bulk transport across the plasma membrane occurs by exocytosis and endocytosis
Báo cáo khoa học: Modulation of P-glycoprotein-mediated multidrug resistance by acceleration of passive drug permeation across the plasma membrane potx
... chloroform and diethyl ether reversed the resistance by acceleration of the passive transport of the drug or dye across the plasma membrane At these concentrations, the anesthetics did not inhibit the ... in the plasma membranes [31], it seems that the anesthetics accelerate the permeation by enhancing the drug flip-flop rate across the lipid core of the membrane Benzyl alcohol, diethyl ether and ... result, and in correlation with inhibition of the active export of daunorubicin by Pgp (Fig 4) On the other hand, the general anesthetics chloroform, propofol, and diethyl ether, and the local anesthetic...
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Báo cáo khoa học: Protein transport in organelles: Protein transport into and across the thylakoid membrane pptx
... with the precursor–receptor complex and the putative translocase is formed (iii) The precursor protein is then transported in a process energized by the DpH across the thylakoid membranes The transported ... transport signal, which then mediates transport across the thylakoid membrane Once across the thylakoid membrane, this signal peptide is also cleaved off, this time by the thylakoid processing ... from the translocase into the lipid bilayer, where the signal peptide is removed by the TPP and the mature protein is released into the lumen After protein transport, Tha4 dissociates from the...
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Báo cáo khoa học: Protein transport across the endoplasmic reticulum membrane pdf
... the membrane by the signal recognition particle (SRP) The ribosome ⁄ SRP ⁄ nascent chain complex is bound to the membrane, first by an interaction between SRP and its membrane receptor (SR), and ... by its folding, the N-terminus can flip across the channel and subsequently exit laterally into the lipid phase When the N-terminus is retained in the cytosol and the polypeptide chain is further ... Protein transport across the ER membrane T A Rapoport complex, called the Sec61 complex in eukaryotes and the SecY complex in bacteria and archaea [2] The a-subunit forms the channel pore,...
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Báo cáo khoa học: Activation of the plasma membrane H+-ATPase of Saccharomyces cerevisiae by glucose is mediated by dissociation of the H+-ATPase–acetylated tubulin complex docx
... immersed in the plasma membrane It functions to hydrolyze ATP and to transport H+ out of the cell, thereby regulating internal pH An important finding was that glucose activates the plasma membrane ... determined by its H+-pumping or ATP hydrolyzing activity (Fig 1B) These rapid effects of d-glucose suggest action at the membrane level, possibly during the transport of glucose into the cell We therefore ... K+-ATPase in the plasma membrane of mammalian cells To confirm the interaction of acetylated tubulin with H+-ATPase and the presence of the H+-ATPase–acetylated tubulin complex in membranes of...
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báo cáo khoa học: "Trichoderma viride cellulase induces resistance to the antibiotic pore-forming peptide alamethicin associated with changes in the plasma membrane lipid composition of tobacco BY-2 cells" potx
... and MA conceived the study and planned the majority of the experiments MA conducted all the experiments HT and ASS took part in the lipid analyses and the interpretation of the results from these, ... strongly indicated by the reduction in elicited resistance by heat inactivation and by the presence of the cellulase inhibitor cellobiose Further, the effect of inhibitors excludes the possibility ... had not been exposed to T viride, and the situation is far from the soil situation where fungus and plant grow together and influence each other The objective of the present investigation was to...
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Tài liệu Báo cáo khoa học: Characterization of the interaction between the plasma membrane H+-ATPase of Arabidopsis thaliana and a novel interactor (PPI1) doc
... Fusicoccin binding to its plasma membrane receptor and the activation of the plasma membrane H+-ATPase IV Fusicoccin induces the association between the plasma membrane H+-ATPase and the fusicoccin receptor ... et al ([1] and references therein) The auto-inhibitory action of the C-terminus is suppressed, besides by pH, by lysophospholipids and by 14-3-3 proteins ([1] and references therein) The latter ... XL10 and the frame and the identity of the cloned fragment verified by sequencing The construct with the N-terminal portion of PPI1 has been previously described [22] The DNA fragment coding for the...
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Báo cáo khoa học: A kinetic approach to the dependence of dissimilatory metal reduction by Shewanella oneidensis MR-1 on the outer membrane cytochromes c OmcA and OmcB potx
... acceptor These results therefore provide evidence that there are differences between the electron transfer pathways towards chelated Fe(III) on the one hand and either U(VI) or Se(VI) on the other ... composed of cytochromes and a quinone, located in both the cytoplasmic membrane (CymA and menaquinone) and the outer membrane (OmcB, and a partial role for OmcA) [4,9–11] The 21 kDa tetraheme cytochrome ... 2007 The Authors Journal compilation ª 2007 FEBS J Borloo et al A B C D other Statistical analysis (Student’s t-test) between the MR-1R values and the sum of the values of the omcA– and the omcB–...
