identification and interpretation of clusters from dendrogram
Báo cáo hóa học: " Identification and characterization of alkaline serine protease from goat skin surface metagenome" docx
... mM EDTA, 1.5 M NaCl), in the presence of 0.1 mg of proteinase K and mg of lysozyme and incubated at 37°C for 30 Lysis was completed by adding ml of 20% SDS and incubated for h at 65°C with shaking ... Randomly selected transposon carrying protease negative mutants were sequenced and alignment of these sequences lead to the identification of the protease open reading frame (ORF) Analysis of ... 65 and 85% of the maximum activity at the temperature range of 35°C and 55°C respectively Thermal stability of the purified AS-protease was estimated at Figure Multiple sequence alignment of...
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Báo cáo khoa học: " Identification and prevalence of Ehrlichia chaffeensis infection in Haemaphysalis longicornis ticks from Korea by PCR, sequencing and phylogenetic analysis based on 16S rRNA gene" docx
... collected from grass vegetation, from cattle and horse ranches and from different animals such as cattle, horse, dogs and rodents (data not shown) **Ticks were collected from rice fields and army ... buffer, 2.5 µl of 25 mM MgCl , µl of 2.5 mM deoxynucleoside triphosphate (dNTPs), 2.5 U of Taq-polymerase (Promega, USA), pmol of each primer, EEC and ECB (Genotech, Korea), and 200 ng of template ... Most of the ticks (896/1,288) investigated in this study originated from the rice fields and army training sites of Gyeonggi province Other ticks were collected from grass vegetation and cattle and...
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báo cáo khoa học: " Identification and characterisation of seed storage protein transcripts from Lupinus angustifolius" docx
... contains eight distinct epitopes and most of these differ from the corresponding regions of other legume and tree-nut allergens [41] Identification of specific allergens and their IgE binding epitopes ... increase started from 4-8 DAA and in most cases the maximum was reached between 33 and 38 DAA Proteomic identification of NLL conglutins and IgE binding conglutins With the availability of full-length ... Vierucci A, Duranti M: One- and two-dimensional electrophoretic identification of IgE-binding polypeptides of Lupinus albus and other legume seeds Journal of Agricultural and Food Chemistry 2005,...
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Báo cáo y học: " The identification and management of ADHD offenders within the criminal justice system: a consensus statement from the UK Adult ADHD Network and criminal justice agencies" doc
... development and provision of accessible information and resources for staff and patients and their families 2) The development and provision of a monitoring checklist to record assessment and prescription ... organisation of effective court liaison and diversion arrangements and the services needed to support them The focus was expanded to include a more comprehensive consideration of the ‘offender pathway’ and ... Training and Education and Community Payback) and monitor their clients’ progress and, while there are national standards, each Trust and is encouraged to tailor responses to local needs and priorities...
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Báo cáo y học: "Identification and isolation of Genotype-I Japanese Encephalitis virus from encephalitis patients" pot
... PrM and E genes (Figure 1) GZ56 strain showed high homology with JEV GI strains obtained from swine of Japan (Mie/40) and China (HEN0701) in nt (99.2% and 98.2% respectively) and aa (99.8% and ... careful review of the manuscript and useful suggestions This work was supported by grants from the Ministry of Science and Technology, People’s Republic of China (no.2003BA712A08-01 and 2009ZX10004-202); ... HYW and QT participated in the serological studies, virus isolation, and the design of the study GDL conceived of the study, and participated in its design and coordination All authors read and...
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báo cáo khoa học: " Identification and characterisation of CYP75A31, a new flavonoid 3’5’-hydroxylase, isolated from Solanum lycopersicum" pdf
... Page 12 of 12 38 Slimestad R, Verheul MJ: Review of flavonoids and other phenolics from fruits of different tomato (Lycopersicon esculentum Mill.) cultivars Journal of the Science of Food and Agriculture ... were sown on rock wool and given Hoagland nutrient solution containing 15 mM NO [31] RNA and DNA used to identify coding sequence and introns of the F3’5’H gene was isolated from plants grown in ... (forward and reverse) and μl cDNA Standard cycling conditions (2 at 50°C, 10 at 95°C and 40 cycles altering between 15 s at 95° C and at 60°C) were used for product formation Forward and reverse...
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Báo cáo y học: "Identification and isolation of embryonic stem cells in reproductive endocrinology: theoretical protocols for conservation of human embryos derived from in vitro fertilization" ppsx
... be derived from subsequent isolation and re-plating of the ICM cells, which are then screened for stemness by a variety of recognized markers Once in stable culture, ESCs are capable of either ... originated from the intact/source embryo group and the single blastomere culture group were disaggregated from their respective blastocysts and re-plated on to fresh feeder cells for confirmation and ... blastomeres obtained from the dead embryos failed to progress, a few ICM-like clusters developed from single blastomeres These were isolated (as were ICMs derived from the intact blastocysts) and disaggregated...
