... all ofthe OLP epithelium. The count was performed by two investigators without knowledge of whether the samples were control or experimental. The total number of basal and suprabasal epithelial ... Negative controls consisted of omission ofthe primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead ofthe primary antibody. Cell quantification ... OLP would require an increased immunolocalization of hMSH2. Therefore, the reduced expression of hMSH2 protein could make the epithelium of OLP more susceptible to DNA mutation, making it prone...
... respectively, of that of HPRT. This low activitycould be explained by the fact that PRTFDC1 has a Gly in the position of the proposed catalytic Asp of HPRT. In PRTFDC1, a water molecule at the position of ... spectrophotometric methods.Because the enzymatic activity of PRTFDC1towards hypoxanthine and guanine is only 0.26% and0.09%, respectively, ofthe activity of HPRT, the role of the PRTFDC1 in purine metabolism ... activity [10]. The structural characterization of numerous com-plexes ofthehuman HPRT and several bacterial andprotozoan HPRTs have been undertaken [13–17]. The structure ofhuman HPRT can...
... permonomer of poly(dT), showing that the changes weremainly due to the binding ofthe first DNA, while the binding ofthe second DNA had less influence(Fig. 5A). To ensure that the estimation ofthe ... bymutations on the DNA-binding site of Rad51, suggest-ing that the L2 loop of Rad51 is not far from the DNA-binding site. Consistent with this idea, the fluor-escence ofthe tryptophan inserted in the ... 1).These results confirm that the residues are close to the DNA-binding site.We then examined if these fluorescence changesoccurred by the binding ofthe first or second DNA,by titrating these...
... encoded another type of KLK11, isoform 2,despite the presence of exon 1c. The secretory path-ways of KLK11 isoforms appeared to differ, althoughboth isoforms were secreted from cells. Hence, the ... show the transcription initiation sites ofthehuman and a white arrowhead shows the transcription initiation site of the mouse. Dashes show gaps and asterisks show the same nucleotide in the ... mechan-ism of KLK11 gene expression.ResultsThree different primary transcripts are derivedfrom thehuman KLK11 geneWe determined the nucleotide sequences of 15 clones of the PCR products from the human...
... and phylogeny. How-ever, the nature ofthe connections between thesefactors needs to be understood at the level ofthe pro-tein domain. The global properties ofthe HTFNtopology are partially ... property of the bHLH domains. Therefore, this seems to be a topo-logical constrain derived from the evolution of thisfamily.Evolution based on domain reusing might explain the abundance of certain ... TFIIE-a, TFIIE-bF 57 42% Zn finger domains. It contains the 90% of the members of nuclear receptorsuperfamily (they are Zn fingersalso) ofthe HTFN.14-3-3 zeta, STAT1a, STAT1b, dCREB, ATF-1,FTF,...
... cells. The positions ofthe molecular size markers (kDa) are indicated to the left and right ofthe (C) and (D),respectively, whilst the position ofthe Oct-1 (98 kDa approximately) and the two ... (2002) Characteri-zation ofthe 5Â untranslated region of alpha and betaisoforms ofthehuman thromboxane A2 receptor (TP).Differential promoter utilization by the TP isoforms.Eur J Biochem 269, ... (Prm)3within thehuman TXA2receptor (TP) gene thatdirects expression of TPb in HEL92.1.7 and HEK293cells [25]. A schematic ofthehuman TP gene highlight-ing the positions ofthe previously...
... to the protein of interest. In the case ofthe rpL32 fragmentor the (GCC)9sequence, there are several repeatedZF5-binding sites within a single oligonucleotide.Therefore, the affinity of ... repressor of thehuman FMR1 gene. Thus, these data identify a new class of ZF5targets, a subset of genes containing GCC-elements in their regulatoryregions, and raise the question of whether transcription ... (1994)Identification of a GC element in the promotor of the murine L32 ribosomal protein L32 gene and the 3Â-ter-minal region ofthehuman apolipoprotein A-I gene,capable of binding with murine and human...
... epitopes locatedin the MUC5AC C-terminal cysteine-rich part.Further localization ofthe epitopes of 45M1,2-12 M1 and 166M1In order to further locate the epitopes ofthe hithertounmapped 45M1, ... detailedmap ofthe epitopes for the mAbs, where 45M1 recog-nizes an epitope located in the N-terminal region of the C-terminal cysteine-rich part of MUC5AC, pre-sumably in the last CysD domain ofthe ... 5. Mapping ofthe 45M1, 166M1 and 2-12M1 epitopes on the C-terminal cysteine-rich part ofhuman MUC5AC. The upper part of the figure shows a schematic representation of full-length human MUC5AC...
