... osteogenic differentiationofhumanmesenchymalstemcells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiationofhuman ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymalstem cells: regulation of niche, ... differentiationof rat bone marrow-derived mesenchymalstemcells J Bone Miner Res (10):16061614 59 Matsubara T, Tsutsumi S, Pan H, et al 2004;A new technique to expand humanmesenchymalstemcells using...
... number ofcells was determined spectrophotometrically entiation ofhumanmesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiationofhumanmesenchymalstem ... Kassem M: Inhibition of osteoblast differentiation but not adipocyte differentiationofmesenchymalstemcells by sera obtained from aged females Bone 2006, 39:181-188 Page of (page number not ... Lecanda F, Avioli LV, Cheng SL: Regulation of bone matrix protein expression and induction ofdifferentiationofhuman osteoblasts and human bone marrow stromal cells by bone morphogenetic protein-2...
... vitro differentiationof size-sieved stemcells into electrically active neural cellsStemCells 2002, 20(6):522-529 Tzeng SF, Tsai MJ, Hung SC, Cheng H: Neuronal morphological change of size-sieved ... promote differentiationofhumanmesenchymalstemcells into insulin producing cells J Biomed Mater Res A 2008, 86(4):1097-1105 Chao KC, Chao KF, Fu YS, Liu SH: Islet-like clusters derived from mesenchymal ... 53(7):1721-1732 Page 10 of 10 doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiationofhumanmesenchymalstemcells into insulin producing cells through...
... NANOFIBERS FOR OSTEOGENIC DIFFERENTIATIONOFHUMANMESENCHYMALSTEMCELLS NGUYEN THI HIEN LUONG (B.Eng., Ho Chi Minh city University of Technology, Vietnam) A THESIS SUBMITTED FOR THE DEGREE OF ... Nguyen, Susan Liao, Seeram Ramakrishna and Casey K Chan Role of Nanofibrous Structure in Osteogenic DifferentiationofHumanMesenchymalStemCells with Serial Passage Nanomedicine 6(6): 961-974, 2011 ... Ramakrishna Electrospun Poly(L-lactic acid) Nanofibres Loaded with Dexamethasone to Induce Osteogenic DifferentiationofHumanMesenchymalStemCells Journal of Biomaterials Science, Polymer Edition...
... expression pattern of undifferentiated and differentiated human embryonic stemcellsStemCells Dev 2007, 16(6):1003-1016 Page 15 of 17 (page number not for citation purposes) Journal of Translational ... The tested adult cells were Human Microvascular Endothelial Cells (HMVEC), Human Umbilical Vein Endothelial Cells (HUVEC), Umbilical Artery Smooth Muscle Cells (UASMC), Normal human astrocytes ... 13 The expression of miR-92 has been reported in human embryonic stem (ES) cells [16,26], mouse ES cells[ 20] or Page 12 of 17 (page number not for citation purposes) Journal of Translational Medicine...
... culture of ICM cells with stem cell-like morphology from human blastocysts (Fig 1.1A) though cultures failed beyond passages The first long-term culture (4-5 months) ofhuman embryonic stemcells ... analysis of developmental events was made possible with the isolation of embryonic stemcells Extensive investigation of the properties and capabilities of these cells has led to the development of ... Mu ° – Degree x CHAPTER 1: GENERAL INTRODUCTION 1.1 Embryonic stemcells The isolation of mouse and human embryonic stemcells (ES cells) heralded a new era in regenerative medicine, raising hopes...
... the induction of MSC adipogenesis Myogenesis Most investigations of myogenesis in adult stemcells are based on a small population of skeletal muscle-derived stem cells, or satellite cells A recent ... is critical for self-renewal ofhuman multipotent adipose-derived stemcellsStemCells 2006, 24:2412-2419 Kleber M, Sommer L: Wnt signaling and the regulation ofstem cell function Curr Opin ... homing ofhumanmesenchymalstemcells at exposed sites but promotes their widespread engraftment to multiple organs: a study of their quantitative distribution after irradiation damage Stem Cells...
... Spontaneous differentiationof germ cells from human embryonic stemcells in vitro Human molecular genetics 2004, 13:727-739 29 Niwa H, Miyazaki J, Smith AG: Quantitative expression of Oct-3/4 defines differentiation, ... RT-PCR analysis of expression levels of hTERT and GAPDH, and (B) Real-time RT-PCR analysis of expression levels of DNMT1, DNMT3A and DNMT3B in primary humanmesenchymalstemcells transfected ... ofmesenchymalstem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stemcells from human bone marrow Stem...
... Osteogenic Cells 46 2.8.2.2 MesenchymalStemCells 48 2.8.2.3 MesenchymalStemCells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal MesenchymalStemCells ... Chong, Z Zhang, M Choolani, J Chan Human endothelial progenitor stemcells enhances osteogenic response of bone marrow derived human fetal mesenchymalstemcells through paracrine signalling ... Chong, Z Zhang, MA Choolani, SH Teoh Coculture ofhuman endothelial progenitor stemcells and bone marrow-derived human fetal mesenchymalstemcells potentiates osteogenesis through paracrine...