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Identification and characterization of novel anticoagulants from bungarus fasciatus venom
... Anticoagulant activity of pooled fractions Chapter Three Figure 3.1: Purification of BF-AC1 and BF-AC2 from the venom of B fasciatus Figure 3.2: ESI-MS profile of BF-AC1 (A) and BF-AC2 (B) Figure ... Discussion and Conclusion Chapter Identification and characterization of novel inhibitors on extrinsic tenase complex from Bungarus fasciatus (banded krait) Venom 3.1 Introduction 3.2 Materials and ... Kang Tse Siang, Professor R Manjunatha Kini and Associate Professor Go Mei Lin for their constant encouragement and scientific input throughout my candidature Dr Kang and Prof Kini have provided...
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Identification and characterization of novel proteins from a rare australian elapid snake drysdalia coronoides
... 41 Materials and Methods 42 iii Reagents and kits; Collection of venom and venom gland; RNA isolation and cDNA synthesis; Cloning of ds cDNA; Isolation of plasmids and verification of clones; cDNA ... goes to our collaborator Prof Daniel Bertrand from Department of Neuroscience, University of Geneva, Geneva, Switzerland for carrying out a part of pharmacological studies of drysdalin in his laboratory ... walking and BLASTn results of LNTx genes from D coronoides 169 5.4 Cartoon representations of results obtained from genome walking of LNTx genes from D coronoides 172 Chapter 6.1 Comparison of partial...
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Identification and characterization of novel group 5 and group 21 allergens from dust mite and ige binding epitope mapping of blo t 5
... commonly derived from 10 pollen, fungi, mites, endothelial tissues and dander from pets, venom from insects and foods such as egg, milk, fruits, nuts and fish (Kerkhof et al., 2003; Burge and Rogers, ... Cross-reactivity of Group and Group 21 allergens 153 6.2.5.1 6.3 153 6.2.5.2 6.2.6 Cross-reactivity of Blo t and Blo t 21 Cross-reactivity of Der f and Der f 21 156 Cross-reactivity of Der p and Lep d ... (C) and between Sui m 5.01 and Sui m 21 (D) 145 Figure 6.5 SDS-PAGE profile of a panel of Group and Group 21 proteins including Ale o 5, Der p 5, Lep d and Gly d 148 Figure 6.6 Frequencies of...
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Identification and characterisation of a mads box gene from rafflesia cantleyi solms laubach (rafflesiaceae)
... MADS1 from Populus tomentosa, MPF2 and MPF4 from Physalis pubescens, IbMADS4 from Ipomoea batatas, StMADS11 and StMADS16 from Solanum tuberosum, and SVP and AGL24 from Arabidopsis thaliana, all from ... RcMADS1 from Rafflesia cantleyi; MPF2 and MPF3 from Physalis pubescens; AGL24 and SVP from Arabidopsis thaliana; and StMADS16 and StMADS11 from Solanum tuberosum 39 As this cDNA had been amplified from ... understanding of the development of highly specialised flowers of Rafflesia, and parasitic plants in general 17 CHAPTER MATERIALS AND METHODS 3.1 Plant materials Flower buds of various sizes of Rafflesia...
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Identification and segmentation of the central sulcus from human brain MR image
... the anatomy and radiology of the CS, and the MRI techniques are briefly described It also reviews the trends and recent development of the methods and the history of the identification of the CS ... poor temporal memory and altered sexual behavior Segmentation and identification of the CS is, therefore, crucial 1.3 Objective of Research The aim of this project is to design and develop an algorithm ... disturbance of motor function (loss of fine movements and strength, poor voluntary eye gaze and corollary discharge), environmental control of behavior (risk taking and rule breaking), loss of divergent...
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Prevention and Management of Complications from Gynecologic Surgery pdf
... clindamycin and gentamicin, clindamycin and ciprofloxacin, clindamycin and aztreonam, metronidazole and gentamicin, or metronidazole and ciprofloxacin.26 In patients with known history of MRSA infection ... discussion of diagnostic and therapeutic treatment options, and of the spectrum of adverse events that may occur with each, appropriately adjusted to fit the risk profile of the patient a Department of ... preventing and managing complications from gynecologic surgery William F Rayburn, MD, MBA Department of Obstetrics and Gynecology University of New Mexico School of Medicine MSC10 5580, University of...
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The Cavalier Songs and Ballads of England from 1642 to 1684 Edited doc
... never-to-be-superseded topics of love, war, and wine The people of our day have little heart or appreciation for song, except in Scotland and Ireland England and America are too prosaic and too busy, and the masses, ... Satires in the form of a litany were common from 1646 to 1746, and even later From an extempore prayer and a godly ditty, From the churlish government of a city, From the power of a country committee, ... altar, From oaths and covenants, and being pounded in a mortar, From contributions, or free-quarter, Libera, etc From mouldy bread, and musty beer, From a holiday's fast, and a Friday's cheer, From...
