activator of akt signaling
Computational modeling of cell signaling dynamics hypothesis management and parameter estimation methods applied to the akt pathway
... FOR AKT ACTIVATION 88 Page VII Non-canonical causes of altered Akt localization 88 Non-canonical causes of altered Akt phosphorylation dynamics 89 Systematic generation of alternative ... activation of membrane Aktp308 A hypothesis management analysis of five potential non-canonical mechanisms of augmented Akt phosphorylation supported the following hypotheses: augmented Akt recruitment ... 64 4.2 AKT ACTIVATION PATHWAY 66 4.3 MOTIVATIONS FOR MODELING DYNAMICS OF AKT PHOSPHORYLATION UPON ACTIVATION BY GROWTH FACTORS 67 4.4 REDOX REGULATION OF AKT ACTIVATION...
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Evolution of VoIP Signaling Protocols
... working with them to enhance the features of SIP servers so that they can have the functionality of a softswitch Such a softswitch may perform the functions of a call controller, MGC, and SS7 SG ... with the CC using IP links The softswitch concept probably originated during the development of MGCP A softswitch is sometimes referred to as a collection of softwaredefined entities residing ... features of SCTP are (a) backward compatibility with UDP, (b) acknowledged, error-free, and nonduplicated transfer of user data, (c) sup- 42 EVOLUTION OF VoIP SIGNALING PROTOCOLS port of data...
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Tài liệu Báo cáo khoa học: miRNAs and regulation of cell signaling pptx
... of complex regulatory networks Model of regulatory networks in the (A) MAPK signaling pathway, (B) Notch signaling pathway, (C) EcR signaling pathway, (D) Hippo signaling pathway, (E) TGF-b signaling ... levels of Hh signaling identified miRNAs that had been downregulated Some of these miRNAs (miR-125b, miR-326 and miR-324-5p) target activator components of the Hh signaling pathway and suppress Hh signaling, ... dme-miR-8 Wnt signaling pathway Fig Involvement of miRNAs in various signaling cascades Many miRNAs are under the control of various conserved signaling pathways and in turn regulate components of these...
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Tài liệu Báo cáo khoa học: Myristoylation of the dual-specificity phosphatase c-JUN N-terminal kinase (JNK) stimulatory phosphatase 1 is necessary for its activation of JNK signaling and apoptosis pdf
... the phosphorylation of the p46 isoform of JNK, whereas sorbitol treatment induced phosphorylation of both the p46 and p55 isoforms To confirm functional activation of the JNK signaling pathway, ... MAPK signaling, JSP1 was shown to be a positive regulator of the JNK signaling pathway [20,21] Since myristoylation determined the subcellular localization of JSP1, we asked whether abrogation of ... intrinsic phosphatase activity of the enzyme However, myristoylation determined the subcellular localization of JSP1, and was required for JSP1-induced activation of the JNK signaling pathway When overexpressed,...
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Tài liệu Báo cáo khoa học: Hsp105b upregulates hsp70 gene expression through signal transducer and activator of transcription-3 pdf
... Hsp105b-induced expression of the hsp70 gene Nuclear localization of Hsp105b is necessary but not sufficient for activation of the hsp70 promoter The promoter region between )206 and )187 bp of the hsp70 gene ... stimulates the phosphorylation of STAT3 at Tyr705 and translocation into the nuclei of cells Fig The region between amino acids 642 and 662 of Hsp105b is necessary for activation of the hsp70 promoter ... Hsp105b (A) Schematic representation of a series of C-terminal deletion mutants of Hsp105b The asterisk in the Hsp105bmNLS mutant indicates the location of the mutated NLS sequence (B) The expression...
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Tài liệu Báo cáo khoa học: Efficient killing of SW480 colon carcinoma cells by a signal transducer and activator of transcription (STAT) 3 hairpin decoy oligodeoxynucleotide – interference with interferon-c-STAT1-mediated killing pdf
... Interferon-gamma-induced sensitization of colon carcinomas to ZD9331 targets caspases, downstream of Fas, independent of mitochondrial signaling and the inhibitor of apoptosis survivin Clin Cancer ... inhibits the transcriptional activity of STAT3 and colocalizes with STAT3 to the cytoplasm of SW480 cells The transcriptional activity of STAT3 after treatment of the cells with hpST3dODN was analysed ... possible effects as a result of the introduction of DNA within cells, and indicating that, in itself, the interaction of ODN with STAT3 induced these effects (i.e inhibition of the cyclin-D1-dependent...
