... resultfrom the modifications inthe interactions between the catalytic glutamates andthe residues R97 and Y331,indicating that these residues play a roleinthe modulation of the Sfbgly50 pH optimum.Sequence ... mMBicine buffer 7.0–8.5). The pH stability of Sfbgly50 was checked by incubation in the same buffers for a time equal to the assay time and thendetermining the activity remaining at the optimum ... [10,12].Nevertheless, it is not possible to assume that the same isoccurring inthe mutant R97M, because there is no data on the interactions between the substrate andthe arginineresidue.Finally, another...
... by the amount of internalized agonist[20]. As the internalization is expressed in percentage of total binding, decreasing the binding affinity of the Roleof helix 8 and C-termini in bradykinin ... 2004 FEBS The roleof helix 8 andofthe cytosolic C-termini in the internalization and signal transduction of B1 and B2bradykinin receptorsAlexander Faussner1, Alexandra Bauer1, Irina Kalatskaya1, ... addition of scintillation fluid. Nonspecific bindingwas determined inthe presence of 5 lm unlabeled agonist and subtracted from the total binding to calculate the spe-cific binding.Internalization of...
... of GSK3 (using Li or SB-415286)appears to be sufficient for inducing activation of GS in muscle and fat cells, and that inhibition ofthe kinasepotentiates insulin action in muscle of insulin-resistant ... increases in glucose uptake and glycogen synthesisallowing for more effective ÔchannellingÕ of glucose intoglycogen in response to insulin. However, one of the potential difficulties in interpreting ... phosphatidylinositol3-kinase, p70, S6 kinase and glycogen synthase kinase-3 activity in L6 muscle cells: evidence for the involvement ofthe mammaliantarget of rapamycin (mTOR) pathway in the L-leucine-inducedup-regulation...
... phenylalanine was thereforemutated to Ala, Leu, Trp or Tyr. These mutations may in uence the shape and volume ofthe acyl binding pocket and thereby alter the binding mode and affinity of the enzyme ... PAA and derivatives thereof, while maintaining the hydrophobicity ofthe binding site.To test the effect ofthe mutations on the specificity forphenylacetylated substrates, the steady-state kinetic ... kcatrepresents the rate of acylation and Kmequals the binding constant of the substrate to the free enzyme. The results then indicateÓ FEBS 2002 Roleof active-site phenylalanines in penicillin acylase...
... unsure ofthe mechanism of DNA-induced stabilization ofthe protein, and whe-ther it is related to the normal RNA-binding function of coat protein, as RNA binding had no effect. The increased ... understood, and we have investigated thesequestions in this work. We sought to determine the roleof protein–RNA and protein–protein interactions in virus stability, measuring the effects of urea,GdnHCl ... against all the forms of denatura-tion we tested. This is consistent with the observation of increased stability ofthe single-chain version of the wild-type dimer [30] and is reminiscent of the...
... visualized the mineralizingcells with 2% Alizarin Red. After rinsing in distilledwater and air drying the samples, we mounted the cov-erslips on microscope slides using Fluoromount and examined and ... result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing gene expression, cell growth and BMP-2 in gene expression and mineralization of bone.Materials and ... BMP-2, the yin and yang of mineralization:Contrast of effect of 24 hours of treatment with FGF-2 or BMP-2 on fold increase in abundance of mineralization-related geneexpression. Mineralizing...
... Li4AbstractIntroduction: The difficulty in re-growing and mineralizing new bone after severe fracture can result in loss of ambulation or limb. Here we describe the sequential roles of FGF-2 in inducing ... visualized the mineralizingcells with 2% Alizarin Red. After rinsing in distilledwater and air drying the samples, we mounted the cov-erslips on microscope slides using Fluoromount and examined and ... BMP-2, the yin and yang of mineralization:Contrast of effect of 24 hours of treatment with FGF-2 or BMP-2 on fold increase in abundance of mineralization-related geneexpression. Mineralizing...
... rationale for the use of taxol in DC immunotherapy and insights into the development of combinational DC therapies with other anticancer drugs that do not harm the viability or activity of DCs.Acknowledgments ... cells, producing major cytokines involved in immune responses. Importantly, taxol increased the expression ofthe anti-apoptotic protein Bcl-xL, and increased the mobilization of p65 subunit ... jooh@jejunu.ac.kr The roleof Bcl-xL and nuclear factor-κB inthe effect of taxol on the viability of dendritic cellsMi-Hyoung Kim1, Hong-Gu Joo1,2,*1Laboratory of Veterinary Pharmacology, College of...