... ofthe first 88amino acids of PPI1 may participate in the interaction with the H+-ATPase and makes PPI1588His6a suitabletool to study the mechanism of action of PPI1. The analysis ofthe ... coli strain XL10 and the frame andthe identity ofthe cloned fragment verified bysequencing. The construct with the N-terminal portion of PPI1 has been previously described [22]. The DNA fragment ... H+-ATPase in the PM and stimulate itsactivity [22], suggested that the site ofinteraction with the PM H+-ATPase was localized in the N-terminus of PPI1. To further characterize the biological...
... at the time of chloroplast origin had poorly definedinteractions between Cyt f and plastocyanin and/ or plasto-cyanin and photosystem I, and subsequently the nature of the interactions in the ... Hencefor both organisms, the changes in DSà of the electronicfactor would have the same sign as the measured trend.Further discussion oftheeffectsof ionic strength onthermodynamic parameters ... Hence, the dependence of k2 of wild-type and all mutants on the concentration of MgCl2was investigated at 278, 283, 288, 293 and 298 K.Table 1. Kinetic and thermodynamic parameters ofthe reaction...
... of ISP and the extramembranous cd2 helix of cytochrome b areimportant for maintaining the structure ofthe hinge region and, by consequence, the movement ofthe headgroup and the integrity of ... region andthe cytochrome bextramembranous cd2 helix is important for maintaining the structure ofthe hinge region and, by consequence, the movement ofthe headgroup andthe integrity ofthe enzyme.Keywords: ... below.Instability ofthe mutant enzymeFurther analysis ofthe kinetic parameters ofthe G167Emutant was hindered by rapid inhibition ofthe activity of the bc1complex during the assay, which...
... particular, the key interactionbetween the ligand andthe receptor was determined to be the ionic interaction involving the ligand’s TyrNH and Asp147 of TMH III, as reported for several otheropioid ... A˚ of com-pound 3, one between Oc1 of Thr218 (EL-2) and the backbone oxygen of Phe4 (2.94 A˚), and one between the hydroxyl oxygen ofthe Tyr1 group and OAla240(TMH V) of 2.66 A˚. The ... Cys and His) located on the surface ofthe bind-ing site, and this polarization effect can be obtained byusing the QM description ofthe ligand. Looking at the electronic structure ofthe ligands...
... andthe humidity of the paper itself. Humidity balance, then, is the relation between the humidity of a material andthe humidity ofthe surroundingair. As long as both values are balanced, the ... blistering, such as the type of binding agents and coating pigments used, the amount of binding agents contained in the paper andthe amount of coating used, andthe degree to which the surface has ... figure to the right). The main sources of breaking in the fold are the extremetemperatures the paper is subjected to in the drying oven and the pressure applied in the folding unit. The single...
... at the tip of a loop protruding away from the core of the protein. This amino-acid motif mimics RGD in terms of charge and is positioned at the tip of a loop and istherefore reminiscent of RGD ... illustratedby the superimposition ofthe 3D structures of kistrin[32] and cardosin A (Fig. 8). The importance of bothmotifs and in particular their basic residues was fur-ther emphasized by the complete ... by the complete lack of interaction betweenthe C2 domain andthe double mutated(AGD ⁄ AGE) cardosin A. The docking model shownin Fig. 9 further highlights the role of RGD and KGEin complex...
... Scotland to England, ofthe long resistance ofthe Celts of Scotland to the English of Scotland".[4] Above all, the conception has been firmly planted in the imagination by the poet of the ... schiltrons of pikemen, underRandolph, Edward Bruce, and Sir James Douglas, and Bruce himself commanded the reserve, which wascomposed of Highlanders from Argyll andthe Islands andofthe men of Carrick.[49] ... Bruce of Annandale, who had been promised the succession by Alexander II, and had been disappointed ofthe fulfilment of his hopes by the birth ofthe lateking in 1241. The deaths of two of the...
