Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF ... Because of the essential roles of Oct4 and Stat3 in maintenance of stem cell pluripotency in mammals, and the unique features of the medaka, it seems equally important to understand the roles of Oct4 ... by Oct4 and Sox2 97 3.1.9.2.3 OSE alone is sufficient for regulated expression 3.2 Cloning and characterization of two isoforms of the medaka stat3 104 109 3.2.1 Isolation of stat3 cDNAs from the...

Báo cáo Y học: Structural and biochemical characterization of calhepatin, an S100-like calcium-binding protein from the liver of lungﬁsh (Lepidosiren paradoxa) docx

... analysis The 105 000 g supernatant of lungﬁsh liver (lane 1), skeletal muscle (lane 2), intestine (lane 3), lung (lane 4), brain (lane 5), adipose tissue (lane 6), heart (lane 7) and skin (lane ... showed that the protein is in a monomer–dimer equilibrium and that the dissociation constant is in the micromolar range for the apoprotein and in the submicromolar range for the holoprotein, as ... structures of other members of the family The molecule is depicted in strand representation, and a helices are numbered from I to IV (B) Residues Leu7, Arg8 and Phe11 from a-helix I, and Trp60,...

Báo cáo y học: "nalysis of the mouse embryonic stem cell regulatory networks obtained by ChIP-chip and ChIP-PET" pptx

... range of threshold values Embryonic stem (ES) cells are derived from the inner cell mass of the embryo and possess the property of pluripotency, which is the ability to develop into any cell lineage ... set of OCT4- and NANOG-regulated genes, by collectively examining the targets identified by ChIP-chip and ChIP-PET The functional relevance of the ChIP-chip and ChIP-PET data, as examined by GO ... for the chromosome array These represent the fraction of ChIP-PET recovery under a range of ChIP-chip p-value cut-offs (b, d) OCT4 (b) and NANOG (d) ChIP-chip recovery curves These show the ChIP-chip...

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Puriﬁcation of four pulchellin isoforms from A pulchellus seeds Using a combination of afﬁnity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5 .2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...

Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys docx

... Cite this article as: Abdel-Moneim et al., Isolation and characterization of highly pathogenic avian influenza virus subtype H5N1 from donkeys Journal of Biomedical Science 2010, 17:25 ... Fatal avian influenza A H5N1 in a dog Emerg Infect Dis 2006, 12:1744-1747 11 Li HY, Yu K, Yang H, Xin X, Chen J, Zhao P, Bi Y: Isolation and characterization of H5N1 and H9N2 influenza viruses from ... Experimental infection of cattle with highly pathogenic avian influenza virus (H5N1) Emerg Infect Dis 2008, 14:1132-1134 17 Cardona CJ, Xing Z, Sandrock CE, Davis CE: Avian influenza in birds and mammals...

báo cáo khoa học: "Public perception of stem cell and genomics research" ppt

... principles for responsible translational stem cell research Cell Stem Cell 2008, 3:607-609 ISSCR: A closer look at stem cell treatments [http://www closerlookatstemcells.org//AM/Template.cfm?Section=Home] ... line is this: combining stem cell and genome technologies is a terrific idea I foresee a deeper understanding of human development and disease as a result of this union and, thus, a shorter path ... hESC, human embryonic stem cell; HGP, Human Genome Project; hPSC, human pluripotent stem cell; iPSC, induced pluripotent stem cell; ISSCR, International Society for Stem Cell Research Competing...

báo cáo khoa học: " Characterization of WRKY co-regulatory networks in rice and Arabidopsis" ppsx

... AtWRKY33 AtWRKY18 AtWRKY40 AtWRKY22 AtWRKY46 or AtWRKY53 AtWRKY75 AtWRKY46 or AtWRKY53 AtWRKY69 AtWRKY6 or AtWRKY31 AtWRKY46 AtWRKY54 or AtWRKY70 AtWRKY54 or AtWRKY70 AtWRKY26 or AtWRKY2 AtWRKY34 ... OsWRKY4 OsWRKY18 OsWRKY43 OsWRKY61 OsWRKY71 OsWRKY87 OsWRKY100 OsWRKY112 M oryzae BR32 OsWRKY4 OsWRKY18 OsWRKY40 OsWRKY43 OsWRKY61 OsWRKY64 OsWRKY87 OsWRKY100 OsWRKY112 M oryzae FR13 OsWRKY4 OsWRKY53 ... single zinc finger motif of a small subset of WRKY proteins is distinct from that of group and members Instead of a C2H2 pattern, their WRKY domains contain a C2HC motif As a result of this distinction,...

