition ofthe catalytic subunit of cAPK in a 50 lLreaction containing 0.5 units of purified catalytic subunit,25 ... acetate buffer. The pooledfraction was stored at )20 °C for further study. The concentration ofthe proteins was determined by the method of Lowry et al. [ 14] and the purity was examinedby SDS/PAGE ... showedasinglebandbySDS/PAGE(Fig. 2). The assay of its activity demonstrated that the purified PKIb inhibited the catalytic subunit of cAPK with the specific activity of 6.0 · 10 4 unitÆmg)1(Fig. 3A) .The Kivalue...
... fatty acid elongation,in contrast to the multifunctional enzyme catalyzing all the steps ofthe type I FAS pathway [4, 5]. ACP is anessential component of both type I and type II fattyacid synthesis ... apparent from the solution denaturation studiesthat the holo formofthe protein has greater stabilitythan the apo form. The differences in the unfoldingthermodynamic parameters ofthe two forms are ... done. The higher stabil-ity ofthe holo form can be attributed to the number of favorable contactsthat the4 -phosphopantetheine group makes with the surface residues byvirtue of a number of hydrogen...
... mM of S-decyl-glutathione, the meas-ured ATPase activity represented approximately the basalATPase activity. This type of behaviour was also observedfor the stimulatory effect of S-decyl-glutathione ... concentrations were determinedby the Bradford assay using BSA as the standard. The following purified proteins were kindly provided by colleagues: the MBP fusion protein ofthe NBD of the lactococcal half-transporter ... from the thermoacido-philic Sulfolobus solfataricus, by S. Albers (GroningenUniversity, the Netherlands).ATPase activity The ATPase activity of NBDs was measured by a colori-metric assay [ 34] ....