... less sensitive to the pres-
ence ofthe anion. These residues also modulate the conformation of unfol-
ded cytochrome c, influencing its spin state and the coordination to the
prosthetic group.
Abbreviation
CT, ... with further formationofthe LS
(I
HH
) A-state; (b) for the slow phase, to formation of
the LS native-like (I
HM
) A-state. Unlike wild-type
cytochrome c, once the equilibrium is reached, the
K88E ... from the 695 nm band; therefore, the
observed rate can be attributed to formationof the
Fe(III)–M80 axial bond, providing strong indication
that the slow phase is coupled to formationof the
native...
... analysis ofthe las operon
FEBS Journal 272 (2005) 2292–2303 ª 2005 FEBS 2293
minute. The relative values of simultaneous modulation of
the three las enzymes are calculated as the average of the
three ... were related to the A
280
of the extract, for the
purpose of determining relative activities. The specific activ-
ities of PFK and PK and LDH in MG1363 were deter-
mined as UÆmg
)1
of protein, where ... each ofthe las enzymes on the glycolytic flux,
growth rate and product formation. We also studied
strains with modulated expression ofthe entire las
operon, and show that the data fit well with the...
... over-expressing the Arg252-variant, these
dimers may contribute to theformationof active oligomers
in the p resence ofthe endogenously expressed G ly252-
containing subunits. T he exact c omposition ofthe ... been ascribed to
the insert between Asp and His ofthe catalytic domain of
TPP II. We can now report that the region surrounding
Arg252 is of importance for theformationofthe oligomeric
enzyme ... bout one tenth of
the specific activity ofthe oligomeric complex [15]. For the
recombinant human enzyme the nonassociated form also
appeared to be dimers ofthe 138 kDa subunit, since their
M
r
was...
... outside the landward fortifications
of the city; the fate of Canada was decided in a battle in the open; the dying Wolfe defeated the dying
Montcalm, and the town surrendered. The fall ofthe rest of ... Down to the day ofthe Declaration of Independence the advantage was
clearly with the colonists. The hard, stern struggle ofthe war began in August, 1776, with the arrival of the
British in the ... 38
The second volume ofthe EPOCHS OF AMERICAN HISTORY aims to follow out the principles laid down
for " ;THE COLONIES," the study of causes rather than of events, the development of the...
... possible role
of this chaperone in the stabilization ofthe core struc-
ture ofthe bc
1
complex can be excluded on the basis
of the existence ofthe 500 kDa bc
1
sub-complex also
in the DBCS1 deletion ... to the 500 kDa bc
1
sub-complex. Therefore, the pres-
ence of both Qcr9p and Bcs1p is required for the inser-
tion of ISP into the bc
1
sub-complex, but the presence
of only one of these two ... also
unknown. Furthermore, as in the case ofthe biogene-
sis of other multi-subunit complexes ofthe mitochon-
drial respiratory chain, the assistance of specific
chaperone proteins is also required. The...
... assembly of TNSALP in the
endoplasmic reticulum (ER), resulting in the loss of
functional TNSALP from the cell surface [10–14]. Dele-
tion of T at position 1559 ofthe cDNA [TNS-
ALP (1559delT)] ofthe ... [16–18]. According
to the recommended nomenclature, the adenine of the
initiator ATG codon in the cDNA of TNSALP is
denoted as nucleotide +1 instead ofthe first nucleotide
of the cDNA clone originally ... specific enzyme activity
of the mutant protein was calculated to be about one
third of that ofthe wild type (Fig. 7C). In contrast to
the culture medium ofthe cells expressing the wild
type enzyme,...
... the
beginning of his dignity to the See ofthe Apostle Peter. For the newly-elected pontiff acknowledges in his
letter that Peter is the chief ofthe Apostles and the Rock ofthe Faith: that the keys ofthe ... the beginning ofthe fifth century was justified by the experience of
Theodorich at the beginning ofthe sixth. And, further, we may take the conduct of these two great men as
expressing on the ... 455 is the
first of three hundred years of warfare carried on from the time ofthe Vandal through the time of the
Lombard, under the neglect and oppression ofthe Byzantine, until, in the year...
... of
a4v over the entire sequence defines the aggregation
profile ofthe polypeptide. The hot-spot threshold
(HST) was defined as the average ofthe a3v ofthe 20
natural amino acids weighted by their ... assigned to the
central residue ofthe window. The smoothed expected
values for every position ofthe polypeptide chain pro-
vide the final profile, which is directly used for the pre-
diction of amyloidogenic ... propensity enter the scoring function
as a physical property term S
physprop
consisting of the
sum ofthe products ofthe amino acid frequency with
the normalized property value ofthe respective...
... range of substrates through a
set of hydrophobic residues located at the tips of the
tentacles [34,35,39,52], it has also been shown that there
are basic residues in the distal regions ofthe tentacles ... support the notion that PFD
participates in theformationof Ab oligomers within
the cytosolic compartment.
In an effort to elucidate the mechanism pertaining
to the PFD-induced formationof high-molecular-mass
soluble ... oligomer
Inhibit/Slow down oligomerization
Aβ protofibril
Fig. 8. Schematic model oftheformation of
soluble Ab oligomers in the presence of
PFD.
Formation of amyloid beta oligomers by prefoldin M....
... absolute configuration of the
starting material, the ee ofthe compounds in the two
layers will increase.
After the above mentioned separation procedure, the
ee ofthe materials in the organic layer ... interesting from the
view point ofthe molecular recognition of this enzyme.
The 3D structural analysis may shed light on the
answers of these questions.
In the case of recombinant rat LACS1, the substrate
specificity ... acid.
Because the ee ofthe thioesters could not be deter-
mined as they existed, the thioester bond was hydro-
lyzed and the ee ofthe resulting free acid was measured.
The two kinds of hydrolytic...