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Báo cáo khoa học: Critical role of the plasma membrane for expression of mammalian mitochondrial side chain cleavage activity in yeast pptx
... most of the antigen is detected in the plasma membrane, with a minor fraction localizing to the ER To determine whether the SCC reaction occurs in the PM, where most CYP11A1 is found, or in the ER, ... synthesis The well-characterized StAR protein is involved in the rapid transport of cholesterol to the inner mitochondrial membrane [11] In contrast, the factors and processes responsible for the ... cells in these respects The quantity of steroid produced is controlled by the availability and mobility of the substrate together with the concentration of Adxp Unexpectedly, Adxp, Adrp and CYP11A1...
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Báo cáo khoa học: Separation and characterization of caveolae subclasses in the plasma membrane of primary adipocytes; segregation of specific proteins and functions docx
... cytochrome oxidase and less than 7% of the cell Golgi TGN38 protein, indicating a very limited contamination by other membrane fractions of the purified plasma membrane [2] Purified plasma membranes were ... from the bottom of the tube, membranes were collected by centrifugation, and analysed by SDS ⁄ PAGE and immunoblotting for the distribution of caveolin (——), perilipin ( ), and SR-BI (– — –) The ... present in the VHD-caveolae with the same relation to caveolin-1 as in the HD-caveolae and LD-caveolae; (c) the VHD-caveolae contained almost a third of the plasma membrane caveolin, and the majority...
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Báo cáo khoa học: The antibody to GD3 ganglioside, R24, is rapidly endocytosed and recycled to the plasma membrane via the endocytic recycling compartment ppt
... recycled to the plasma membrane and released into the culture medium How can it be explained that R24 is not associated with the plasma membrane after recycling and release into the extracellular ... back to the plasma membrane in the absence of the crosslinker Alternatively, clathrin independent recycling pathways may also be involved in the recycling of molecules to the plasma membrane, ... receptor and its ligand transferrin recycle to the plasma membrane with the same kinetics as certain lipids [34] and independently of the transferrin receptor cytoplasmic domain [38] These data...
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Báo cáo khoa học: A hydrophilic cation-binding protein of Arabidopsis thaliana, AtPCaP1, is localized to plasma membrane via N-myristoylation and interacts with calmodulin and the phosphatidylinositol phosphates PtdIns(3,4,5)P3 and PtdIns(3,5)P2 pptx
... to the membrane in the tissues, and was present at 0.079% and 0.029% of total membrane proteins in the shoots and roots, respectively The stability of the interaction of PCaP1 with the plasma membrane ... the plasma memPCaP1 brane and cytosol, respectively (Fig 4C,D) The results indicate that the N-terminal part with 27 residues has the ability to localize the protein to the plasma membrane, and ... PtdInsPs The binding of PCaP1 to the plasma membrane was not affected by CaM, even in the presence of Ca2+ Therefore, CaM and Ca2+ may regulate the unidentified function of PCaP1 localized in the plasma...
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Báo cáo khoa học: Modulation of Ca2+ entry and plasma membrane potential by human TRPM4b pptx
... close to the nucleus, but also of small vesicular structures throughout the cell body and in the plasma membrane These data indicate de novo expression of the TRPM4–EGFP fusion protein and subsequent ... were then expanded in the same medium and analyzed for the expression of TRPM4b by RT-PCR and western blotting RT-PCR Total RNA was isolated, and RT-PCR was performed as described above The primer ... according to the manufacturer’s instructions Electrophysiology Membrane currents and membrane potentials were recorded in the whole cell and in the perforated-patch configuration of the patch clamp...
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Báo cáo khoa học: Role of the plasma membrane leaflets in drug uptake and multidrug resistance ppt
... outer leaflet of the plasma membrane The lipid core of the plasma membrane constitutes the main barrier to TMRM transport across the membrane and the mitochondria cannot affect the TMRM concentration ... could be the result of TMRM transfer from the cell surface further into the outer leaflet of the membrane or flip-flop across the membrane and residence in the inner leaflet of the membrane The observation ... that the limiting step in the release of dye from the plasma membrane is not the actual release from the membrane but, instead, the movement away from the plasma membrane into the cell The cytoplasm...
