Hydrogen peroxide as a potential biomarker of oxidative stress is there a reliable assay

Tài liệu Báo cáo khoa học: Enhancement of oxidative stress-induced apoptosis by Hsp105a in mouse embryonal F9 cells pptx

... Hsp105 enhances oxidative stress-induced apoptosis (Eur J Biochem 269) 4149 Fig Suppression of oxidative stress-induced apoptosis in F9 cells by a potent p38 inhibitor F9, V1, S3 and S23 cells were ... activation of p38 induced by oxidative stress, we further examined whether its activation is responsible for the induction of apoptosis in F9 cells using SB202190, a potent inhibitor of p38 As shown in ... enhanced in both S3 and S23 cells compared with F9 and V1 cells (Fig 6B) Thus, over-expression of Hsp105a seemed to enhance the oxidative stress-induced activation of p38 but not JNK in F9 cells As Hsp105a...

The effects of oxidative stress on female reproduction: a review ppt

... antioxidant supplementation as a possible management approach for these patients 10 Pregnancy complications 10.1 The placenta The placenta is a vital organ of pregnancy that serves as a maternal-fetal ... indicating DNA damage by OS However, Tamura et al (2008) found that the administration of melatonin led to a reduction of intrafollicular oxidative damage and a net increase of fertilization and ... Harada T, Taniguchi F, Izawa M, Ohama Y, Takenaka Y, Tagashira Y, Ikeda A, Watanabe A, Iwabe T, Terakawa N: Apoptosis and endometriosis Front Biosci 2007, 12:3140–3151 111 Yamaguchi K, Mandai...

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Báo cáo khoa học: Oxidative stress is involved in the permeabilization of the inner membrane of brain mitochondria exposed to hypoxia/reoxygenation and low micromolar Ca2+ Lorenz Schild1 and Georg Reiser2 doc

... that mitochondria contribute to the induction of oxidative stress during ischemia ⁄ reperfusion in brain There is a growing body of information concerning the mechanism of ROS generation by the ... ADP inhibits opening of the mPTP by occupying binding sites located in the inner and outer mitochondrial membrane [39,40] The binding of ADP stabilizes the matrix conformation of the adenine ... brain mitochondria in the presence of these electron donors This is of relevance in brain, because in this tissue glucose is metabolized providing the NADH-yielding substrate pyruvate Under these...

Báo cáo khoa học: Effect of oxidative stress and involvement of poly(ADP-ribose) polymerase (PARP) in Dictyostelium discoideum development doc

... fruits PARP involvement during oxidative stress- induced developmental changes in D discoideum To determine the role of PARP in oxidative stressinduced developmental changes, D discoideum cells were ... Rajawat et al PARP in Dictyostelium discoideum development 20 microns Fig Effect of PARP inhibition during oxidative stress- induced developmental changes in D discoideum Cells were preincubated with ... the role of PARP in D discoideum development by inhibiting its activity with the known PARP inhibitor benzamide, and studied its effects on development and oxidative stressinduced development Our...

Commons-based Peer-Production of Physical Goods Is there Room for a Hybrid Innovation Ecology? pdf

... Commons-based Peer-Production of Physical Goods Is there Room for a Hybrid Innovation Ecology? Most of these statements came from existing labs; managers of planned labs referred to talking about ... these statements, however, came from people who don’t actually have a Fab Lab yet; and sharing and availability of information is also mentioned as a pain (see below) Innovation was another big ... people what Fab Labs are—you know—people that don't know; or that we can actually use the information in a constructive way.’ An issue that only managers and assistants of existing Fab Labs mentioned...

Báo cáo khoa học: Cyclosporin A-induced oxidative stress is not the consequence of an increase in mitochondrial membrane potential doc

... control substance in this context, because it is an inhibitor of P-glycoprotein rather than MPTP, and is devoid of calcineurin-inhibiting properties [23] We hypothesized that an increase in steady-state ... Cyclosporin A-induced oxidative stress M van der Toorn et al calcineurin therefore play a role in cyclosporin A-induced oxidative stress and disturbed Ca2+ homeostasis Immunosuppressive ... NIM811 and PSC833 induce an increase in cytosolic [Ca2+] and production of ROS The discrepancy in the effects of PSC833 between GLC4 cells and isolated mitochondria versus the consistency in the...

báo cáo hóa học: " The microglial NADPH oxidase complex as a source of oxidative stress in Alzheimer''''s disease" ppt

... interaction of A with microglia and the assembly of the active microglial NADPH oxidase maybe largely responsible for the oxidative damage observed in the AD brain Astrocytes and the NADPH oxidase Astrocytes ... complex and the initiation of intracellular signaling events regulating oxidase assembly and activation has been described [31,32] The NADPH oxidase The phagocytic NADPH oxidase plays an essential ... linkage between these oxidative events and the microglial NADPH oxidase has not been established Antioxidant therapy and the NADPH oxidase Considerable attention has been devoted to antioxidants...

báo cáo hóa học: " Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers" pptx

... article as: Sauvain et al.: Biomarkers of oxidative stress and its association with the urinary reducing capacity in bus maintenance workers Journal of Occupational Medicine and Toxicology 2011 ... verified by testing the correlation between levels of 8OHdG, reflecting oxidative stress, and the reducing capacity (corresponding to a defense against oxidative stress) in the urine of the particle-exposed ... oxidative stress The observed increase of reducing species in urine would mirror an increased level in blood originating from a response to oxidative stress in the body monitored by the urinary 8OHdG...

