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Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents

Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents

Isolation and identification of components from ixeris sonchifolia hance as potential anti stroke agents

... Evaluation of antioxidant activities of fractions to isolated from Ixeris sonchifolia Hance extract 44 2.4 Discussion and conclusions Chapter Isolation and characterization of components from ethyl ... Possible anti- coagulation activities of fractions to isolated from Ixeris sonchifolia Hance extract 42 2.3.2 Effects of fractions to isolated from Ixeris sonchifolia Hance extract on the viability of ... fraction of Ixeris sonchifolia Hance 48 55 3.1 Introduction 56 3.2 Materials and methods 56 3.2.1 Isolation of the fractions from Ixeris Sonchifolia Hance extract 56 III 3.2.1.1 Materials and general...
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Báo cáo khoa học:

Báo cáo khoa học: " Isolation and identification of a canine coronavirus strain from giant pandas (Ailuropoda melanoleuca)" ppt

... Therefore, isolation and identification of a potential viral pathogen was pursued Canine coronavirus was first isolated from a case of canine enteritis during an epizootic in Germany in 1971 Later, ... Howard J, Montali RJ, Hayek LA, Dubovi E, Zhang Z, Yan Q, Guo W, Wildt DE Serosurvey of ex situ giant pandas (Ailuropoda melanoleuca) and red pandas (Ailurus fulgens) in China with implications ... cytoblast of an infected FCWF cell The black arrow indicates the inclusion bodies of GPV in the cytoplasm of cells Scale bars = 200 nm Isolation and identification of a canine coronavirus strain from...
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báo cáo hóa học:

báo cáo hóa học:" Isolation and culture of fibroblasts from endoscopic duodenal biopsies of celiac patients" pdf

... immunocytochemistry of primary cells from duodenal endoscopic biopsies from celiac (upper panel) and non celiac (lower panel) patients; DAPI counterstained cellular nuclei The successful rate of cell cultures ... involvement of FBs in CD are actually obstructed by the absence of a specific models; we therefore aimed this study to isolate and culture primary FBs from endoscopic duodenal biopsies of CD and non-CD ... cellular nuclei panel) and non celiac (lower panel) patients; DAPI countercells from surface protein immunocytochemistry of (upper Fibroblast duodenal endoscopic biopsies from celiacprimary Fibroblast...
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Báo cáo khoa học:

Báo cáo khoa học: "Isolation and identification of Escherichia coli O157:H7 using different detection methods and molecular determination by multiplex PCR and RAPD" docx

... followed by Molecular determination of E coli O157:H7 15 Fig Result of multiplex PCR assay for detection of the Stx1 (614 bp), Stx2 (779 bp), eaeA (890 bp), and hlyA (165 bp) genes in E coli O157:H7 ... Comparison of the sensitivity of manual and automated immunomagnetic separation methods for detection of Shiga Molecular determination of E coli O157:H7 toxin-producing Escherichia coli O157:H7 ... applied for further determination by multiplex PCR and PCR for flagellar H7 antigen detection DNA preparation for Multiplex PCR, flagellar H7 PCR and RAPD analysis E coli O157:H7 strains which...
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Báo cáo y học:

Báo cáo y học: "Isolation and identification of bioactive compounds in Andrographis paniculata (Chuanxinlian)" potx

... expression of E-selectin, chitinases, mucin Muc5ac and iNOS in lung tissues and airway hyperresponsiveness [71] Andrographolide inhibits OVA-induced increases TNF-α and GM-CSF in BALF of OVA-sensitized ... namely andrographolide, bis-andrographolide 14-deoxy-11,12-didehydroandrographolide, andrograpanin, 14-deoxyandrographolide, (±)-5hydroxy-7,8-dimethoxyflavanone and 5-hydroxy-7,8dimethoxyflavone Andrographolide ... tract infections and relieving the inflammatory symptoms of sinusitis [85] The migratory response of leukocytes to chemokines forms the first line of defence to the invading microbial A paniculata...
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Isolation and characterization of allergens from curvularia lunata 1

Isolation and characterization of allergens from curvularia lunata 1

... Mala s 18 AJ 011 955 Mala s 17 AJ 011 956 Mala s AJ 011 957 Mala s 19 AJ 011 958 Mala s 37 AJ 011 959 Mala s 10 heat shock prot 70 86 AJ428052 Mala s 11 Mn superoxide dismut 23 AJ5484 21 Mala s 12 glucose-methanol-choline ... view of these, identification of various allergenic components of Curvularia will contribute to a better understanding of the repertoire of Curvularia allergens, and of the allergies caused by Curvularia ... AJ223 315 X85092 Chapter Asp f 11 peptidyl-prolyl isomeras 24 Asp f 12 heat shock prot P90 90 Asp f 13 alkaline serine protease 34 Asp f 15 16 AJ002026 Asp f 16 43 g3643 813 Asp f 17 AJ224865 Asp f 18 ...
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Isolation and characterization of allergens from curvularia lunata 2

Isolation and characterization of allergens from curvularia lunata 2

... 16 83 8 8 Curvularia pallescens 50 42 75 75 25 25 42 33 Curvularia fallax 42 33 42 75 33 25 25 25 Curvularia brachyspora 58 75 42 75 83 33 42 58 58 58 Curvularia lunata 75 42 58 92 25 33 58 Cladosporium ... SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 199 VIGEVTLDQVYGGAR SLVWEGSVLDSEEGIR 52. 129 ~10 8.84 76 27 .844 ~10 6. 92 √ 74 108.534 ~15 6. 12 √ 64 48 .21 4 ~45 6 .24 91 Chapter 21 7 21 8 22 6 22 8 22 9 23 0 Protein recA ... contigs 021 058 119 171 21 9 28 1 350 3 92 449 506 5 92 655 697 756 821 891 % Redundancy 12. 00 19.00 28 .66 32. 50 33.80 35.83 38. 42 38 .25 38.77 39 .20 41.50 42. 00 41.38 41.85 42. 47 43.13 Figure 2. 3: Percentage...
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Isolation and characterization of allergens from curvularia lunata 3