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Báo cáo khoa học: ATP-binding domain of heat shock protein 70 is essential for its effects on the inhibition of the release of the second mitochondria-derived activator of caspase and apoptosis in C2C12 cells potx
... role of HSP70 in the inhibition of the release of Smac and apoptosis GAPDH Ratio of HSP70 to GAPDH 14 # 12 # 10 * The role of the ATP-binding domain of HSP70 in the prevention of the release of ... domain of HSP70 is essential for the inhibition of H2O2-induced activation of caspases-9 and -3 and apoptosis (A) The effects of HSP70 and its deletion mutant proteins on the activation of caspases-9 ... inhibition of Smac release from mitochondria, and the suppression of caspase activation Such a mechanism is independent of the interaction of HSP70 with Smac but requires the ATP-binding domain of the...
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Báo cáo khoa học: Modulation of the Arabidopsis KAT1 channel by an activator of protein kinase C in Xenopus laevis oocytes potx
... shown to phosphorylate the C-terminal region of KAT1 [16] One of the 10 members of the SNF1-related protein kinase in A thaliana, SnRK2.6, is an ortholog of AAPK and shares 79% amino acid identity ... T308 in KAT1 Modification of T306 abolished KAT1 activity in oocyte recordings, whereas modification of T308 did not cause a loss of function [21] In animal cells, one type of Ser ⁄ Thr protein kinase, ... Percentage of current inhibition 32 after the addition of PMA the program prosite (http://ca.expasy.org/prosite/) to predict residues of KAT1 that might be phosphorylated by PKC All of the resultant...
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Báo cáo khoa học: A novel splicing variant form suppresses the activity of full-length signal transducer and activator of transcription 5A pot
... 3¢-end, resulting in the production of b-isoforms truncated at the transactivation domain STAT5A b-isoforms and STAT5B b-isoforms are generated by insertion of intron 18, and lack only the transactivation ... characterization of the intermediate isoform of the human prolactin receptor J Biol Chem 274, 35461– 35468 6322 16 Fang F, Antico G, Zheng J & Clevenger CV (2008) Quantification of PRL ⁄ Stat5 signaling ... activation of PRL-stimulated STAT5A to 2.2-fold (Fig 7C) The expression of STAT5A_DE18 suppressed the PRLinduced activity of STAT5A by 33% Furthermore, we observed nuclear translocation of STAT5A_FL...
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Báo cáo khoa học: A designed curved DNA segment that is a remarkable activator of eukaryotic transcription potx
... Designed DNA as an activator of transcription Fig Demonstration of the absence of the T20 segment in control cell lines The target region for PCR amplification is illustrated at the bottom of the figure ... contain a single copy of linearized pLHC20 ⁄ loxP ⁄ TLN-6, and the other two contain a single copy of linearized pLHC20 ⁄ loxP ⁄ -2 ⁄ TLN-6 By Fig Southern blot analysis of genomes of the established ... Authors Journal compilation ª 2006 FEBS 5695 Designed DNA as an activator of transcription N Sumida et al Fig Optimization of the position of the T20 segment, as examined in a transient transfection...
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Báo cáo Y học: A new high affinity binding site for suppressor of cytokine signaling-3 on the erythropoietin receptor potx
... down-regulation of the Epo signaling [20] In our experiments, we confirm binding of SOCS-3 to pY401, with a calculated Kd for this interaction in the range of 9.5 lM (Table 1) Concerning EpoR signaling, ... phosphotyrosine pY612 of the common b chain in the context of IL-3 signaling [45] A common feature of SHP1 and SHP2 recruiting motifs is a hydrophobic residue at position Y)2 of the binding phosphotyrosine ... mgÆmL)1) at a flow rate of 20 lLÆmin)1 for For measurement of the Kd value the flow rate was enhanced to 100 lLÆmin)1 in order to obtain higher resolution of kinetics For this type of experiment SOCS-3...
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Báo cáo khoa học: Hepatocyte growth factor activator is a serum activator of single-chain precursor macrophage-stimulating protein potx
... a major serum activator of pro-MSP Results Processing of pro-MSP by HGFA The effect of recombinant human HGFA was tested on the processing of recombinant human pro-MSP Incubation of pro-MSP with ... chain of MSP and the anti-His tag IgG recognized the poly-His tag at the C-terminus of MSP The N-terminal amino acid sequence of a product of approximately 30 kDa is indicated (B) Effects of NaCl ... of pro-MSP by HGFA (A) Immunoblot analysis of proteolytic cleavage of a His-tagged human pro-MSP recombinant protein by human HGFA Pro-MSP at a concentration of nM was incubated with 0.5 nM of...
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Báo cáo khoa học: Synergistic co-operation of signal transducer and activator of transcription 5B with activator protein 1 in angiotensin II-induced angiotensinogen gene activation in vascular smooth muscle cells potx
... treatment of VSMCs with Ang II for different periods of time, AGT mRNA was detected using RT-PCR As shown in Fig 1A, the level of AGT mRNA peaked h after stimulation with Ang II, showing an increase of ... the expression of the AGT gene in VSMCs stimulated with Ang II, the effect of Ang II on the phosphorylation of STAT5 and c-Jun was measured Ang II stimulated the phosphorylation of STAT5B and ... DNA-binding activity of STAT5B and of AP-1 increased in a time-dependent manner The specificities of two DNA–protein complexes were demonstrated by their disappearance upon the addition of a 100-fold...