Báo cáo khoa học: "Isolation and characterization of a genotype 4 Hepatitis E virus strain from an infant in China" ppt

... Bezanilla JC, Toth TE, Meng XJ: Identification of genotype hepatitis E virus (HEV) in serum and faecal samples from pigs in Thailand and Mexico, where genotype and HEV strains are prevalent in ... genotype HEV strains with complete genome available in GenBank (including both swine and human original isolates) were used as references in the analysis; their GenBank accession numbers and source ... aimed to determined the whether this genotype HEV strain is prevalent in Shanghai swine or not HEV paly an important role in acute sporadic and epidemic viral hepatitis in Asia and Africa Although...

Báo cáo y học: "Regulatory conservation of protein coding and microRNA genes in vertebrates: lessons from the opossum genome" pptx

... the importance of including the opossum genome in the comparisons The grey line displays the same graph, but excluding the opossum genome from the plotted combinations Without including the opossum ... similar to coding genes The following question then arises; how does the conservation in the upstream regions of the intergenic miRNA genes compare with that of the protein coding genes? In this ... conservation of the miRNA genes is almost identical to that of the promoters of the protein coding genes (R values > 0.9 and usually much higher; regression t-test: P < 10-19) In protein coding genes...

Báo cáo y học: "Histone deacetylase inhibition accelerates the early events of stem cell differentiation: transcriptomic and epigenetic analysis" doc

... and Lefty2 Among the early and strongly TSAsupressed genes are Sall1, Gli2 and Klf2, which have not been previously associated with regulation of pluripotency and may be novel candidates In the ... HY, Soh BS, Lou Y, Yang J, Ma Y, Chai L, Ng HH, Lufkin T, Robson P, Lim B: Sall4 modulates embryonic stem cell pluripotency and early embryonic development by the transcriptional regulation of ... in wildtype cells whereas Brachyury was up-regulated one day later than in wild-type cells These results show that 'ES re' cells are still capable of acquiring either one of the three cell fates...

Báo cáo y học: "Developmental stage related patterns of codon usage and genomic GC content: searching for evolutionary fingerprints with models of stem cell differentiation" potx

... mechanisms for these developmental stage- related patterns of codon usage This study reveals that developmental stage- related patterns of gene expression are correlated with GC3 and GCg in models of stem ... 8:688-693 Kanaya S, Yamada Y, Kinouchi M, Kudo Y, Ikemura T: Codon usage and tRNA genes in eukaryotes: correlation of codon usage diversity with translation efficiency and with CG-dinucleotide usage ... of developmental stage- correlated codon usage and GCg Materials and methods Conclusion In this investigation, using models of stem cell differentiation, developmental stage- related patterns of...

Characterization of MP2 cell division and pins function on spindle asymmetry of drosophila central nervous system

... CHARACTERIZATION OF MP2 CELL DIVISION AND PINS FUNCTION ON SPINDLE ASYMMETRY OF DROSOPHILA CENTRAL NERVOUS SYSTEM LIN SHUPING (M.Sc.) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF PHILOSOPHY ... is Pins independent 91 3.2.3 MP2 asymmetric cell division is Baz dependent 92 3.3 Discussion 95 CHAPTER Characterization of Pins function and G protein signaling on spindle asymmetry during Drosophila ... asymmetric division come from the invertebrate model systems, Drosophila and C elegans The first division of C elegans one -cell embryos and the embryonic neuroblast (NB) divisions of Drosophila...

Identification and characterization of iron homeostasis related genes and HCC down regulated mitochondrial carrier protein (HDMCP), a novel liver specific uncoupling protein in human hepatocellular carcinoma (HCC

... IDENTIFICATION AND CHARACTERIZATION OF IRON HOMEOSTASIS- RELATED GENES AND HCCDOWN -REGULATED MITOCHONDRIAL CARRIER PROTEIN (HDMCP), A NOVEL LIVER- SPECIFIC UNCOUPLING PROTEIN IN HUMAN HEPATOCELLULAR ... 5’-RACE β2m mitochondrial membrane potential 5'-rapid amplification of cDNA ends β2-microglobulin aa AAH AFP ALAS ANOVA ATP amino acid atypical adenomatous hyperplasia α-fetoprotein δ-aminolevulinate ... value of protein induced by vitamin K absence (PIVKAII) and hepatoma -specific band of serum gamma-glutamyl transferase (GGTII) as hepatocellular carcinoma markers complementary to alphafetoprotein...

