Towards automatic gene synthesis with bioinformatics software, novel one step real time PCR assembly, and lab chip gene synthesis 1
Towards automatic gene synthesis with bioinformatics software, novel one step real time PCR assembly, and lab chip gene synthesis 1
... Electrical and Computer Engineering Towards automatic gene synthesis with bioinformatics software, novel one- step real- time PCR assembly, and lab- chip gene synthesis Huang Mo Chao Under the supervision ... 51 Performance of TopDown one- step gene synthesis and its real- time analysis 51 4.4 .1 Performance of TD one- step gene synthesis 51 4.4.2 Analysis of real- time gene synthesis 54 4.5 ... 61 CHAPTER V AUTOMATIC TOUCHDOWN ONE- STEP GENE SYNTHESIS 63 5 .1 Introduction 63 5 .1. 1 5 .1. 2 5.2 Principle of Automatic TouchDown one- step gene synthesis 64 Mechanisms of PCR synthesis...
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Towards automatic gene synthesis with bioinformatics software, novel one step real time PCR assembly, and lab chip gene synthesis 2
... S100A4-1 7 52 66.8 9.1 3.0 30 19–33 19, 41–66 S100A4 -2 7 52 65 .2 2.03 0.48 32 18–39 18, 39–64 PKB2 1446 66 .2 1.9 0.59 62 16– 32 36–57 5 .2. 2 Automatic TouchDown one- step real- time gene synthesis Automatic ... TopDown one- step PCR gene synthesis and develop a more universal method, a novel approach – automatic TouchDown one- step PCR is developed 5.1.1 Principle of Automatic TouchDown one- step gene synthesis ... (ATD) one- step process was optimized using real- time PCR conducted with Roche’s LightCycler 1.5 real- time thermal cycling machine with a temperature transition of 20 °C/s Real- time gene synthesis...
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Tài liệu Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR pptx
... Comparison of lung cancer cell lines representing four histopathological subtypes with gene expression profiling using quantitative real-time PCR Cancer Cell International 2010 10:2 Publish with ... of 19 genes by qPCR The expression of 19 genes in test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared to a normal control cell ... and direct comparison of all four histopathological subtypes using the same method(s) was rare Our study is unique because we examined 12 lung cancer cell lines representing all four subtypes, ...
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Báo cáo y học: " Detection of porcine parvovirus using a taqman-based real-time pcr with primers and probe designed for the NS1 gene" doc
... Negative a, d indicate the number of samples that generated similar data for both assays b,d indicate the number of samples that generated different data for the assays The TaqMan real-time PCR assay ... and 72°C for 20 s The data were analyzed with the Rotor-Gene software Sensitivity of the real-time PCR To determine the detection limit and efficiency of the assay, recombinant plasmid of standard ... primers and probe were: NS1- FP (forward primer): 5’-GAAGACTGGATGATGACAGATCCA-3’, NS1- RP (reverse primer): 5’-TGCTGTTTTTGTTCT TGCTAGAGTAA-3’ NS1- P (probe) : FAM-AATGATGGCTCAAACCGGAGGAGA-BHQ1 The probe...
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Báo cáo khoa học: "Detection of anatid herpesvirus 1 gC gene by TaqMan™ fluorescent quantitative real-time PCR with specific primers and probe" pps
... 29.63 0. 21 0.70 1. 00E+04 33.07 0. 31 0.92 1. 00E+03 36.33 0. 31 0.84 1. 00E+02 39.50 0 .17 0.44 1. 00E+ 01 42.67 0.32 0.75 1. 00E+08 19 .70 0.28 1. 41 1.00E+07 1. 00E+06 23.09 26. 31 0.47 0.32 2.03 1. 23 1. 00E+05 ... ± 0 .19 8 .18 ± 0.08 3d 7.88 ± 0 .19 7.52 ± 0.07 7.78 ± 0 .11 7.5 ± 0.08 7.52 ± 0 .15 7. 91 ± 0.27 7. 41 ± 0 .19 7. 81 ± 0. 21 7.84 ± 0 .10 8 .12 ± 0.06 7.64 ± 0.09 8.02 ± 0.25 8.04 ± 0 .17 8 .11 ± 0 .17 7.82 ... 7.70 ± 0 .18 6.64 ± 0 .11 7. 61 ± 0 .19 7 .14 ± 0 .18 7.23 ± 0 .12 7.24 ± 0.07 7.76 ± 0 .14 7.39 ± 0 .15 7.84 ± 0.08 7 .11 ± 0 .15 7.30 ± 0 .16 6.84 ± 0.06 7.53 ± 0 .12 7.29 ± 0 .11 7.87 ± 0 .17 6.69 ± 0.05 7.35...
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Báo cáo sinh học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pdf
... Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with ... reliable housekeeping genes in cells infected with a variety of viruses [7,12], however to date no such study has been carried out in cells infected with human immunodeficiency virus (HIV) or the herpes...
