... possible role of this chaperone in the stabilization ofthe core struc-ture ofthe bc1complex can be excluded on the basis of the existence ofthe 500 kDa bc1sub-complex alsoin the DBCS1 deletion ... to the 500 kDa bc1sub-complex. Therefore, the pres-ence of both Qcr9p and Bcs1p is required for the inser-tion of ISP into the bc1sub-complex, but the presence of only one of these two ... alsounknown. Furthermore, as in the case ofthe biogene-sis of other multi-subunit complexes ofthe mitochon-drial respiratory chain, the assistance of specificchaperone proteins is also required. The...
... analysis ofthe las operonFEBS Journal 272 (2005) 2292–2303 ª 2005 FEBS 2293minute. The relative values of simultaneous modulation of the three las enzymes are calculated as the average of the three ... were related to the A280 of the extract, for the purpose of determining relative activities. The specific activ-ities of PFK and PK and LDH in MG1363 were deter-mined as UÆmg)1 of protein, where ... each ofthe las enzymes on the glycolytic flux,growth rate and product formation. We also studiedstrains with modulated expression ofthe entire lasoperon, and show that the data fit well with the...
... the beginning of his dignity to the See ofthe Apostle Peter. For the newly-elected pontiff acknowledges in hisletter that Peter is the chief ofthe Apostles and the Rock ofthe Faith: that the keys ofthe ... the beginning ofthe fifth century was justified by the experience of Theodorich at the beginning ofthe sixth. And, further, we may take the conduct of these two great men asexpressing on the ... 455 is the first of three hundred years of warfare carried on from the time ofthe Vandal through the time of the Lombard, under the neglect and oppression ofthe Byzantine, until, in the year...
... over-expressing the Arg252-variant, thesedimers may contribute to theformationof active oligomersin the p resence ofthe endogenously expressed G ly252-containing subunits. T he exact c omposition ofthe ... been ascribed to the insert between Asp and His ofthe catalytic domain of TPP II. We can now report that the region surroundingArg252 is of importance for theformationofthe oligomericenzyme ... bout one tenth of the specific activity ofthe oligomeric complex [15]. For the recombinant human enzyme the nonassociated form alsoappeared to be dimers ofthe 138 kDa subunit, since theirMrwas...
... of a4v over the entire sequence defines the aggregationprofile ofthe polypeptide. The hot-spot threshold(HST) was defined as the average ofthe a3v ofthe 20natural amino acids weighted by their ... assigned to the central residue ofthe window. The smoothed expectedvalues for every position ofthe polypeptide chain pro-vide the final profile, which is directly used for the pre-diction of amyloidogenic ... propensity enter the scoring functionas a physical property term Sphyspropconsisting of the sum ofthe products ofthe amino acid frequency with the normalized property value ofthe respective...
... require prior formationof 17a-OH-preg andDHEA. The lack of androstadienol synthesis in the presence of 1 lM of ketoconazole (C), an inhibitor of cytochromeP450, further demonstrates the specific ... tissues such as the liver and adipose tissue. Ourstudy aimed at elucidating the nature and the mechanism of the reactions involved in the local formationof androstenolis thus of major importance.AcknowledgementsThis ... catalyzes the transformation of preginto androstadienol, a precursor in the biosynthesis of Fig. 5. Influence of increasing concentrations of cyt b5on the relative formation of androstadienol and...
... than the wild-type both in the absence a nd in the p resence of Fig. 3. Normalized zinc-dependent ATPase activity ofthe wild-type andmutated enzymes. TheATPaseactivity(percentageoftheactivityofthewild-type) ... arked reduction in the formationofthe ADP-sensitive phosphointermediate. The mutants ofthe HP motif (H475Q and P476L) showATP-driven phosphorylation l evels of 32% and 26% of the wild-type, respectively. ... The effect of 30 lMZn2+on the Pi-phosphorylation ofthe wild-type and the P401Lenzymes (gray columns). Note that the maximal phosphorylation of the P401L mutant is only 8% ofthe wild-type...
... Table of contents Acklowledgements Part one: introduction 1. Rationale ofthe study 1 2. Aims ofthe study 2 3. Method ofthe study 2 4. Scope ofthe study 2 5. Design ofthe study ... preposition, the complement , the prepositional object, and the object of preposition. The subject of a verb Eg: A plane is taking off (Khuª,1999: 27) The direct object of a verb Eg: ... point out the rationale, aims, methods, scope and design of the study. The second is development which consists of three chapters: - The chapter one is theoretical background is given for the study....
... domain of nNOS. CaM is notonly essential for the operation of nNOS and the otherNOS isoforms, but is also the regulatory protein for avariety of other enzymes, including kinases [27–29].Two ... Ca2+-CaM when itbinds to the CaM-binding domain of endothelial nitricoxide synthase (eNOS) [33]. The second example iswhen the C-terminal lobe of Ca2+-CaM binds part of the target peptide. This ... Fig. 4B for the 20-residue binding domain ofthe plasma membrane Ca2+pump ⁄ Ca2+-CaM complex, where the first 12 residues of the peptide are encompassed by the C-terminal end of Ca2+-CaM...
... less sensitive to the pres-ence ofthe anion. These residues also modulate the conformation of unfol-ded cytochrome c, influencing its spin state and the coordination to the prosthetic group.AbbreviationCT, ... with further formationofthe LS(IHH) A-state; (b) for the slow phase, to formationof the LS native-like (IHM) A-state. Unlike wild-typecytochrome c, once the equilibrium is reached, the K88E ... from the 695 nm band; therefore, the observed rate can be attributed to formationof the Fe(III)–M80 axial bond, providing strong indicationthat the slow phase is coupled to formationof the native...