methodology biology and clinical applications of human mesenchymal stem cells
Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche, ... stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991 ;Mesenchymal stem ... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal...
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Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal stem ... D-23 to AFS and AR from the Interdisciplinary Center for Clinical Research (IZKF) Würzburg References Kolf CM, Cho E, Tuan RS: Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation ... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs,...
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Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
... phosphorylation status of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater ... differentiation of size-sieved stem cells into electrically active neural cells Stem Cells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved stem cells ... differentiation of human mesenchymal stem cells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal stem cells...
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Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2 ... fracture repair Materials and methods All chemicals used were from Sigma-Aldrich (St Louis, MO, USA), unless stated otherwise Culture of human mesenchymal stem cells Human MSCs were obtained from...
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Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured with human MSCs in the Ost medium on day 28 Figures C and ... Chan, and Seeram Ramakrishna Mineralized electrospun nanofibrous scaffolds for directing osteogenic differentiation of human mesenchymal stem cells Proceedings of the 241st ACS Meeting and Exposition, ... Table 2.9 Classification of stem cells [23-30] 51 Table 2.10 Advantages and disadvantages of fabrication methods of nanofibers 79 Table 2.11 Clinical trials using nanofibers 88 Table...
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The derivation, propagation, storage and gene expression of human embryonic stem cells on human feeders
... somatic stem cells can be found in diverse tissues and organs The most common adult stem cells are the hematopoietic stem cells, mesechymal stem cells and neuronal stem cells Adult stem cells have ... of cells make up the human body viz., germ cells, somatic cells and stem cells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated ... classes of pluripotent stem cells in humans, other primates and mice These are embryonic stem cells, embryonic germ cells, embryonic carcinoma cells and recently the discovery of a fourth class of...
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RADIATION IN TISSUE BANKING Basic Science and Clinical Applications of Irradiated Tissue Allografts doc
... and Dose Validation Norimah Yusof 259 Chapter 20 Quality System in Radiation Sterilization of Tissue Grafts Nazly Hilmy 275 Part V CLINICAL APPLICATIONS OF IRRADIATED BONE GRAFTS Chapter 21 Clinical ... irradiate soft tissues? Until today, soft tissues have never been irradiated for fear that the dose of 25 kGy is too large and could weaken the collagen structure of tendons and ligaments These and ... grafts The clinical applications of irradiated bone grafts are described in Part V, and the applications of irradiated amnion grafts in Part VI This book includes three valuable sources of information...
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Báo cáo hóa học: "Future research and therapeutic applications of human stem cells: general, regulatory, and bioethical aspects" ppt
... functional repair of myocardial infarction, and in stroke [31,32] Other types of stem cells Hematopoietic stem cells together with mesenchymal stem cells, the so-called “side population”, and multipotent ... progenitor cells (MAPCs), are the stem cells Page of 15 forming bone marrow [33] Their role is maintenance and turnover of blood cells and immune system The high rate of regeneration of the liver, ... Induced pluripotent stem cells Induced pluripotent stem cells (iPSCs) from somatic cells are revolutionizing the field of stem cells Obtained by reprogramming somatic stem cells of a patient through...
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Báo cáo y học: "Biology of adult mesenchymal stem cells: regulation of niche, self-renewal and differentiation" potx
... mesenchymal stem cells: characterization, differentiation, and application in cell and gene therapy J Cell Mol Med 2004, 8:301-316 31 Tuan RS, Boland G, Tuli R: Adult mesenchymal stem cells and ... is critical for self-renewal of human multipotent adipose-derived stem cells Stem Cells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation of stem cell function Curr Opin ... the stem state of MSCs [14] and other stem cells [15] LIF also activates and represses osteoblast and osteoclast activities [20] The bipotency of LIF suggests that the cellular environment and...
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Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells ... J Chan Human endothelial progenitor stem cells enhances osteogenic response of bone marrow derived human fetal mesenchymal stem cells through paracrine signalling mechanisms in vitro and induces ... Zhang, MA Choolani, J Chan Human endothelial progenitor stem cells accelerates and potentiates the osteogenic response of bone marrow derived human fetal mesenchymal stem cells through paracrine...
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báo cáo hóa học:" MicroRNA and gene expression patterns in the differentiation of human embryonic stem cells" doc
... either EB or adult cells (Figure 6, panel A) The difference in the expression of miR-200c, miR-302b, and miR-367 between hES cells and EB, and between hES cells and adult cells was significant ... in adult cells (Figure 6, panel C) The differences in expression of these miRNA expression between adult cells and hES cells and between adult cells and EB were significant (P < 0.05) Of note, ... expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in two types of adult cells HMVEC and HUVEC Differences in the expression of EB signature...
