... ofthe AUFB reactor Reactor Setup and Operation for theFormationof Aerobic Granular Sludge Two AUFB reactors were used for theformationof aerobic granular sludge Air was introduced from the ... reactors govern the washout of sludge from the reactor, and therefore, we investigated the effect of surface loading rate and aeration rate on the selection of well-settling sludge and theformation ... formed in our previous studies Based on the preliminary experimental results, we decided the control strategy for the selection of well-settling sludge Then, we applied the strategy to the formation...
... demonstrated, the incorporation of all the other subunits into the bc1 core structure proceeds normally, thus leading to theformationofthe bc1-oxidase supercomplexes [12] On the other hand, Qcr9p, ... which these putative sub-complexes bind to each other during the assembly ofthe bc1 complex is also unknown Furthermore, as in the case ofthe biogenesis of other multi-subunit complexes ofthe ... subunits incorporated into the bc1 complex On the other hand, the absence of Qcr9p in the DQCR9 ⁄ DQCR10 strain prevented the binding of ISP (Fig 4B) Interestingly, in the absence of Qcr9p, the catalytic...
... from the 695 nm band; therefore, the observed rate can be attributed to formationofthe Fe(III)–M80 axial bond, providing strong indication that the slow phase is coupled to formationofthe ... form, with further formationofthe LS (IHH) A-state; (b) for the slow phase, to formationofthe LS native-like (IHM) A-state Unlike wild-type cytochrome c, once the equilibrium is reached, the ... towards the bis-H species, and destabilize the M–Fe(III)–H coordinated form The reduced effect of Cl– reflects the different affinities ofthe two anions for the protein [11,17] We also monitored the...
... (ER), resulting in the loss of functional TNSALP from the cell surface [10–14] Deletion of T at position 1559 ofthe cDNA [TNSALP (1559delT)] ofthe TNSALP gene was reported for the first time in ... nomenclature, the adenine ofthe FEBS Journal 272 (2005) 1704–1717 ª 2005 FEBS initiator ATG codon in the cDNA of TNSALP is denoted as nucleotide +1 instead ofthe first nucleotide ofthe cDNA clone originally ... overexpression ofthe enzyme in the transiently transfected cells It is noteworthy that a 66 kDa form, but not an 80 kDa form was partitioned into the aqueous phase As the 66 kDa form ofthe wild type...
... that the data fit well with the individual determination of flux control coefficients by PFK, PK and LDH The role ofthe las operon is discussed on the basis ofthe distribution of flux control for ... mixed acid fermentation The data points for product formation were then fitted against the PK activities in order to determine the control exerted by PK over the flux to formate Because only one ... for PK activity below the wild-type level three or four curves were fitted for each ofthe studied metabolic fluxes using the best possible suggestions obtained using the software curveexpert The...
... to the emperor the domain ofthe Two Powers, 110 And the subordination ofthe temporal ruler in spiritual things, 111 The words of Gelasius have become the law ofthe Church, 113 The emperor ... 164 The bishop, clergy, and emperor accept the terms ofthe Pope, 165 The formulary of union signed by them, 167 The report ofthe legates to the Pope, 169 The emperor Justin's letter to the ... OFTHE NATIONS FROM ST LEO I TO ST GREGORY I by THOMAS W ALLIES, K.C.S.G Author ofthe "Formation Of Christendom"; "Church and State As Seen in theFormationof Christendom"; "The Throne of the...
... were constructed by use ofthe EcoRV and SacI sites in the cDNA and were then used to transform 293-cells The region responsible for the different degree of association ofthe human and murine enzyme ... catalytic domain of TPP II We can now report that the region surrounding Arg252 is of importance for theformationofthe oligomeric enzyme complex, which is a prerequisite for obtaining a fully ... about one tenth ofthe specific activity ofthe oligomeric complex [15] For the recombinant human enzyme the nonassociated form also appeared to be dimers ofthe 138 kDa subunit, since their Mr was...
... scales ofthe probability offormationof backbone–backbone hydrogen bonds (assigned to donor and to acceptor residues, termed donor and acceptor scales, respectively) The authors, in order to ... the scoring function as a physical property term Sphysprop consisting ofthe sum ofthe products ofthe amino acid frequency with the normalized property value ofthe respective amino acid for ... total number of hydrogen bonds this residue forms, acting either as donor or as acceptor, respectively, divided by the total number of residues ofthe same type in the database The two, apparently,...
... In an effort to investigate the effects of PFD on fibrillation of Ab(1–42) peptide, the major factor responsible for AD [1], Ab fibrillation was examined by monitoring levels ofthe fluorescent ... nm Fig Morphology of Ab aggregates formed in the presence of PFD (A) Ab fibrils formed in the absence of PFD Scale bar = 100 nm (B) Ab particles and protofibrils formed in the presence of PFD Arrows ... Fig Dot-blot assay of soluble Ab oligomers formed in the presence of PFD Samples of Ab oligomers formed in the presence of PFD (Ab ⁄ PFD), Ab fibrils formed in the absence of PFD (Ab fibril), A11-positive...
