... values for the 20 lowest-energy conformers of the best cluster are ˚ ˚ 0. 71 ± 0 .11 A for the backbone and 1. 11 ± 0.09 A for the heavy atoms The mean total energy of the ensemble is ) 814 6 kcalÆmol )1, ... saltbridge interactions are found between the carboxylate group of E422 and the amine groups of K43 and K44 of monomer B, and the side chains of R4 21 and D12 of monomer A These residues point towards the ... his3-200, ade2 -10 1, lys2-8 01, trp1-9 01, leu2-3, 11 2, gal4D, gal80D, cyhr2, LYS2::GAL1UAS-HIS3TATA-HIS3,MEL1; URA3::GAL1UAS-GAL1TATA-lacz) was used for all yeast two-hybrid assays The pre-transformed...