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báo cáo khoa học: "Adenovirus-mediated siRNA targeting Bcl-xL inhibits proliferation, reduces invasion and enhances radiosensitivity of human colorectal cancer cells" ppt
... overexpression of Bcl-xL is associated with radioresistance of human osteosarcoma cells In other human cancers, the association of Bcl-xL expression and radiosensitivity of tumor cells is also ... **P
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báo cáo khoa học: "Epigenomics of human embryonic stem cells and induced pluripotent stem cells: insights into pluripotency and implications for disease" potx
... Figure The combination of stem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses of cells derived through in vitro stem cell differentiation ... embryonic stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone ... embryonic stem cells and differentiation Nat Rev Mol Cell Biol 2006, 7:540-546 51 Park SH, Kook MC, Kim EY, Park S, Lim JH: Ultrastructure of human embryonic stem cells and spontaneous and retinoic...
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Báo cáo y học: "Discrepancy between the in vitro and in vivo effects of murine mesenchymal stem cells on T-cell proliferation and collagen-induced arthritis" doc
... Page of 11 Figure Mesenchymal stem cells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... http://arthritis-research.com/content/12/1/R31 Page of 11 Figure Phenotype and differentiation potential of mesenchymal stem cells (MSCs) Bone marrow cells of DBA/1 wild-type and interferon-gamma receptor knockout ... of 11 Mesenchymal stem cell treatment has no effect on the development of collagen-induced arthritis Figure Mesenchymal stem cells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells...
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Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot
... cell-mediated mesenchymal stem cell differentiation Stem Cells 2006, 24:707-716 Le Visage C, Kim SW, Tateno K, Sieber AN, Kostuik JP, Leong KW: Interaction of human mesenchymal stem cells with disc cells: ... Multilineage potential of adult human mesenchymal stem cells Science 1999, 284:143-147 Wuertz K, Godburn K, Neidlinger-Wilke C, Urban J, Iatridis JC: Behavior of mesenchymal stem cells in the chemical ... top of the suspension and cells were visualized at 20 × Excitation and emission for Calcein and ETH-2 were 494/517 nm and 528/617 nm respectively with Calcein staining the cytoplasm of live cells...
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Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
... Sumitha, Aji and Tamanna, and Susan, thank you for the joy you bring to my life and my world Pappa and Mamma, thank you for your love and unconditional support My grandparents and the rest of my extended ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stem cells The isolation of mouse and human embryonic stem cells (ES cells) heralded a new era in regenerative medicine, raising hopes ... isolation and culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) of human embryonic stem...
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Transcriptome study of human embryonic stem cells and knockdown study of a pluripotency marker, LIN28
... Introduction 1.1 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells Embryonic stem (ES) cells are isolated from the inner cell mass (ICM) of embryos of blastocyst ... List of Figures VIII List of Abbreviations IX Introduction 1.1 1.2 Human embryonic stem cells 1.1.1 Overview and characteristics of human embryonic stem cells 1.1.2 Regulatory networks and transcription ... Materials and methods 22 2.1 Culture of human ES cell line 22 2.1.1 Preparation of feeder cells 22 2.1.2 Maintenance of human ES cells 22 2.1.3 Preparation of embryoid bodies 22 2.2 Culture of NCCIT...
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Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
... An efficient and safe xeno-free cryopreservation method for the storage of human embryonic stem cells Stem Cells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes ... cannot be said of human embryos, which are much less robust and hardy compared to mouse embryos Hence laboratories involved in handling human embryos in clinical assisted reproduction often have ... include quick handling outside the incubator, as well as the use of pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes...
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Atlas of Human Pluripotent Stem Cells doc
... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion of human embryonic stem cells, and more recently, of human induced pluripotent stem cells The ... long-term culture, expansion and manipulation of human pluripotent stem cells The culmination of two events has enabled the production of human embryonic stem cells: the birth of the first IVF test-tube ... the culture of undifferentiated hESCs, including human fetal-derived M Amit and J Itskovitz-Eldor, Atlas of Human Pluripotent Stem Cells: Derivation and Culturing, Stem Cell Biology and Regenerative...
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báo cáo hóa học:" Elevated adipogenesis of marrow mesenchymal stem cells during early steroid-associated osteonecrosis development" pptx
... marrow lipid was a consequence of the adipogenesis of marrow mesenchymal stem cells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... like to thank Professor Huang Lin and Miss Winnie lee from the Department of Orthopedics and Traumatology, the Chinese University of Hong Kong, for their assistance in cells culture and related evaluation...
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