... distinguish the absolute configuration ofthe starting material, the ee ofthe compounds in the two layers will increase After the above mentioned separation procedure, the ee ofthe materials in the organic ... analysis for ketoprofenyl-CoA formation by LUC-H The substrate was incubated at 30 °C for 60 with LUC-H The ee ofthe recovered acid was determined by chiral phase HPLC analysis; the ee ofthe thioester ... way of an acyladenylate intermediate Kinetic analysis of LUC-H catalyzed thioester formationThe kinetic study oftheformationof ketoprofenylCoA catalyzed by LUC-H was examined to obtain the...
... In order to determine whether the transformation of preg into androstadienol requires prior synthesis of 17a-OHpreg or DHEA, we performed enzymatic assays using human and porcine P450c17 in the ... require prior formationof 17a-OH-preg and DHEA The lack of androstadienol synthesis in the presence of lM of ketoconazole (C), an inhibitor of cytochrome Fig Thin layer chromatography showing the transformation ... show the production of any intermediates in theformationof androstadienol which suggests that it is synthesized from pregnenolone in a single step Therefore, although the synthesis of glucocorticoid/sex...
... inhibitor covalent bound at the N5 position Fig Close-up ofthe crystal structure of MAO B The isoalloxazine ring of FAD is in yellow The axis ofthe pink helix points directly at the C2-O ofthe ... flavinylation mechanism The proposed mechanisms for covalent flavinylation via the C8a or C6 position ofthe isoalloxazine ring system could also be valid for theformationofthe bicovalent flavin–protein ... region ofthe protein In the cases presented in Table 2, there are potential bases near the residues that ˚ tether the flavin (4.2–5.6 A) Following deprotonation of C8a, or in the case of a thiolate...
... Formationofthe Union The second volume ofthe EPOCHS OF AMERICAN HISTORY aims to follow out the principles laid down for "THE COLONIES," the study of causes rather than of events, the development ... confidence was the rallying point ofthe military forces ofthe nation [Sidenote: Plans of campaign.] The theatre ofthe war was more favorable to the British than to the Americans There were no fortresses, ... ofthe colonies were leaders; but if they accepted too many ofthe governor's favors or voted for too many of that officer's measures, they found themselves left out ofthe assemblies by their...
... stabilizes the E1 conformation, leading to the accumulation ofthe species E1P in the phosphorylation experiment with ATP This conclusion is supported by the dephosphorylation studies in which the mutant ... possible that the tolerance for the substitutions at this site is related to the size and hydrophobicity ofthe substituting side chain Inspection of a theoretical model ofthe Ca-ATPase in the E2 state ... 50 lg of membrane protein was used The error bar is the standard deviation of three to four measurements In addition to measuring theformationof aspartyl phosphate, the catalytic cycle of a...
... books and on the internet to select the valuable information relating to the theme the forming ofthe plural nouns in English and Vietnamese equivalents Therefore, the content ofthe study is ... 2.1 Theformationof plural nouns in English 2.1.1 The simple nouns Theformationof plural nouns of variable nouns have two form: regular plural and irregular plural 2.1.1.1 .Regular plural forms ... definite article the before nouns form plural by adding -s suffix in nouns Singular form Plural form The Smith The Smiths (the Smith family) The Taylor The Taylors (the Taylor family) Eg: The Smiths...
... different from the Scorpaenifor- mes Both the orders belong to the same series, the Percomorpha The amino acid sequences of HCEs deduced from the newly cloned cDNAs are shown in Fig 6A All of them possessed ... suggest that the chorion-swelling activity in the stage 31 ovarian fluid is responsible for the action of HCEs, the genes of which are orthologous to those of other euteleostean HCEs Cloning of black ... digest the chorion In this study, we observed the embryo hatching ofthe ovoviviparous black rockfish Sebastes schlegelii, which is a member ofthe Scorpaeniformes within the Euteleostei [15] The...
... that of Ca2+-CaM [10,11,25,26], and therefore adversely affects binding of CaM to the Ca2+-CaM-binding domain of nNOS CaM is not only essential for the operation of nNOS and the other NOS isoforms, ... eukaryotic cells CaM is not only essential for the operation of nNOS and the other NOS isoforms, but is also the regulatory protein for a variety of kinase phosphorylating enzymes and adenylate cyclase ... hydrophobic anchors Given the length ofthe first helix of cupiennin 1a, and the number of hydrophobic anchors available, it seems likely that the cupiennin 1a ⁄ Ca2+-CaM complex is analogous to the structure...
... glycosyl residues linked to the N-terminal serines form the next ordered layer ofthe cable structure The surface layer ofthe cable is formed by a shell consisting of ordered water molecules NMR ... and the ST mutant of UK3 PVX elucidate the role ofthe PVX CP N-terminal region in determining the physicochemical properties ofthe virion surface, preservation ofthe virion integrity, and the ... that the presence of an ordered water column around PVX virions, by itself and /or through formationof a water-mediated net of hydrogen bonds between (or inside) CP subunits, strongly affects the...
... in the MALDI-TOF spectrum of tryptic fragments ofthe wildtype cytochromes The appearance of some ofthe Lys70containing peptide of residues #64–78 (lacking heme) in each ofthe spectra ofthe ... would be formed That the chromophore was not formed may result from differences in the chemical nature ofthe lysyl- or tyrosyl- linkage orthe relative orientation of K70 and Y70 in the cytochromes ... confirming the role ofthe crosslink in determining the Soret spectrum Although catalytic activity was lost in the mutants, the ligand-binding capability and thus the pentacoordinate nature ofthe heme...