Báo cáo khoa học: " Induction of oxidative stress and lipid peroxidation in rats chronically exposed to cypermethrin through dermal application" pps

... 258 Rajinder Raina et al Table Effects of chronic dermal application of cypermethrin on enzymes, blood glutathione and lipid peroxidation in Wistar rats Days after dermal application ... 181-193 Taplin D, Meinking TL Pyrethrins and pyrethroids in dermatology Arch Dermatol 1990, 126, 213-221 Trojanowski JQ Rotenone neurotoxicity: a new window on environmental causes of Parkinson’s ... be attacking the thiol group of cysteine residuse and polyunsaturated fatty acids of biological membranes [6] Free radical-induced lipid peroxidation resulting in the deterioration of biological...

Báo cáo y học: "Proteinase-3 as the major autoantigen of c-ANCA is strongly expressed in lung tissue of patients with Wegener’s granulomatosis" pptx

... and distribution of PR-3 in the normal lung and in the lung tissue of patients with WG To contribute to this little-known issue, we examined the PR-3 expression in normal lung and lung tissue of ... that PR-3 mRNA is expressed in normal lung tissue, we questioned whether the expression pattern is different in the lung tissue of WG patients The examined specimens of patients with WG showed ... mostly expressed in pneumocytes type I and type II PR-3 expression was pronounced at sites of inflammatory infiltration, vasculitis and granulomas In comparison with normal lung tissue, however, there...

Báo cáo y học: "Potential involvement of oxidative stress in cartilage senescence and development of osteoarthritis: oxidative stress induces chondrocyte telomere instability and downregulation of chondrocyte function" pptx

... oxidative stress in OA cartilage, as discussed above, we postulated that oxidative stress induces telomere instability and dysfunction in chondrocytes, subsequently resulting in cartilage ageing and ... pathogenesis of OA from the viewpoint of oxidative stress induced genomic instability, especially telomere erosion, and chondrocyte senescence Our findings clearly show the presence of oxidative stress in ... increases the telomere shortening rate by up to one order of magnitude [46] From these findings, we postulated that oxidative stress directly induces chondrocyte telomere instability in OA cartilage...

Báo cáo khoa học: "Oxidative stress is increased in critically ill patients according to antioxidant vitamins intake, independent of severity: a cohort study" pptx

... with intake of antioxidant vitamins ...

Báo cáo y học: "Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats" potx

... effects of the hydrogen sulfide donor Moreover, we did not assess the effects of NaHS on inflammation and oxidative stress in non hemorrhagic rats since the injection of a single dose of 0.2 mg/kg of ... et al.: Effects of hydrogen sulfide on hemodynamics, inflammatory response and oxidative stress during resuscitated hemorrhagic shock in rats Critical Care 2010 14:R165 Submit your next manuscript ... hydrogen sulfide in a murine model of acute lung injury induced by combined burn and smoke inhalation Clin Sci (Lond) 2008, 115:91-97 31 Maines MD, Mayer RD, Ewing JF, McCoubrey WK Jr: Induction of...

Gamma glutamyltransferase is it a biomarker of oxidative stress

... form: Gamma- glutamyl-X + acceptor  Gamma- glutamyl-acceptor + X A wide range of compounds can be used as a gamma- glutamyl donor or as acceptor The most natural substrate is GSH (gamma- glutamyl ... MTS assay manual) The absorbance of formazan was measured at 490nm This assay measures the dehydrogenase enzyme activity found in metabolically active cells and procedures of this assay were carried ... translation regulation by the 5’ untranslated region of a GGT mRNA, was initially found using HepG2 cells, and appears to serve as a tissue- specific translational enhancer82 Gamma- glutamyltransferase...

Hydrogen peroxide as a potential biomarker of oxidative stress is there a reliable assay

... commonly-used biomarkers of oxidative stress/ damage and the diseases with which they are associated 13 Table 1.2 Biomarkers of oxidative stress/ damage associated with some human diseases (adapted from Valko ... biomarkers of oxidative stress/ damage isolated from tissues and biological fluids Biomarkers are defined as characteristics that can be objectively measured and evaluated as indicators of normal biological ... analyzed by DCFH assay 100 3.24 Effect of dilution of urine sample on DHR assay and comparison with DCFH assay 106 3.25 DHR assay recovery study and comparison with DCFH assay 107 3.26 Variations in...

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