banks repositories and registries of stem cell lines the challenges to legal regulation

Tài liệu INNOVATIONS IN STEM CELL TRANSPLANTATION doc

Ngày tải lên : 18/02/2014, 04:20

... between the presence of KIR genes and their HLA li‐ gands and cytotoxicity and 2) the advent of methods of precise genetic characterization, it is possible to determine the contributions of the various ... progenitor cell is similar to a stem cell, although they are limited in the number of times they can replicate and they are also restricted in which cells they can fur‐ ther differentiate to Serving ... readers Stem cells have a unique ability; they are able to self renew limitlessly allowing them to re‐ plenish themselves as well as other cells Another ability of stem cells is that they are able to...

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Tài liệu New Advances in Stem Cell Transplantation Edited by Taner Demirer ppt

Ngày tải lên : 20/02/2014, 08:20

... allowing them to replenish themselves, as well as other cells Another ability of stem cells is that they are able to differentiate to any cell type A stem cell does not differentiate directly to a ... order to replenish other cells for the rest of the person or animal's natural life When a stem cell divides, each new cell has the potential to either remain a stem cell, or become another type of ... limited in the number of times they can replicate, and they are also restricted in which cells they can further differentiate to Serving as a sort of repair system for the body, they can theoretically...

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Báo cáo khoa học: Transient potential receptor channel 4 controls thrombospondin-1 secretion and angiogenesis in renal cell carcinoma ppt

Ngày tải lên : 07/03/2014, 05:20

... temperature and added to the cells After 24, 48 and 72 h, CM were collected and the cells were processed further (total RNA and ⁄ or cell lysates) Cell survival ⁄ proliferation assay The cells were ... levels in the cytoplasm and CM of P769 cells, and the lack of response to BAPTA-AM C, puriﬁed TSP1 or VEGF, respectively A B C D Fig Calcium channels and calbindin in HNK and P769 cells (A, B) ... endothelial cell apoptosis, which requires receptors CD36 and Fas, and Fas ligand [38], and causes CD36-independent cell cycle arrest [39] TSP1 suppresses recruitment of the circulating endothelial...

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Báo cáo khoa học: Superoxide radical-scavenging effects from polymorphonuclear leukocytes and toxicity in human cell lines of newly synthesized organic selenium compounds potx

Ngày tải lên : 23/03/2014, 07:20

... 0.05 Cytotoxicity of organic selenium compounds in human cells We examined the cytotoxic effects of selenoureas and selenoamides using three cell lines, HaCaT, HEL, and HEp-2, and PMNs, by the microtiter ... microscopy (LSM) The cytosolic component of NADPH oxidase complex, p47phox, translocates from the cytosol to the cell membrane upon activation To examine the effects of selenoureas and selenoamides ... system catalyzes the production of O2– A component of NADPH oxidase, p47phox, translocates to the cell membrane and associates with cytochrome b556 to form the active complex that catalyzes the...

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Báo cáo sinh học: "Combination therapy with vemurafenib (PLX4032/RG7204) and metformin in melanoma cell lines with distinct driver mutations" pdf

Ngày tải lên : 18/06/2014, 19:20

... for the care of melanoma cell lines and cell viability studies and from BC-A for the optimization and conduct of cell viability, cell cycle, apoptosis and phosphoflow studies: JAG, JAR, RSL and ... analysis of the BRAFV600E mutant cell lines M263 (top) and SKMEL28 (bottom) in low glucose media For these studies, the BRAFV600E mutant cell lines M263, resistant to vemurafenib and sensitive to metformin, ... agent therapy To further characterize the effects of the combination, 10 cell lines were chosen for further detailed analyses of cell signaling and proliferation (Table 2) They represented the...

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The role of paxillin superfamily members hic 5 and leupaxin in b cell antigen receptor signaling 1

Ngày tải lên : 12/09/2015, 08:16

... regulation of antigen receptor signaling in lymphocytes (Yamasaki and Saito, 2004) Most of these inhibitory adaptors function by recruiting inhibitory effectors to the vicinity of positive regulators ... On the other hand, DAG activation leads to activation of other PKC isotypes The classical PKC isoforms ( , I, II and γ) are activated by both Ca2+ and DAG; the novel PKC isoforms (δ, ε, θ and ... longer binds Csk and hence releases Csk to cytosol removing the inhibition of Lyn (Brdicka et al., 2000) This leads to the autophosphorylation of the Y416 residue in Lyn and thereby triggering...

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The role of paxillin superfamily members hic 5 and leupaxin in b cell antigen receptor signaling 2

Ngày tải lên : 12/09/2015, 08:16

... 4G10 to examine of LPXN were shown to be transthe phosphorylation of LPXN and with anti-FLAG and anti-HA antibodies to control for the expression of LPXN fected into A20 cells (Fig 6A) mutants and ... signaling pathway, e.g the adaptor protein BLNK and the tyrosine kinase Btk, are known to locate to the plasma membrane and especially to the lipid raft fraction of B cells following BCR activation ... downstream of the BCR signaling pathway Besides the tyrosine phosphorylation of LPXN, we also observed the recruitment of LPXN to the plasma membrane of B cells upon BCR ligation Several proteins in the...

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Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka

Ngày tải lên : 14/09/2015, 13:38

... of tissues; on the other hand, they have the potential to differentiate into various types of cells (Blau et al., 2001) It is the potential of stem cells Chapter I Introduction to give rise to ... develop into an entire new organism and every cell type Subsequently, these totipotent stem cells specialize into pluripotent cells that exist only in the inner cells mass (ICM) of the blastocyst ... status of self-renewing stem cells Stem cells show a hierarchy of developmental potency in mouse (Fig 1-1) Totipotent stem cells include the zygote and the 8 -cell morula that have full potential to...

