... synthesizes large quantities of cholesterol and phospholipids, which are either packaged with lipoproteins and transport to the rest of the body, or excreted in bile as cholesterol or bile acids after ... arthrogryposis-renal dysfunction-cholestasis (ARC) syndrome, which is the result of a mutation in another class C vps gene The tumor suppressor gene nf2 mutant develops extrahepatic choledochal cysts in ... to CLSD crusher mutants appear normal until the onset of craniofacial chondrogenesis Mechanism study revealed that crusher chondrocytes accumulate proteins in a bloated ER, resulting in severe...
... overnight Embryos younger than 15 hpf were fixed before dechorionation and the chorion was removed afterwards Embryos older than 16 hpf were dechorionated before fixation After fixation, the embryos ... organizing centre of the ventral neural tube is the axial mesoderm, which is composed of the notochord and prechordal plate mesoderm The second organizing 25 centre is the floor plate, and is established ... notochord, showing the presence of zfgfap-l positive cells in the posterior neural tube dc-diencephalon; e-eye; fb-forebrain; mb-midbrain; hb-hindbrain; mhb-midbrainhindbrain boundary; n- notochord;...
... cells (gc), inner plexiform layer (ipl), inner nuclear layer (inl), outer nuclear layer (onl), choroid (ch) and sclera (sc) Dark staining in the pigmented epithelium (pe) was also observable ... described for yeast [42] On the other hand, STK33 orthologs are present in the genomes of several chordate organisms (Fig 7), but not found in yeast, fly or nematode genomes This is not too surprising ... et al STK33 expression pattern Fig Amino acid sequence alignment of Stk33 kinase domain in some chordates Proteins sequences from human (Hsa) and mouse (Mmu) as described in [1] Sequences from...
... effects with the BCL11B gene by decreasing mitochondrial cytochrome c release inhibitory action of the Wnt target gene osteopontin (SPP1) on mitochondrial cytochrome c release determines renal ... of T-ALL Recent evidence has suggested that multiple mechanisms may regulate the release of mitochondrial factors, some of which depend on the action of caspases BCL2L1 may inactivate caspase-8 ... formation in the plasma membrane, nucleus, and Golgi complex while diverting DISC formation to the mitochondria The inhibitory effects of BCL2L1 on DISC formation may play a significant role in protecting...
... Tris/HCl pH 8.0, 150 mM NaCl, 0.5% deoxycholate, 0.1% SDS, 1% NP-40, mM EDTA), once with high salt buffer (50 mM Tris HCl pH 8.0, 500 mM NaCl, 0.1% SDS, 0.5% deoxycholate, 1% NP-40, mM EDTA) once with ... Altered processing of precursor transcripts and increased levels of the subunit I of mitochondrial cytochorme c oxidase in syrian hamster fetal cells initiated with ionizing radiation Carcinogenesis ... cDNA synthesis was performed with lL of DNase-treated total RNA (0.1 lgÆlL)1), lL of oligo(dT) anchored primer (2 lM) 12 and lL dNTP mix (250 lM) (1 : : : 1) (v/v/v/v) using SuperScriptTM RNase...
... of SN compared to ventral tegmental area [40] Mitochondrial dysfunction is another event implicated in PD pathogenesis and inhibition of mitochondrial complex I and III leads to elevated levels ... RalDH-I and the mouse homolog was formerly known as AHD2 There are two other classes of ALDHs, mitochondrial ALDH2 and stomach cytosolic ALDH3, neither of which has been found to participate in RA ... Neurobiol Dis 14, 637–647 Grimm J, Mueller A, Hefti F & Rosenthal A (2004) Molecular basis for catecholaminergic neuron diversity Proc Natl Acad Sci USA 101, 13891–13896 Mandel S, Grunblatt E, Riederer...
... Placenta Chorioncarcinoma Primary tumor Produce hCG, HCS, progesterone [36,37] JAR Placenta Chorioncarcinoma Primary tumor Produce estrogen, progesterone, hCG, HCS [38] BeWo Placenta Chorioncarcinoma ... for RT-PCR analysis: endometrium carcinoma (Endo-CA), cervical carcinoma (Cervix-CA), chorion carcinoma (Chorio-CA), ovarian carcinoma (OV-CA) and teratocarcinoma (Terato-CA) Primers, specific ... in comparison to the abovementioned cell lines, BeWo cells could be the best choice for future experiments concerning chorioncarcinoma cell lines and the Ishikawa cell line concerning endometrial...