... slow release ofthe cleavage products from the protein. The amplitudes ofthe initial burst (2.78 and 2.83 nM) gave a ratio of DNA/protein of 0.94 : 1. As will bediscussed further, these data ... hypotheses will needto be performed to determine the binding characteristics of the nucleic acid substrates, both alone and in the presence of one another.Although the structural details of ... about 45 min. The substitution of either Na+for K+or acetate for Clions, as well as the inclusion of 0.1 mgặmL)1BSA did notincrease the stability ofthe protein and slowed the reactionrate...
... in most ofthe houses andthe maximum ratio between the number of people living in a house and its number of rooms is 2:1. Therefore the extended family is the main network of support and information ... healthy lifestyle. Another criterion for the evaluation ofthe intervention, and, simultaneously, ofthe success ofthe project, is the level of community participation in the social information ... lack of education andof health information programs. The lower interest in health among the elderly in Romania reflects the problems ofthe national health system. Household structure and the...
... transferred from the N lone pair into the s* anti-bonding orbital ofthe SH bond. This transfer is facili-tated by the overlap ofthe N lone pair with the lobe of the s* orbital proximate to the S atom, ... Weinheimwww.chemphyschem.org3537 Interaction between an Amide and S-Containing Molecules a number of different noncovalent interactions, but the E(2)values of all of them are only around 0.52 kcal molÀ1. The comparison of ... 1b and 1c indicates that the benefit of formingCH···S and SH···p H-bonds, even weak ones, is worth the stretching and bending ofthe SH···O in 1b. The next minimum on the surface,...
... the difference in the circulating concentrations of Gamide and trans-ferrin. In the present study, we explored further the interactionbetween Gamide and transferrin, and characterized theinteraction ... ApoTfinto the BIAcore channel to determine whether the fer-ric ions were required for theinteractionbetween gast-rins and ApoTf. In the presence of EDTA, no interaction between ApoTf and either ... IC50 of 96 ± 1 lM and an inter-cept of 115%. (D) Theinteractionbetween Ggly and ApoTf thatonly binds iron in the C-lobe (Mono C). The line of best fit wasdrawn with an IC50 of 64 ± 1 lM and...
... respectively, and [EL] is the concentration of boundligand. The total concentration of sites can be expressed asN[E]0, the product of total protein concentration ([E]0) and the number of binding ... contact in the absence of ATP, curcumin is then able tobind to the ATPase (possibly at the hinge region) locking the two domains together and therefore precluding ATP binding(i.e. inhibiting the ATPase ... order to further assess the possibility of curcuminaffecting theinteractionof ATP binding to the ATPase, thiswas directly measured using [32P]ATP in the absence of Ca2ỵ(Fig. 4A). The data...
... detection of androgen receptorN/C interaction The ligand-dependent interactionbetween AR NTD and AR LBD, N/C interaction, was studied in yeast and mammalian in vivo protein interaction systems, and ... Netherlands The N-terminal domain (NTD) andthe ligand-bindingdomain (LBD) ofthe androgen receptor (AR) exhibit aligand–dependent interaction (N/C interaction) . Aminoacids 3–36 in the NTD (AR3)36) ... 2002 Interactionbetween androgen receptor subdomains (Eur. J. Biochem. 269) 5787 Amino acids 3–13 and amino acids in and flanking the 23FxxLF27motif modulate theinteractionbetweenthe N-terminal...
... D(316–344) and D(495–535) for the interactionbetweenthe N-terminal SBDs and CD.Our data also show that the full integrity of the starch-binding capacity, particularly ofthe D2 domain,modulates the ... capacity and also modulating the catalytic properties ofthe enzyme [19]. To extend the information about the role ofthe noncatalyticSBD regions and their effect on the C-terminal CD,we further ... areinvolved in theinteraction with CD, and that this interaction enhances the catalytic activity of the enzyme. Our results show that theinteraction between SBDs and CD, as well as the full starch-binding...