Báo cáo y học: "Characterization of erythrovirus B19 genomes isolated in liver tissues from patients with fulminant hepatitis and biliary atresia who underwent liver transplantation"

... prevalence of B19 infection in livers from patients with fulminant hepatitis and biliary atresia Interestingly, all fulminant hepatitis patients infected with B19 had the replicative form of B19 mRNA ... prevalence of B19 infection not only in fulminant hepatitis patients, but also in biliary atresia patients However, all fulminant hepatitis patients infected with B19 had the replicative form of B19 ... for hepatitis E or parvovirus B19 infection in patients with acute liver failure To investigate the possibility that B19 infection of the liver might cause fulminant hepatitis and biliary atresia, ...

Tài liệu Báo cáo khoa học: Puriﬁcation and structural characterization of a D-amino acid-containing conopeptide, conomarphin, from Conus marmoreus docx

... Kumagaye KY, Nakajima K, Watanabe T, Kawai T, Kawakami Y, Niidome T, Sawada K, Nishizawa Y et al (1994) Omega-agatoxinTK containing D-serine at position 46, but not synthetic omega-[L-Ser46]agatoxin-TK, ... Inoue A, Kawakami Y, Nishizawa Y, Katayama K & Kuwada M (1995) Isolation and characterization of a peptide isomerase from funnel web spider venom J Biol Chem 270, 16719–16723 Torres AM, Tsampazi ... assigned and integrated, with concomitant cycles of structure calculations for evaluation of distance and angle constraint violations as well as assignments of additional peaks based on the preliminary...

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Từ khóa: identification of stem cell specific genes isolation and culture from the neonatal olfactory bulb it s all in the genes the ethics of stem cell and genetic research role of stem cell imaging in regenerative medicine gabriella passacquale and kishore bhakoo relapsed refractory disease and the role of stem cell transplant number time and method of stem cell delivery the obstacles on the road to clinical applications of stem cell based therapies what has been done to overcome these obstacles and what remains to be done ethical aspects of stem cell based clinical translation research innovation and delivering unproven intervention banks repositories and registries of stem cell lines the challenges to legal regulation ethical legal and social implications of translational stem cell research effects of commercialization on public opinion and trust of stem cell research pattern morphology and expression of stem cell markers are affected by the culturing medium identification of stem cell protection mechanism related genes 29 soosan g lorne bt 2005 organization of stem cell and their progeny in human epidermis journal of invest dermatol vol 124 367 372 generation and characterization of isogenic cell lines expressing p53pro and p53arg generation and characterization of isogenic cell lines expressing p53pro or p53arg chuyên đề điện xoay chiều theo dạng Nghiên cứu sự hình thành lớp bảo vệ và khả năng chống ăn mòn của thép bền thời tiết trong điều kiện khí hậu nhiệt đới việt nam Một số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTP Nghiên cứu vật liệu biến hóa (metamaterials) hấp thụ sóng điện tử ở vùng tần số THz đề thi thử THPTQG 2019 toán THPT chuyên thái bình lần 2 có lời giải Biện pháp quản lý hoạt động dạy hát xoan trong trường trung học cơ sở huyện lâm thao, phú thọ Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Giáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôit Phối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọ Phát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninh Nghiên cứu về mô hình thống kê học sâu và ứng dụng trong nhận dạng chữ viết tay hạn chế Sở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIX Kiểm sát việc giải quyết tố giác, tin báo về tội phạm và kiến nghị khởi tố theo pháp luật tố tụng hình sự Việt Nam từ thực tiễn tỉnh Bình Định (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 15: Tiêu hóa ở động vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật Đổi mới quản lý tài chính trong hoạt động khoa học xã hội trường hợp viện hàn lâm khoa học xã hội việt nam MÔN TRUYỀN THÔNG MARKETING TÍCH HỢP TÁI CHẾ NHỰA VÀ QUẢN LÝ CHẤT THẢI Ở HOA KỲ QUẢN LÝ VÀ TÁI CHẾ NHỰA Ở HOA KỲ