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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt

Ngày tải lên : 20/06/2014, 07:20

... (MCF7) cell line [11] The fact that the vast majority of MCF7 stem cell enriched SP cells express MUC1 suggests that epithelial cancer stem cells would also be the targets of various immunotherapy ... for the isolation of cancer stem cells from different cancer tissues and cell lines [16,17] Qverexpressed MUC4 and control cells were stained with FITC-conjugated CD133 cells to analyze the percentage ... population of cells behaving like stem- cells are responsible for the tumor recurrence and disease aggressiveness [1] These populations of cells known as cancer stem cells have been demonstrated to have...

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báo cáo hóa học: " Function, Adjustment, Quality of Life and Symptoms (FAQS) in Allogeneic Hematopoietic Stem Cell " potx

Ngày tải lên : 20/06/2014, 15:20

... upon factors such as age, co-morbid illness, malignant disease status at the time of transplantation, intensity of the preparative regimen, source of stem cell graft, and the extent of histoincompatibility ... characteristics of our sample, and guided the development of hypotheses about the effects of these characteristics on functional status, the symptom experience, and HRQL in transplant survivors [65] The ... yearly surveys linked to the date of their enrollment Study Aims A revised version of the Wilson and Cleary [64] model of health-related quality of life is applied to describe the relationships among...

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Overexpression of hTERT increases stem-like properties and decreases spontaneous differentiation in human mesenchymal stem cell lines ppt

Ngày tải lên : 10/08/2014, 05:21

... co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure 3C) Previously, ... associated The gene expression profile of immortalized hMSCs is similar with that of ESCs To gain insight into the convergence of 3A6 toward ESCs, we compared the expression profile of 3A6 with ... http://www.appliedbiosystems.com) and the TaqMan Universal Master Mix (Applied Biosystems) Analysis of the results was carried out using the software supplied with the machine The software calculates...

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Báo cáo khoa học: " Challenging complications of treatment – human herpes virus 6 encephalitis and pneumonitis in a patient undergoing autologous stem cell transplantation for relapsed Hodgkin''''s disease: a case report" doc

Ngày tải lên : 12/08/2014, 04:21

... physicians in the department of hematology and oncology of the University of Ulm P.S is attending physician in the department of Diagnostic and Interventional Radiology of the University of Ulm Publish ... responsible for the patients care, PS interpreted the chest-CT and the MRI and added the figures, BM wrote the paper and all authors read and approved the final manuscript Authors' information G.J and B.M ... performed high-dose chemotherapy according to the BEAM protocol On day twelve after stem cell reinfusion he developed mental disturbance and convulsive status First MRI imaging of the brain showed no...

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Báo cáo khoa học: " Challenging complications of treatment – human herpes virus 6 encephalitis and pneumonitis in a patient undergoing autologous stem cell transplantation for relapsed Hodgkin''''s disease: a case report" ppsx

Ngày tải lên : 12/08/2014, 04:22

... physicians in the department of hematology and oncology of the University of Ulm P.S is attending physician in the department of Diagnostic and Interventional Radiology of the University of Ulm Publish ... responsible for the patients care, PS interpreted the chest-CT and the MRI and added the figures, BM wrote the paper and all authors read and approved the final manuscript Authors' information G.J and B.M ... performed high-dose chemotherapy according to the BEAM protocol On day twelve after stem cell reinfusion he developed mental disturbance and convulsive status First MRI imaging of the brain showed no...

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Exploring mesenchymal stem cell derived exosome and tocotrienol (t3) as therapeutic agents in drug induced liver injury (DILI)

Ngày tải lên : 09/09/2015, 11:21

... procaspase-9 and other pro-apoptotic mitochondria proteins [45] On the other hand, the extrinsic pathway involves the binding of death ligands expressed by Kupffer cells upon apoptosis to the respective ... Apoptosis and necrosis Apoptosis and necrosis are effector arms of liver injury, and therefore are good surrogate markers of the functional significance of any cytoprotective strategies Apoptosis ... intensity of the injury, ATP availability of the cell and the pre-existing susceptibility of hepatocytes [47, 48] In conclusion, mitochondria play a central role in determining the life and death of...

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Study of human nasal epithelial stem or progenitor cell growth and differentiation in an in vitro system

Ngày tải lên : 09/09/2015, 11:29

... inflammatory cells involve into the damaged site and play as the major roles in the development of NPs 2) Inflammatory cells and cytokines NP could be separated into two sub-types based on the pattern ... in NPs These results suggested that the impairment regulation of Treg and Th cells may contribute to the development of NP As the results of microorganism stimulation and inflammatory cells disorder, ... Additionally, the ectopic expression of epithelial cells cytokines (IL-25, IL-33 and TSLP) had the capacity to skew cell differentiation in Th2 direction (Schleimer et al., 2007) The change of these cytokines...

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Roles of long non coding RNAs in human embryonic stem cell pluripotency and neural differentiation 1

Ngày tải lên : 09/09/2015, 17:54

... The differentiation of pluripotent stem cells into neuroepithelial stem cells and radial glia Differentiation of hESCs or iPSCs into neuroepithelial cells requires the inhibition of the BMP and ... induction – lessons from the embryo One of the characteristics of pluripotent stem cells is the ability to differentiate into mature cell types of the three germ layers: ectoderm (including neural ... neural lineages), mesoderm and the endoderm However, the efficient differentiation of pluripotent stem cells into specific neural cell types requires exposure of the cells to the right culture conditions...