... of myelin-like structures in Cyprinus carpio hepatocytes, swelling of mitochondria and disappearance of the internal mitochondrial membrane in carp at both exposure concentrations [17] However, ... pesticide dimethoate on cardiac and acetylcholinesterase activity in the shore crab, Carcinus maenas Aquat Toxicol 40, 23–26 69 Fulton MH & Key PB (2001) Acetylcholinesterase inhibition in estuarine ... Bocquene G, Roig A & Fournier D (1997) Cholinesterases from the common oyster (Crassostrea gigas) FEBS Lett 14, 1–6 ´ 71 Bocquene G & Galgani F (1998) Cholinesterase inhibition by organophosphorous...
... has yet to be defined; the concomitant administration of cisplatin represents a widely accepted choice It has been reported that induction chemotherapy (IC) with cisplatin and fluorouracil (PF) ... staining intensity to obtain a final semi-quantitative H score The median value of the H score was chosen as the cutoff point for separating low and high levels of ERCC1 expression [22] survival ... JL, De Raucourt D, Rhein B, Wibault P, Marandas P, Coche-Dequeant B, Stromboni-Luboinski M, Sancho-Garnier H, Luboinski B: Randomized trial of neoadjuvant chemotherapy in oropharyngeal carcinoma...
... has yet to be defined; the concomitant administration of cisplatin represents a widely accepted choice It has been reported that induction chemotherapy (IC) with cisplatin and fluorouracil (PF) ... staining intensity to obtain a final semi-quantitative H score The median value of the H score was chosen as the cutoff point for separating low and high levels of ERCC1 expression [22] survival ... JL, De Raucourt D, Rhein B, Wibault P, Marandas P, Coche-Dequeant B, Stromboni-Luboinski M, Sancho-Garnier H, Luboinski B: Randomized trial of neoadjuvant chemotherapy in oropharyngeal carcinoma...
... Sedgwick RP: Ataxia-telangiectasia (Clinical and immunological aspects) Psychiatr Neurol Med Psychol Beih 1970, 13-14:8-16.:8-16 Olsen JH, Hahnemann JM, Borresen-Dale AL, Tretli S, Kleinerman ... Correlates With The Number of Risk Alleles Int J Radiat Oncol Biol Phys 2007, 68:229-235 Lee KM, Choi JY, Park SK, Chung HW, Ahn B, Yoo KY, Han W, Noh DY, Ahn SH, Kim H, Wei Q, Kang D: Genetic...
... starting material Here, three fragment sizes were also chosen for the immunoprecipitation material, but only the 250- to 300-bp fragment size was chosen from the input The in-gel PCR was performed ... enzymes (GGT¥) Yuan et al *LDL, low density lipoproteins †ALT, alanine-aminotransferase ‡TC, total cholestrol §HDL, high density lipoproteins TG, triglycerides ¥GGT, gamma-glutamyl transferase for ... nuclei were resuspended in RIPA-buffer (1× phosphate-buffered saline (PBS), 1% NP-40, 0.5% Na-deoxycholate, 0.1% SDS, 0.004% Na-azide) for sonication with a Bioruptor (Diagenode, Liège, Belgium)...
... synthesis of choline occurs following the route phospho-ethanolamine (P-EA) to phospho-choline, P-choline (bold arrows, Fig 2) However, the route P-EA to phosphatidylcholine, Ptd-choline (normal ... phosphoethanolamine to phosphocholine, which is either dephosphorylated to form choline directly [92] or first incorporated into phosphatidylcholine and then metabolized to choline [93] (Fig 2) Primarily ... choline metabolites upon supply of 14C-choline demonstrated that choline import into chloroplast indeed limited its flux to glycinebetaine [99] Hence, it was postulated that a high-activity choline...
... preconditioning protects post-ischemic oxidative damage to mitochondria in rat liver Shock 2005, 24:370-375 15 Sun K, Liu ZS, Sun Q: Role of mitochondria in cell apoptosis during hepatic ischemia-reperfusion ... assessed by liver parameters, and we speculate that the explanation may be the same as above We choose the Knudsen et al Comparative Hepatology 2011, 10:3 http://www.comparative-hepatology.com/content/10/1/3 ... ischaemia exceeding h Br J Surg 1993, 80:1161-1165 10 Belghiti J, Noun R, Malafosse R, Jagot P, Sauvanet A, Pierangeli F, Marty J, Farges O: Continuous versus intermittent portal triad clamping...
... fluorescent proteins and their brightness in CHO cytoplasm 74 Figure 4.10 Model probabilities and model selection of fluorescent proteins in CHO cytoplasm at different excitation intensities ... different fitting models using EGFP measured in CHO cytoplasm 76 Figure 4.12 Model probabilities and model selection and of fluorescent proteins in CHO cytoplasm with different acquisition ... selection at 37 ° 78 C Figure 4.14 PMT-EGFP in CHO cells and its model probabilities 80 Figure 4.15 Inferred fitting parameters of PMT-EGFP on CHO membrane with different excitation intensities...