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Báo cáo sinh học: " Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... ideal reference gene in gene expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes as reference ... synthesis was performed using µg of RNA, at 50°C Finally, cDNA was diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan probes and QPCR conditions for reference gene analysis were ... tested reference genes in a setting of virus infected human cell lines Capa- Page of (page number not for citation purposes) Virology Journal 2005, 2:7 ble reference genes were evaluated using three...
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Báo cáo hóa học: " Determination of suitable housekeeping genes for normalisation of quantitative real time PCR analysis of cells infected with human immunodeficiency virus and herpes viruses" pot
... Reference gene selection for quantitative real- time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections Virol ... of cell types infected with a variety of strains of HIV-1, HSV-1, VZV and cytomegalovirus (CMV) compared to mock infected cells Results A range of cell lines and primary cells were infected with ... shortlist of potential candidates To date the variability of housekeeping gene expression has been studied in cells infected with SARS corona virus, yellow fever virus, human herpes virus6 , camelpox virus, ...
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Báo cáo hóa học: " A broad spectrum, one-step reverse-transcription PCR amplification of the neuraminidase gene from multiple subtypes of influenza A virus" pdf
... samples from allantoic from all NA One-step RT -PCR amplification of NA gene from all NA subtypes using animal samples from allantoic fluids A fragment of approximately 253 bp was amplified using ... representation of 3,337 available sequences in the NCBI database at the time the study was conducted All NA subtypes were aligned against the NA10R primer and analyzed for discrepancies at the 3'end The ... [32-34] The percentage of samples with identical matches at all five 3' terminal bases was calculated for each NA subtype Figure NPA samples One-step RT -PCR amplification of NA gene from clinical One-step...
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báo cáo hóa học:" Reference gene selection for quantitative real-time PCR analysis in virus infected cells: SARS corona virus, Yellow fever virus, Human Herpesvirus-6, Camelpox virus and Cytomegalovirus infections" doc
... ideal reference gene in gene expression analysis has to be done for each individual experimental setting by evaluat- ing several genes and using the best two or three of these genes as reference ... synthesis was performed using µg of RNA, at 50°C Finally, cDNA was diluted 1:5 before use in QPCR Quantitative TaqMan PCR Primers, TaqMan probes and QPCR conditions for reference gene analysis were ... tested reference genes in a setting of virus infected human cell lines Capa- Page of (page number not for citation purposes) Virology Journal 2005, 2:7 ble reference genes were evaluated using three...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
... Similarly, synovial fluid can be obtained relatively easily by joint aspiration but its utility is markedly diminished by the fact that the volumes are highly variable and effusions are frequently ... measuring gene expression in target tissue The use of a cellular standard generated with activated PBMC cDNA significantly improves assay reliability by reducing variation and by simplifying assay development ... reproducible and accurate method of gene expression analysis on synovial biopsies, we evaluated and validated real-time quantitative PCR (Q-PCR) on very small synovial tissue samples using a novel...
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Phát hiện nhân tố gây bệnh (gene fima) của vi khuẩn p gingivalis bằng phương pháp real time PCR
... tài Phát nhân tố gây bệnh (gene fimA) vi khuẩn P. gingivalis phƣơng ph p real time PCR nhằm kiểm tra vi khuẩn phƣơng ph p ứng dụng thực tế 2 PHẦN TỔNG QUAN GIỚI THIỆU BỆNH VI M NHA CHU 1.1 Bệnh ... nhiên phƣơng ph p trƣớc vấn đề hạn chế tƣơng tự, phƣơng ph p realtime- PCR phƣơng ph p tối ƣu 18 PHẦN VẬT LIỆU VÀ PHƢƠNG PH P Vật liệu 1.1 Mẫu Khảo sát 30 mẫu dịch phết miệng bệnh nhân vi m nha ... 2.4 Phƣơng ph p real time PCR Trong nghiên cứu này, phƣơng ph p realtime PCR đƣợc thiết kế để phát Porphyromonas gingivalis (dựa trình tự đích gene 16S rRNA) tác nhân gây bệnh gene fimA Để phát...
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Tìm hiểu các kiểu đột biến liên quan đến sự kháng fluoroquinolone ở vi khuẩn lao và xác định nhanh vi khuẩn lao kháng fluoroquinolone bằng kỹ thuật real time pcr thông qua đột biến gene gyr
... vi khuẩn lao Vi t Nam Mục tiêu đề tài Tìm hiểu đột biến liên quan đến tính kháng fluoroquinolone vi khuẩn lao gene gyrA gyrB Phát triển quy trình Real- time PCR phát nhanh vi khuẩn lao kháng fluoroquinolone ... 1.1.2.4 Bệnh lao phổi Lao phổi tên gọi chung bệnh lao biểu quan khác phổi Các bệnh bao gồm: lao kê (bệnh lao lan tỏa, đa quan) , lao hạch, lao màng phổi, lao sinh dục – niệu, lao xương, lao hệ thần ... QRDR gene gyrA gene gyrB Phân tích kết giải trình tự so sánh với trình tự gene gyrA gene gyrB chủng H37Rv ngân hàng gene Hình 2.1 Sơ đồ thực vi c giải trình tự vùng QRDR gene gyrA gene gyrB chủng...