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Báo cáo sinh học: " The involvement of survival signaling pathways in rubella-virus induced apoptosis" ppt
... and The phosphorylation of GSK-3β, downstream of Akt, increased from 12 and 96 hours p.i and was similar to that of Akt Phosphorylation of BAD, another substrate for Akt, however, could not be ... apoptosis Analysis of phosphorylation profiles during RV infection demonstrated that the presence of the virus stimulated an increase in the phosphorylation of ERK1/2, Akt, and Akt target GSK-3β ... pattern of downstream target p70S6K did not follow that of Akt and ERK1/2 Apart from being phosphorylated by ERK1/2 and mTOR/FRAP downstream of Akt, p70S6K can be phosphorylated by an array of different...
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báo cáo hóa học: " A role for DNA-dependent activator of interferon regulatory factor in the recognition of herpes simplex virus type 1 by glial cells" pot
... plaque assay of serial dilutions of HSV1 on Vero cells at 34°C MHV-68 viral stocks were prepared by infecting monolayer cultures of BHK-21 cells (ATCC, CCL-10) at a low viral MOI of 0.1 PFU per ... brains of infected animals (Figure 3B) in the absence of significant increases in levels of this viral sensor in total brain protein isolates (Figure 3A) Finally, we assessed the expression of the ... development of severe encephalitis that is associated with a high degree of morbidity and mortality While acyclovir is currently employed in the treatment of HSV encephalitis, drug-resistant strains of...
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báo cáo hóa học: " Pharmacological inhibition of Akt and downstream pathways modulates the expression of COX-2 and mPGES-1 in activated microglia" potx
... binds to the pleckstrin homology domain of one of the Akt (also known as protein kinase B) isoforms and facilitates the phosphorylation of Akt1 , Akt2 or Akt3 at Thr308/309/305 and Ser273/474/472, ... production of prostaglandin (PG) E2 and PGD2 Findings We show that inhibition of Akt by the Akt inhibitor X enhanced the production of PGE2 and PGD2 without affecting the expression of COX-2, ... pattern of differential expression between these two enzymes [5] To further investigate the contribution of pathways downstream of PI3K, we inhibited Akt with Akt inhibitor X As shown in Fig 1, Akt...
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Báo cáo hóa học: " Respiratory syncytial virus (RSV) attachment and nonstructural proteins modify the type I interferon response associated with suppressor of cytokine signaling (SOCS) proteins and IFN-stimulated gene-15 (ISG15)" potx
... specificity of the detection antibody for these isoforms, detection of IFNα is limited Moreover, low levels of type I interferon protein expression would be predicted in part because of the transient ... immunity [26] Production of type I IFN depends on activation of IRF3 and IRF7 [20,35,44] where type I IFN expression is negatively regulated by suppressor of cytokine signaling (SOCS) proteins ... induced by stimulation of TLRs where they not interfere with direct TLR signaling, but instead regulate paracrine IFN signaling [7] The SOCS protein family is comprised of eight proteins (CIS,...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" doc
... reference for the relative quantification of the gene of interest, which was expressed as the ratio of ‘concentration of the target’ to ‘concentration of b-actin’ Western Blot MG-63 cells were ... therapeutic options List of Abbreviations used CGRP: calcitonin gene-related peptide CGRP; OPG: osteoprotegerin; RANK: receptor activator of nuclear factor-B; RANKL: receptor activator of nuclear factorB ... ratio of 1:100 and (C) a cell-particle ratio of 1:500 Time course of UHMWPE particle-induced OPG mRNA expression after treatment with different alpha-CGRP doses in (E) a cell-particle ratio of 1:100...
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báo cáo hóa học:" Effects of alpha-calcitonin gene-related peptide on osteoprotegerin and receptor activator of nuclear factor-B ligand expression in MG-63 osteoblastlike cells exposed to polyethylene particles" pot
... reference for the relative quantification of the gene of interest, which was expressed as the ratio of ‘concentration of the target’ to ‘concentration of b-actin’ Western Blot MG-63 cells were ... therapeutic options List of Abbreviations used CGRP: calcitonin gene-related peptide CGRP; OPG: osteoprotegerin; RANK: receptor activator of nuclear factor-B; RANKL: receptor activator of nuclear factorB ... ratio of 1:100 and (C) a cell-particle ratio of 1:500 Time course of UHMWPE particle-induced OPG mRNA expression after treatment with different alpha-CGRP doses in (E) a cell-particle ratio of 1:100...
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