... differentiate from mesenchymalstromalcells (MSCs) MSCs are multipotent cells that can replicate as undifferentiated cellsand that have the potential to differentiate to lineages ofmesenchymal tissues, ... levels of mCRY1 decreased after blue laser irradiation relative to non-irradiated cells (Fig 1C) Protein products of CRY act together with PER proteins to inhibit CRY and PER transcription and close ... center of plate well) were observed by fluorescence microscopy To evaluate osteogenesis, MSCs were rinsed three times with PBS and fixed with 4% formalin in PBS Fixed cells were incubated with...
... transcripts Expressionof BMP-2 and chordinstem cells during osteogenic differentiation ofhumanmesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... LV, Cheng SL: Regulation of bone matrix proteinexpressionand induction of differentiation ofhuman osteoblasts andhuman bone marrow stromalcells by bone morphogenetic protein- 2 J Cell Biochem ... using WST-1 assay at 3, and days of culture After and days, cells treated with chordin siRNA had decreased proliferation, as compared with untreated cells, andcells treated with transfection reagent...
... differentiation ofmesenchymal progenitor cellsand to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... differentiation of cultured humanmesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis ofhumanmesenchymal ... by red fluorescence, compared with the Ad.Luc transduced (Figure 5a) and untrans- Figure Immunohistochemical analyses for cartilage matrix proteins of MSC pellets after chondrogenic induction with...
... increases the expressionof insulin and Glut2, and transduction with both siERK and siAkt decreases the expressionof insulin and Glut2 (mean ± S.D.; *p < 0.05 and **p < 0.01 compared with the scrambled ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM ... between the expressionof these genes in cells treated with or without ECM We then quantified the gene expressionof insulin and Glut2 RT-PCR revealed adding FN or LAM increased the expressionof Insulin...
... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured withhuman MSCs in the Ost medium on day 28 Figures C and ... OPN expression between PLLA and TCPS In Figure b, OCN expressionofhuman MSCs on the PLLA nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of ... gene expressionofhuman MSCs cultured on PLLA and Dexloaded PLLA scaffolds in the growth and Ost-Dex media after 14 and 21 days: (A) ALP, (B) OCN, (C) BSP, and (D) Cbfa1 Significant difference of...
... 1991 ;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromalcells Biology of adult mesenchymal stem cells: regulation of ... differentiation ofhumanmesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation ofhumanmesenchymal ... 1988;Marrow stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stem cellsand the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991;Mesenchymal...
... column (Amersham Biosciences) (100 mg protein for mL of gel), washed with 50 mL of buer A and 10 mL of buer A containing 0.3 M NaCl, before elution of the proteinwith buer A containing M NaCl Further ... ovalbumin and chymotrypsinogen as markers The elution volume of UMP-CMP kinase indicated a Stokes radius of 2.8 nm, in agreement with a monomer species Expressionand purication ofhuman UMP-CMP ... groups with catalytic efciencies in the range of 107 M)1ặs)1 for UMP and CMP, andof 105 M)1ặs)1 for AraCMP, L-3TCMP and dCMP (Table 1) Careful characterization of the reaction catalysed by human...
... incubated with allogeneic T -cells stained with CFSE After days of co-culture, percentages of CD3+ dividing T cells measured by flow cytometry were linearly correlated with the loss of CFSE fluorescence ... function of natural killer cells, B cells, T cellsand myeloid DC [16] Human pDC not express lineage specific markers, but are characterized by the expressionof HLA-DR, CD4, CD123, BDCA2 and BDCA4 ... and non-adherent cells in culture were harvested at day and transduced by LV-VSVG-PGK at an MOI of 18 TU/ml and at a fixed concentration of × 106 cells/ ml, in RPMI 1640 After hours at 37°C, cells...
... and Fraser 2005) However, unhindered analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of ... lining of the embryo consists of both VE and DE cells (not exclusively VE) (3) Absence ofexpressionof VE-specific genes is due to down regulation and not cell migration (4) VE-derived cells ... Pancreas and diabetes The pancreas consists of Acinar cells which perform its exocrine functions and clusters ofcells called Islets of Langerhans which perform its endocrine functions Acinar cells...
... of growth factors on proliferation ofhuman NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation ofhuman NP cells (1%FBS/F12) (n = 20) Cells were incubated with ... significant increase compared to the control group after 3, 5, and days of culture Compared with the data of standard medium at the days, the proliferative percence of TGFβ1, IGF-1 and TGF-β1+IGF-1 on ... significant stimulating effect of TGF-β1 over doses of 1.0–500.0 μg/L on NP cells, and the maximum stimulus with 10 μg/L of the TGF-β1 reached a 72.4% increase compared with the standard medium The results...
... epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitor cells ... 105, and × 104 cells per well, in triplicate) were cultured in expansion medium for weeks, fixed with 10% formalin, and stained with Giemsa (SigmaAldrich), and colonies of fibroblast-like cells ... supplements, with controls including T-MPCs and BM-MPCs of the same passage maintained and cultured in expansion medium, and BM-MPCs cultured in adipogenic medium Morphological changes in BM-MPCs and...
... up-regulated during immortalizationof PBMCs with HTLV-1 To determine the temporal expressionof PTHrP during HTLV-1 immortalizationof PBMCs, PTHrP mRNA (Figure 2A) andprotein (Figure 2B) expression ... factor in conjunction with other oncogenes The goal of this study was to investigate the expressionof PTHrP, its receptor, and MIP-1α during the early stages ofimmortalizationofhuman lymphocytes ... during immortalizationof PBMCs with HTLV-1 infection PTHrP was markedly up-regulated during immortalizationof PBMCs with HTLV-1 infection (A) PTHrP mRNA expression during immortalizationof PBMCs...
... characterization ofhuman TReg cells by studying 350 TReg cell associated genes selected on the basis of whole-genome transcription data from humanand mouse TReg cells Application of our nonredundant Human ... restimulation via their TCR (Figure 2) Coexpression of GATA3 and FOXP3, but the lack of T-bet, suggests similarities in the gene expression profiles of Th2 and TReg cells in humans refereed research This ... regulatory phenotype and the amount of Foxp3+ cells within MACS purified human CD4+CD25+ T cells (Additional data file 5); and a Word table describing the phenotype of contaminating cells within MACS...
... my two supervisors Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they ... Teoh Coculture ofhuman endothelial progenitor stem cellsand bone marrow-derived human fetal mesenchymal stem cells potentiates osteogenesis through paracrine activity in vitro and induces neovasculogenesis ... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells...
... colony with distinct sharp boundaries together with strong expressionof both SSEA-3 and TRA-1-81; (2) Grade B that was partially differentiated, with some areas of nonuniform cell morphology and ... Oberkochen, Germany) with an HBO-103 mercury lamp and filter sets for FITC, Cy3.5, Texas Red, Cy5, Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems ... carried out with telomere-specific PNA probe (5 μg/ml) labeled with Cy3 (red fluorescence under an excitation wavelength of 559 nm), and centromere-specific PNA probe (30 μg/ml ) labeled with FITC...
... Number ofcells × 105 iPLA2-VIA by treatment ofcellswith bromoenol lactone (BEL) [11,12] In INS-1 insulinoma cells, acute inhibition of iPLA2-VIA reduces the relatively high content of lysoPC of ... shows that treatment of the cellswith BEL induced a significant accumulation ofcells in G2 ⁄ M and S, and a concomitant decrease ofcells in G1, with respect to untreated cells In contrast, pyrrophenone ... as the cells entered G1 and then increasing again as the cells approached and entered S phase The same pattern of variation of iPLA2 activity was detected whether the assay was conducted with mixed...
... subsets are expanded by interaction with SSA Some authors indicated clonal expansion of T -cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation ofhuman T -cells bearing ... linear NaCl gradient About 15 mg of purified protein per litre of culture medium was obtained Reduction and alkylation of SSA SSA after Ni–NTA purification was reduced with 10 mM DTT for h at 25 °C ... immunization with mgÆmL)1 of SSAia mixed with a volume of complete Freund’s adjuvant Boosts were administered on day 7, 14 and 28 Sera obtained on day 35 were diluted 10-fold and tested by ELISA and immunoblot...
... instrument Reduction, carboxamidomethylation and tryptic digestion of HCII from plasma and from recombinant CHO cells One to two nanomoles of the purified protein were reduced, carboxamidomethylated and ... properties of HCII Here, we report on the structure of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cellsand their effects on heparin binding MATERIALS AND METHODS ... were filled with lL of this solution The tip of the capillary was placed orthogonally in front of the entrance hole of a QTOF II mass spectrometer (Micromass, Manchester, England) equipped with a...
... lysates, immunoprecipitations, and preparation of cytoplasmic and nuclear fractions Human L540 and HEK293 cellsand monkey COS-7 cells were cultivated under standard conditions as described FEBS ... particularity of the SMN protein is its demand for the presence of Arg ⁄ Gly-rich regions in most of its inter- Fig Localization of Ki-1 ⁄ 57 to nucleoli and splicing speckles HEK293 cells were ... colocalization of Ki-1 ⁄ 57 with SMN ⁄ GEMS in Adox-treated cells (Fig 7Biii) Despite the diffuse nuclear distribution of EGFP–Ki-1 ⁄ 57, the majority of the red GEMS spots coincide with spots of brighter...