isolation and culture of neurons and astrocytes from the mouse brain cortex
Báo cáo y học: "Genome-wide expression profiling and bioinformatics analysis of diurnally regulated genes in the mouse prefrontal cortex" pps
... studied in the whole cortex, cerebellum, basal forebrain, and hypothalamus in the rat [25,26] and the mouse [27], and in fly heads [24,28,29] However, these studies assayed only limited numbers of genes, ... (the rat RGU34A arrays), 752 (4.9%) of the transcripts in the whole cortex and 223 (4.8%) of the transcripts in the cerebellum were identified as regulated by sleep/wakefulness independent of ... our list of mouse diurnal genes with the mouse orthologs of the genes reported by Cirelli et al [25], we found 75 common genes in the sleep-related cortex, 124 in the wakefulness-related cortex, ...
- 15
- 299
- 0
Báo cáo khoa học " Study on macrophage activation and structural characteristics of purified polysaccharide from the liquid culture broth of Cordyceps militaris " doc
... exhibit a random coil conformation similar to that of dextran 3.6 Identification of anomeric configuration Fig Isolation and purification of polysaccharides extracted from the liquid culture broth of C ... fractions The yield of the neutral fraction (CPSN) and the acidic fraction (CPSA) obtained from the crude polysaccharide extract CPS was 0.328 g/g and 0.034 g/g, respectively (Fig 1A) The molecular ... for at 70 ◦ C, then mixed with a 5× first-strand buffer, 10 mM dNTPs, and 0.1 M DTT The reaction mixture was further incubated for at 37 ◦ C, then for 60 after the addition of U of MuLV reverse...
- 7
- 492
- 7
Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka
... expression of 13 Chapter I Introduction oct4 is regulated by the changes of DNA methylation The statue of DNA methylation is related to the changes of the chromatin structure of the mouse oct4 gene and ... characterization of two isoforms of the medaka stat3 104 109 3.2.1 Isolation of stat3 cDNAs from the medaka 109 3.2.2 Medaka Stat3 produces two isoforms Stat3a and Stat3b 112 3.2.3 The medaka stat3 ... Isolation and characterization of stem cell regulatory genes oct4 and stat3 from the model fish medaka LIU RONG (Master of Biological Sciences) A THESIS SUBMITTED FOR THE DEGREE OF DOCTOR OF...
- 170
- 340
- 0
Tài liệu Báo cáo Y học: Expression and characterization of active site mutants of hevamine, a chitinase from the rubber tree Hevea brasiliensis docx
... hevamine, the oxygen atom of the N-acetyl group of the )1 sugar is positioned close to the C1 atom of the )1 sugar, and is hydrogen bonded to Tyr183 Asp125 makes a hydrogen bond to the nitrogen atom of ... and the Tyr183 hydroxyl group, and between its amide nitrogen atom and Asp125 In the mutants, the hydrogen bond of the amide nitrogen with the Asp125 side chain is not possible anymore, and the ... judged by SDS/ PAGE The activity of the pure recombinant protein was 80% of that of the wild-type protein in both the lysozyme and chitinase assays Attempts to further purify the recombinant hevamine...
- 9
- 616
- 0
AKBAR, EMPEROR OF INDIA A PICTURE OF LIFE AND CUSTOMS FROM THE SIXTEENTH CENTURY ppt
... kinsmen, plundering of cities, devastation of the lowlands and the slaughter of thousands of men, women and children of the predecessor's adherents; for five centuries northwest and northern India literally ... heels from the terrace of the palace to the courtyard below The horrible deed was done but the wretch was not dead Then the Emperor commanded the shattered body of the dying man to be dragged up the ... to brand Islam and its supreme contempt for followers of other faiths, with one of the greatest stains in the history of humanity When a tax-collector gathered the taxes of the Hindus and the...
- 38
- 673
- 1
Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and trifluoromethionine, a promising lead compound against amoebiasis ppt
... concentration of mm) and the kinetic parameters of recombinant EhMGL1 and EhMGL2 (Tables and 2) Despite the heterogeneity of the EhMGL1 preparation used in the previous study [13], the kinetic constants of ... in the Km and a 58% decrease in the kcat for Cys This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination and c-elimination of the C–S bond, but not the ... in mouse and hamster models [26] (Kobayashi and Nozaki, unpublished results) The limited presence of MGL among organisms, and the remarkable differences in the toxicity of TFM against amoeba and...
- 13
- 406
- 0
Báo cáo khoa học: The fabp4 gene of zebrafish (Danio rerio) ) genomic homology with the mammalian FABP4 and divergence from the zebrafish fabp3 in developmental expression pot
... Alignment of the cDNA sequence with the coding sequence of zebrafish fabp4 showed one nucleotide difference in the coding region of exon 2, resulting in an alteration of the ninth codon of this exon from ... Comparison of the gene structure of zebrafish fabp4 and its chicken and mammalian orthologous genes Exons are shown as solid boxes, introns as open boxes, and the UTRs of the first and fourth exon ... sequences of members of the FABP family from mammals and fishes (Fig 4) A distinct clade consisting of zebrafish FABP4, the four Antarctic fish H6-FABPs and putative FABP4 homologs from three other teleost...
- 13
- 478
- 0
Báo cáo khoa học: Molecular and functional characterization of novel CRFR1 isoforms from the skin pptx
... between the fully active alpha isoform and other variants, we conducted a series of cotransfection experiments and compared ligand-induced accumulation of cAMP (Fig 3) Although the level of cAMP ... assembled by PCR of these two fragments with primers E3 and E11 Two different constructs were produced for CRFR1h isoform The first contained exons 1–4 and a fragment of the cryptic exon up to the translation ... (5¢-GGTAGTGCACCTTGCTTTTTTTCTCTCCCCA3¢) and attached to another fragment of CRF receptor in the final PCR by primers E3 and E11 To attach V5 epitope to the CRFR1a, g, h2 and e2 isoforms we amplified the corresponding...