... subunit of Pph3p required for the binding of Psy2p, we examined the sensitivity ofthe YBL046w ⁄ Psy4p deletion strainto cisplatin. The comparable increased sensitivities tocisplatin ofthe homozygous ... was the same as that ofthe controlstrain transformed with the vector YCplac111 (datanot shown). The YBL046w gene was therefore termedPSY4 (for platinum sensitivity 4). The primary target of ... Superose6 at the same anomalous position of 450 kDa as Dro-sophila His6–R2 (Fig. 3) and human His6–R2 [16].Examination ofthe phenotype of S. cerevisiaestrains carrying a deletion ofthe YBL046w...
... per-centages ofthe cells in the G0 ⁄ G1, S and G2 ⁄ M phases ofthe cellcycle after treatment with 20 lM or 100 lM ATP for 24 h. The gat-ing used for the quantification ofthe cells in the G0 ⁄ ... completelyreversed the inhibition of LXF-289 cell proliferationby ATP. Further indirect support for the involvement of ERK1 ⁄ 2 is derived from the observed translocation of ERK1 ⁄ 2 to the nucleus. The translocation ... detectabledecrease ofthe p50 form of NF-jB1 in the cytosol after10 min, which is still visible after 60 min (Fig. 5D).Simultaneously with the decrease of p50 in the cytosol,an increase of NF-jB1 p50 in the...
... late steps ofthe process. For example, early in the NER process, RPA assists TFIIH in the opening of the DNA helix around the damage site [36]. Further-more, in the presence ofthe XPA minimal ... sites.Taken together, these findings suggest that the struc-tural properties of DNA-damaged substrates, whetherintrinsic or the result of protein binding, function in the recruitment ofthe XPC–hHR23B ... Binding of XPG induces the release of XPC–hHR23B, whereas XPF–ERCC1 triggers exci-sion ofthe damaged DNA and the release of XPAand TFIIH. Subsequently, the newly formed gap in the DNA is filled by...
... in front ofthe HSVtk promoter and transfected into HeLa cells.CAT activity was normalized for transfection efficiency. The values of the activities are set relative to the activity ofthe clone ... mL of sample. The column was eluted at0.1 mLặmin)1. Fractions of 0.5 mL were collected after the void volume ofthe column.SDS/PAGE and protein identification by MALDI-TOF MSSamples from the ... identifica-tion of NF1 by MALDI-TOF MS is based primarily onpeptide mass matches within the conserved DNA bindingdomain, we cannot distinguish between the variousisoforms. Furthermore, NF1 exist in the...
... localization in the ER [19]. We found that the TMD of UGT1A pro-teins appended to the C-terminus ofthe ectodomain of CD4 plasma membrane glycoprotein was able to retain the chimeric protein in the ER ... that disruption ofthe dilysinemotif KSKTH of UGT1A by mutation of lysine toserine residues or by extending the length of the cytoplasmic tail to relocate the dilysine from the crit-ical positions ... together, these data indicated that the TMD domain of UGT1A was sufficient to retain the ectodomain of CD4 protein in the ER. Because the carbohydrate moieties of proteins that are trans-ported to the...
... of the 15N-1H NOEs were obtainedfrom the difference of two independent experiments. The values of error were adjusted for the proper tensor2 analy-sis, by increasing ofthe errors from the ... further our knowledge withrespect to the mechanisms of action of these proteins,it is crucial that we define the precise boundaries of the STI1-homologous domain and compare the struc-tures of ... characteristics ofthe 275–284 segment of hHR23B. Because ofthe high quality of our15N-HSQC spectra, 54 ofthe 58 protonated backbonenitrogen atoms were available for relaxation measure-ments. The values...
... independent of 15d-PGJ2stimulation.Thereafter, the precise site of action of 15d-PGJ2was identified by further 5Â deletion analysis dissecting the )192 to )154 region of Prm3. Deletion of the PPARc ... (DR5). These dataprovide direct evidence for the role of PPARc in the regulation of human TP gene expression within the vasculature and point to further critical dif-ferences in the modes of transcriptional ... 15d-PGJ2-suppres-sion of luciferase expression. On the other hand, eitherdeletion ofthe latter PPARc (b) and RXR IV halfsites, such as within the )154 subfragment, or dis-ruption ofthe RXR IV half...