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Generation of mouse graves ophthalmopathy model with full length TSH receptor plasmid and cytokine evaluation by real time PCR
... tabulation of TSBAB status in the strains of mice immunized with TSHR Table 21 52 Cross tabulation of TSAB status in the strains of mice immunized with TSHR Table 20 51 61 Summary of cytokine profile and ... infiltration of muscular and connective tissues of the retroorbital space is a histological hallmark of Graves ophthalmopathy The pathogenesis of Graves ophthalmopathy and whether it is the result of ... models: 1) after genetic immunization of NMRI outbred mice treated with full length TSH receptor in an eukaryotic expression plasmid [32] and 2) after transfer of TSH receptor sensitized T cells in...
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engineering analysis with ansys software
... Engineering Analysis With ANSYS Software This page intentionally left blank Engineering Analysis With ANSYS Software Y Nakasone and S Yoshimoto Department of Mechanical Engineering ... copyrighted screen shots of the ANSYS software graphical interface that appear throughout this book are used with permission of ANSYS, Inc ANSYS and any and all ANSYS, Inc brand, product, service ... commercial products: ANSYS Mechanical or ANSYS Multiphysics, both general-purpose finite element analysis (FEA) computeraided engineering (CAE) software tools developed by ANSYS, Inc The company...
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Từ khóa: dealing with real time data streams and complex event processingisolation cdna synthesis and real time pcrcdna synthesis for real time pcrextraction cdna synthesis and real time pcrpkacβ and rgs4 gene expression by real time pcrsynthesis rt pcr and quantitative real time pcra room with a view novel pdftowards automatic data extraction from large websitesenglish to french dictionary with pronunciation software free downloadgone with the wind novel vs moviegone with the wind novel synopsisgone with the wind novel short summarylakes limnology and limnetic ecology towards a new synthesisdisabling automatic table creation with the simple membership provideri need high security with commercial software i can sue them if things go wrongBáo cáo thực tập tại nhà thuốc tại Thành phố Hồ Chí Minh năm 2018Nghiên cứu sự biến đổi một số cytokin ở bệnh nhân xơ cứng bì hệ thốngBáo cáo quy trình mua hàng CT CP Công Nghệ NPVMột số giải pháp nâng cao chất lượng streaming thích ứng video trên nền giao thức HTTPGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitGiáo án Sinh học 11 bài 13: Thực hành phát hiện diệp lục và carôtenôitĐỒ ÁN NGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWANNGHIÊN CỨU CÔNG NGHỆ KẾT NỐI VÔ TUYẾN CỰ LY XA, CÔNG SUẤT THẤP LPWAN SLIDEPhối hợp giữa phòng văn hóa và thông tin với phòng giáo dục và đào tạo trong việc tuyên truyền, giáo dục, vận động xây dựng nông thôn mới huyện thanh thủy, tỉnh phú thọPhát triển mạng lưới kinh doanh nước sạch tại công ty TNHH một thành viên kinh doanh nước sạch quảng ninhTrả hồ sơ điều tra bổ sung đối với các tội xâm phạm sở hữu có tính chất chiếm đoạt theo pháp luật Tố tụng hình sự Việt Nam từ thực tiễn thành phố Hồ Chí Minh (Luận văn thạc sĩ)Nghiên cứu, xây dựng phần mềm smartscan và ứng dụng trong bảo vệ mạng máy tính chuyên dùngNghiên cứu tổng hợp các oxit hỗn hợp kích thƣớc nanomet ce 0 75 zr0 25o2 , ce 0 5 zr0 5o2 và khảo sát hoạt tính quang xúc tác của chúngNghiên cứu khả năng đo năng lượng điện bằng hệ thu thập dữ liệu 16 kênh DEWE 5000Tìm hiểu công cụ đánh giá hệ thống đảm bảo an toàn hệ thống thông tinThơ nôm tứ tuyệt trào phúng hồ xuân hươngSở hữu ruộng đất và kinh tế nông nghiệp châu ôn (lạng sơn) nửa đầu thế kỷ XIXTăng trưởng tín dụng hộ sản xuất nông nghiệp tại Ngân hàng Nông nghiệp và Phát triển nông thôn Việt Nam chi nhánh tỉnh Bắc Giang (Luận văn thạc sĩ)Giáo án Sinh học 11 bài 14: Thực hành phát hiện hô hấp ở thực vật