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báo cáo khoa học: " The PTI1-like kinase ZmPti1a from maize (Zea mays L.) co-localizes with callose at the plasma membrane of pollen and facilitates a competitive advantage to the male gametophyte" doc
... in bold and of less appearance in subscribed letters plasma membrane by N-terminal acylation, likely myristoylation To study the structural basis of ZmPti1a being targeted to the plasma membrane ... to membranes Combined replacement of both adjacent residues in either one of the two motifs seems to strengthen mistargeting and completely prevents ZmPti1a membrane association ZmPti1b, c and ... i.e top, middle and bottom of individual cobs The severeness of fungal infection in these samples was monitored based on the visual rating using the silk channel scale [24] and by RT-PCR detection...
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The plasma membrane lipid rafts caveolae mediated PACAP signalling in PC12 cells
... oligomerization and the interaction with other proteins (Li et al 1996a) The membrane- spanning domain (MS) forms a hairpin-like loop within the membrane, thereby exposing both the amino- and carboxyl-termini ... invaginations of the plasma membrane characterized by the presence of the caveolin protein (Anderson 1998) It has been suggested that lipid rafts act in the sorting and subsequent transport of sphingolipids, ... husband for their dedicated love, confidence, support, encouragement, understanding and patience to stand by me throughout my candidature This thesis is consecrated to them with my sincere and deepest...
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Control of Membrane Fouling by Coagulant and Coagulant Aid Addition in Membrane Bioreactor Systems
... PSI-100, and PSI-025 It suggested that the PSI had affected and coagulated the SMP in the reactors and prevented the membrane from fouling From these results, it can be stated that in the case ... washed (physical washing by tap water while wiping the membrane with hand or chemical washing by submerging the membrane in 0.25% NaOCl solution for to h) when the pressure of the suction pump decreased ... concentrations and membrane fouling in MBR The reduction of membrane fouling by PSI addition was caused by the coagulation of SMP ACKNOWLEDGEMENTS The authors would like to thank Mr N Shimada and Mr...
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Guidance on the teaching of writing skills INSET opportunities for teachers of a subjects across the curriculum at Key Stages 2 and 3 docx
... and the skills framework and/ or provide guidance for teachers of other subjects across the curriculum? What outcome will there be? Which of these (or other) success criteria are relevant to the ... reopen the town youth club It was closd over the sumer and so the young pepl and childrin home in the holidays couldnt find anything to They where rude to the peple in the town becaus they was ... that they can: • engage their reader(s) • adapt their writing to suit the audience and purpose of the piece • use grammatical and stylistic features to ensure clarity, achieve the right tone and...
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Indian and Northern Affairs Canada Internal Audit Report Financial Audit of the Settlement Allotment Prepared by: Audit and Assurance Services Branch April 9, 2009 pptx
... disbursement of the Settlement Allotment funds and compliance with the terms of the TB decision and applicable INAC and TB policies The scope of the audit included an overall assessment and detailed ... 2006/07 and $186.4M in 2007/08 Objectives and Scope The objective of the financial audit of the Settlement Allotment was to provide assurance on the adequacy and effectiveness of controls over the ... over the disbursement of the Settlement Allotment funds In our opinion, the charges to the Settlement Allotment for the fiscal years 2006/07 and 2007/08 are accurate, complete and comply with the...
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Báo cáo y học: "Infiltration of the synovial membrane with macrophage subsets and polymorphonuclear cells reflects global disease activity in spondyloarthropathy" pps
... Oss, The Netherlands Authors' contributions DB, EMV, and FDK designed the study DB, EK, and LDR sample and analyzed the synovial tissues HM selected the patients DB collected and analyzed the ... differences between PsA on the one hand and AS and USpA on the other hand (unpublished data), both unsupervised and supervised clustering analysis of the present data indicated that the synovial heterogeneity ... mAb 12A was provided by AMB DB, LDR, and AMB prepared the manuscript, and EMV, HM, and FDK reviewed it All authors read and approved the final manuscript Acknowledgements The authors wish to thank...
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Báo cáo y học: " Expansion of the human mitochondrial proteome by intra- and inter-compartmental protein duplication" ppsx
... ([GO:5975], P < 2e-4) and various components of transport ([GO:6810], P < 6e-4, amino acid transport, ion transport and protein transport complexes embedded in the inner and outer membranes) Additionally, ... duplications expanded the metazoan and human mitochondrial proteome by the duplication of non-mitochondrial proteins and redirecting the newly arisen gene products to the mitochondria The two modes ... responsible for the binding with Ca2+, as the binding site is created upon the formation of a complex with the E2 subunit of the PDH complex and requires the lipoyl groups of E2 [44] Nevertheless, the calcium-dependence...
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