Isolation and characterization of allergens from curvularia lunata 3

... intensity of 20 3. 3 RESULTS AND DISCUSSION 3. 3.1 Cloning and Expression of the isolated putative C lunata allergens 3. 3.1.1 RACE amplification of the truncated putative allergen sequences Out of the ... 43. 4 28.8 25.6 33 .1 18.8 66.1 58.5 56.8 50.8 52.9 70.6 82.4 70.6 Cur l 13 C lunata A.fumigatus P.citrinum 14.5 32 .9 55 .3 40.8 25.0 35 .6 55.6 53. 1 59 .3 21.2 55.1 34 .7 70.6 35 .3 17.6 23. 5 128 Chapter ... Chapter 3. 1 INTRODUCTION In order to further understand and establish the allergenicity of the various putative allergens of Curvularia lunata, immunological characterization of the putative allergens...
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Isolation and characterization of allergens from curvularia lunata 4

Isolation and characterization of allergens from curvularia lunata 4

... (%) of the allergens tested over various populations Allergen ClSOD AfSOD ScSOD HsSOD Populations tested Singaporean Indian Italian 44 .7 23.5 44 .4 18 .4 47.1 1.3 57.9 52.9 45 .0 32.9 35.3 27.5 144 ... other SODs from human and fungi suggesting that the levels of SODs in the indoor 149 Figure 4. 5: 3D models of various MnSODs Monomeric and tetrameric (top view) subunit of the SOD is taken from Sabine ... (%) of the allergens tested over various populations Allergen ClHsp AfHsp ScHsp HsHsp Populations tested Singaporean Colombian 28.9 44 .1 61.8 54. 2 27.6 42 .4 56.6 50.8 175 Chapter Figure 4. 24: ...
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Isolation and characterization of allergens from curvularia lunata 5

Isolation and characterization of allergens from curvularia lunata 5

... Chapter 5. 1 DISCUSSION AND CONCLUSION Not many allergenic fungi are studied well and very few of them have been characterized for the allergens in detail Characterization of the fungus and its ... and critical residues for allergenicity, levels in the environment, function, etc Here, we report various allergens from Curvularia lunata For the rapid isolation of the allergenic proteins from ... of the allergens since it’s the three dimensional structure of the allergen which determines the interaction of the allergen to the IgE antibodies From the isolated C .lunata allergens, some of...
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isolation, selection and identification bacillus subtilis from muddreg of beer

isolation, selection and identification bacillus subtilis from muddreg of beer

... the subject of Bacillus subtilis isolated from waste water of beer and beer wallows in the brewing process Objective: Isolation, selection and identification of Bacillus subtilis from beer dregs, ... band of strains and Bacillus subtilis control had 1500bp Sequencing perform that strains (Bs4 and BN5) were Bacillus subtilis (99% and 98% identical), they present both of sample “mud-dreg” and ... aquaculture and especially in environmental treatment It helps disintegrate dreg, mud In this study”Isolation selection and identification of Bacillus subtilis from “mud-dreg of beer 21 strains of bacteria...
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Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

Tài liệu Báo cáo khoa học: Isolation and characterization of four type 2 ribosome inactivating pulchellin isoforms from Abrus pulchellus seeds docx

... Purification of four pulchellin isoforms from A pulchellus seeds Using a combination of affinity, ion exchange and chromatofocusing chromatography, four pulchellin isoforms were isolated from A pulchellus ... II, III and IV and refers to each pulchellin Characterization of four pulchellin isoforms isoform (P I, P II, P II and P IV) The A-chain of P II was formerly cloned and named recombinant pulchellin ... III and P IV isoforms from the eluate P III ⁄ P IV (Fig 1C) Isoelectric focusing gave pI of 5.8, 5.7, 5.5 and 5 .2 for the four isoforms respectively Secondary structure of the pulchellin isoforms...
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Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

Tài liệu Báo cáo khoa học: Phenol hydroxylase from Acinetobacter radioresistens S13 Isolation and characterization of the regulatory component docx

... aminoacids at the N-terminus of PHI (sequence: SKVYLALQDND) were compared with the sequences of the so called ‘intermediate components’ from two other PHs The N-terminal sequence of PHI from A radioresistens ... one PHR and one PHO (abc) protomer The hypothesis of a direct PHI phenol interaction is quite unlikely, because of the fact that the addition of phenol does not alter the emission spectrum of PHI ... oxygenase [28–30], and, in the case of Pseudomonas CF600 phenol hydroxylase, via a direct interaction with the substrate itself [9], in the case of A radioresistens S13 phenol hydroxylase, PHI...
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Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

Tài liệu Báo cáo khoa học: The isolation and characterization of cytochrome c nitrite reductase subunits (NrfA and NrfH) from Desulfovibrio desulfuricans ATCC 27774 Re-evaluation of the spectroscopic data and redox properties ppt

... describes the relationship between the heme core description and the spectroscopic and redox properties of each identified heme from the NrfHA complex Conclusions D desulfuricans ATCC 27774 ccNiR ... this communication, we report for the first time the isolation and biochemical characterization of D desulfuricans ATCC 27774 ccNiR subunits The stoichiometry between NrfH and NrfA is discussed The ... (2003) Cytochrome c nitrite reductase ˜ from Desulfovibrio desulfuricans ATCC 27774 The relevance of the two calcium sites in the structure of the catalytic subunit (NrfA) J Biol Chem 278, 17455–17465...
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