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Báo cáo khoa học: Thermodynamic stability and folding of proteins from hyperthermophilic organisms doc
... Comparisons of the thermodynamics and kinetics of the folding of proteins from mesophilic and thermophilic ⁄ hyperthermophilic organisms can provide an insight into the mechanisms of stabilization ... decreases from 79 to 20 kJÆmol)1 when the pH drops from to 2.5 [15] Analysis of a structural model of AmFd suggests that a combination of additional surface ion pairs, the zinc cofactor, and an ... calorimetry and chemical denaturation of Sac7d J Mol Biol 264, 784–805 43 Zeeb M, Lipps G, Lilie H & Balbach J (2004) Folding and association of an extremely stable dimeric protein from Sulfolobus islandicus...
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Báo cáo khoa học: "Examining the Role of Linguistic Knowledge Sources in the Automatic Identification and Classification of Reviews" doc
... work of Koppel and Schler (2005), we identify and extract objective material from nonreviews and show how to exploit such information in polarity classification Related Work 2.1 Review Identification ... represent term polarity and introduce a variety of numerical features that are aggregate measures of the polarity values of terms selected from the document under consideration Review Identification Recall ... dataset Briefly, a positive review has a rating of ≥ 3.5 (out of 5) or ≥ (out of 4), whereas a negative review has a rating of ≤ (out of 5) or ≤ 1.5 (out of 4) 12 The curves are not shown due to space...
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Báo cáo khoa học: "Using Conditional Random Fields to Extract Contexts and Answers of Questions from Online Forums" docx
... candidate pairs with high probability of being context and answer of a question To introduce skip-chain edges between any pairs of non-contiguous sentences will be computationally expensive, and ... answering In Proceedings of ACL J Huang, M Zhou, and D Yang 2007 Extracting chatbot knowledge from online discussion forums In Proceedings of IJCAI J Jeon, W Croft, and J Lee 2005 Finding similar ... Wan and K McKeown 2004 Generating overview summaries of ongoing email thread discussions In Proceedings of COLING Z Wu and M S Palmer 1994 Verb semantics and lexical selection In Proceedings of...
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Báo cáo Y học: Identification and characterization of thioredoxin and thioredoxin reductase fromAeropyrum pernixK1 pdf
... dithiothreitol in a total volume of 50 lL Then, 40 lL of a reaction mixture composed of 200 lL of Hepes (1 M), pH 7.6, 40 lL of EDTA (0.2 M), 40 lL of NADPH (40 mgÆmL)1), and 500 lL of insulin (10 mgÆmL)1) ... Molecular mass determination of ApTrx and ApTR Molecular masses of recombinant ApTrx and ApTR were determined by analysis of the elution files of standard proteins from a Sephacryl S-200 HR 26/60 ... concentration of 1.2 mM The formation of NADP+ and NADPH was followed from the decrease and increase at 340 nm, respectively Fig Reduction of insulin catalyzed by thioredoxin from A pernix and E coli...
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Recommended code of practice for the care and handling of poultry from hatchery to processing plant ppt
... for Care and Handling of Pigs (1984); Recommended Code of Practice for the Care and Handling of Special Fed Veal Calves (1988); Recommended Code of Practice for the Care and Handling of Mink (1988); ... Recommended Code of Practice for the Care and Handling of Ranched Fox (1989) This code is an updated version of the 1983 publication on the care and handling of chickens, and has been expanded to include ... Facilities for and handling of loosehoused poultry 5.3 Catching and loading 5.4 Transport 29 30 30 32 Section Processing plants 34 6.1 Receiving and handling of poultry 6.2 Slaughter of poultry 34...
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structure and interpretation of computer programs
... places of accuracy can we represent? Is the range of integers the same as the range of real numbers? Above and beyond these questions, of course, lies a collection of issues concerning roundoff and ... together the power and elegance of Lisp and Algol From Lisp we take the metalinguistic power that derives from the simple syntax, the uniform representation of programs as data objects, and the garbage-collected ... evaluates the elements of the combination and applies the procedure (which is the value of the operator of the combination) to the arguments (which are the values of the operands of the combination)...
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Tìm thêm: xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản khảo sát chương trình đào tạo gắn với các giáo trình cụ thể tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ phát huy những thành tựu công nghệ mới nhất được áp dụng vào công tác dạy và học ngoại ngữ mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ hệ số công suất cosp fi p2 đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 đặc tuyến tốc độ rôto n fi p2 sự cần thiết phải đầu tư xây dựng nhà máy phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng 9 tr 25