... xeno-free cryopreservation method for the storage ofhuman embryonic stemcellsStemCells 2004;22(5):779-89 10 Speicher MR, Ballard SG, Ward DC Karyotyping human chromosomes by combinatorial multi-fluor ... Embryonic stem cell lines derived from human blastocysts Science 1998;282(5391):1145-7 Reubinoff BE, Pera MF, Fong CY, Trounson A, Bongso A Embryonic stem cell lines from human blastocysts: somatic differentiation ... bioreactors Conflicts of interest The authors have declared that no conflict of interest exists References Becker EW The roles of ATP in the dynamics of the actin filaments of the cytoskeleton...
... erythroid differentiationof murine ES cells in vitro In these experiments we utilized the ES ⁄ OP9 cell in vitro differentiation system described by Kitayima et al [20] The ability of these cells ... Transcription of LCR hypersensitive site in undifferentiated murine embryonic stemcells and in human CD 133+ bone marrow cells (A) Transcription of LCR regions and the bmaj-globin gene during differentiation ... locus during the differentiationof hematopoietic stemcells to erythroid cells Transcription of HS3 could be important in maintaining this accessible structure, particularly in light of the fact...
... culture, expansion and manipulation ofhuman pluripotent stemcells The culmination of two events has enabled the production ofhuman embryonic stem cells: the birth of the first IVF test-tube baby ... summarizing 12 years of our team’s experience, skill and knowledge in the derivation, culture and expansion ofhuman embryonic stem cells, and more recently, ofhuman induced pluripotent stemcells The ... translational research in the field of embryonic and induced pluripotent stemcells It commences with practical aspects of the derivation and growth ofhuman embryonic stemcells from inner cell mass...
... marrow lipid was a consequence of the adipogenesis of marrow mesenchymalstemcells (MSCs) [7] Results of a previous study showed increased number of small size fat cells in the early steroid-associated ... trabecular bone area); fat cells diameter = the total diameter of fat cells in selected field/the number of fat cells in selected field; fat cells area = the area of all fat cells in selected field/(the ... steroid-associated ON, that might be derived from the adipogenicdifferentiationof MSCs [8] The in vitro studies also showed elevated adipogenicdifferentiation ability of MSCs after steroid treatment [9,10]...
... epigenomic profiling of a cohort ofhuman embryonic stem cell (hESC) lines can be used to generate hESC reference epigenomes (left panels) The extent of reprogramming and differentiation potential of individual ... embryonic stem cell; H3K4me1, methylation of lysine of histone H3; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine ... stem cell; hESC, human embryonic stem cell; H3K4me3, trimethylation of lysine of histone H3; H3K27ac, acetylation of lysine 27 of histone H3; H3K27me3, trimethylation of lysine 27 of histone H3;...
... of 11 Mesenchymalstem cell treatment has no effect on the development of collagen-induced arthritis Figure Mesenchymalstemcells (MSCs) inhibit the anti-CD3induced proliferation of CD4+ T cells ... Page of 11 Figure Mesenchymalstemcells (MSCs) inhibit the proliferation of CD4 + T cells in vitro by induction of nitric oxide and prostaglandin E2 (PGE2) (a) CD4+ T cells, in the presence of ... immunomodulatory activity ofhuman bone marrow mesenchymalstemcellsStemCells 2006, 24:386-398 Meisel R, Zibert A, Laryea M, Gobel U, Daubener W, Dilloo D: Human bone marrow stromal cells inhibit allogeneic...
... Induction of intervertebral disc-like cells from adult mesenchymalstemcellsStemCells 2005, 23:403-411 23 Richardson S, Hughes N, Hunt J, Freemont A, Hoyland J: Humanmesenchymalstem cell differentiation ... A, Sekiya I: Increased proliferation ofhuman synovial mesenchymalstemcells with autologous human serum: comparisons with bone marrow mesenchymalstemcells and with fetal bovine serum Arthritis ... http://arthritis-research.com/content/12/6/R206 Page of 13 Figure Synovial MSCs affect gene profile of nucleus pulposus cells in co-culture system (a) Morphology of mono-culture of rat nucleus pulposus cells and human synovial MSCs...
... specific diseases in the course of ex vivo gene therapy 1.1.1 Human Embryonic StemCellsHuman embryonic stem (hES) cells are cells derived from the inner cell mass ofhuman blastocysts The hES cell ... MODIFICATION OFHUMAN EMBRYONIC STEMCELLS DU JUAN (B Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY DEPARTMENT OF BIOLOGICAL SCIENCES NATIONAL UNIVERSITY OF SINGAPORE AND INSTITUTE OF ... BACULOVIRAL TRANSDUCTION OFHUMAN EMBRYONIC STEMCELLS 11 2.1 Introduction hES cells hold great potential as a system for the study of lineage specification and as an unlimited source ofcells for the regenerative...
... Adult stemcells Adult stemcells also known as somatic stemcells can be found in diverse tissues and organs The most common adult stemcells are the hematopoietic stem cells, mesechymal stemcells ... blood cells and platelets, while skin stemcells give rise to the various types of skin cells Some recent reports suggest that adult stemcells such as haemopoietic stem cells, neuronal stemcells ... ofcells make up the human body viz., germ cells, somatic cells and stemcells Somatic cells include the bulk of the cells that make up the human adult and each of these cells in their differentiated...