- 10
- 671
- 0
Báo cáo khoa học: Molecular cloning, expression analysis and functional confirmation of ecdysone receptor and ultraspiracle from the Colorado potato beetle Leptinotarsa decemlineata pdf
... the difference of structures of the ligand binding pocket In the other study, we demonstrated that the molting hormone activities of ecdysone agonists measured in cultured integument system of ... decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ecdysone agonists to the in vitro ... 6.29 and L migratoria USP showed higher homology to RXR of human and mouse than USP isoforms of dipteran and lepidopteran insects Thus, the functions of USP of L decemlineata, T molitor and L...
- 15
- 564
- 0
Báo cáo khoa học: Structural and mutational analysis of TenA protein (HP1287) from the Helicobacter pylori thiamin salvage pathway – evidence of a different substrate specificity doc
... shows 33% identity and 51% similarity to the TenA protein from B subtilis The 3D structure of the monomer is quite similar to that of the other members of the TenA family of known structure: ... The superposition of the Ca atoms of one monomer with that of the other members ˚ of the family gives a rmsd of 1.7, 1.3 and 1.4 A for models 1RTW, 1UDD and 1TYH, respectively The major differences ... Cys135 adds to C6 of the pyrimidine ring, favoring the exit of the aminic group The subsequent addition of a water molecule and the expulsion of the active cysteine complete the reaction Asp44...
- 9
- 491
- 0
Báo cáo khoa học: Identification, cloning and characterization of two thioredoxin h isoforms, HvTrxh1 and HvTrxh2, from the barley seed proteome pot
... proteins The resulting Vmax and Km values for HvTrxh1 were 2.5 nmolặs)1ặnmol)1 and 13 lM Again, HvTrxh2 and TrxTa resembled each other with Vmax of 2.0 and 1.5 nmolặs)1ặnmol)1 and Km of 44 lM and ... and HvTrxh2 are mainly at the N-termini and C-termini of the proteins, and they are mostly located away from the active site in the modelled 3D structure However, R101 of HvTrxh1, replaced by ... spots 296 and 297) and HvTrxh2 (TC45680; spot 313) Theoretical tryptic digests of these sequences were used to determine the sequence coverage obtained from peptide mass mapping of the three...
- 11
- 435
- 0
Báo cáo khoa học: H NMR study of the molecular structure and magnetic properties of the active site for the cyanomet complex of O2-avid hemoglobin from the trematode Paramphistomum epiclitum pdf
... away from the heme normal (z axis), a is the angle between the projection of the tilt of the z axis on the xÂ, y plane (dened direction of tilt of z and the x axis), and j % a + c denes the ... of WT and the other of rWT metHb, exhibit differences that include important portions of the protein that we have characterized above Thus parts of the FG corner move away from the heme and the ... The two structures of WT metHb and rWT metHbH2O, exhibit signicant differences in the interaction of the FG loop with the heme and in the position of the B-helix, with Tyr32(B10) closer to the...
- 14
- 504
- 0
Internet Banking and the question of Bank Run: lesson from the Northern Rock Bank case pdf
... the need for a lender of last resort as underlined Rochet and Vives (2004) Indeed the creation of the lender of last resort facility and the deposit insurance in most of the countries since the ... decline of the shares’ price during the first part of the year, there had not been any sign of depositors’ massive withdrawals before September This is a further proof that despite the existence of ... branches formed, the length of the queue being aggravated by the break down of the bank website and its call center The reason for the initial panic has to be found in the type of deposit insurance...
- 7
- 541
- 0
CAN AESTHETIC THEORIES OF ART BE RESCUED FROM THE PROBLEM OF AVANT-GARDE AND OTHER NON-PERCEPTUAL ARTWORKS? – AN EXPLORATION OF NON-PERCEPTUAL AESTHETIC PROPERTIES pdf
... avant-garde artworks in the domain of the aesthetic Since the era of Duchamp’s Fountain, avant-garde artworks have developed to the point of once again challenging the aesthetic theory of art Counterexamples ... (S) and proposes the third solution’ as a way to incorporate avant-garde artworks into the aesthetic theory of art The third solution invokes the idea of non-perceptual aesthetic properties and ... works thus removing the majority of counterexamples to the aesthetic theory of art However, the nature of avant-garde art means that since the era of Duchamp’s readymade artworks, the boundaries have...
- 11
- 505
- 0
university of minnesota press high techne art and technology from the machine aesthetic to the posthuman nov 1999
... Introduction: The Question concerning High Tech 1 The Spirit of Utopia and the Birth of the Cinematic Machine 23 The Mediation of Technology and Gender 48 The Avant-Garde Techne ¯ and the Myth of Functional ... through the process of repression” (p 241) Indeed, those instances that evoke the sense of the uncanny the idea of the double, ghosts, the return of the dead, the evil eye, the coming to life of machines ... contingent They not, as in the experience of the aura, conjure up the spirit of the beautiful; they not carry the mystical significance and internal purposiveness of the “living” symbol They are, rather,...
- 204
- 356
- 0
Báo cáo sinh học: " Clearance of an immunosuppressive virus from the CNS coincides with immune reanimation and diversification" pptx
... infection with × 106 PFU of clone 13 Note the minimal amount of virus in the brain at day 10 and the complete inundation of the brain parenchyma by day 30 During the late phase of persistence (day ... were bred and maintained in a closed breeding facility at The Scripps Research Institute The handling of all mice conformed to the requirements of the National Institutes of Health and The Scripps ... dramatic reduction in the number of persistently infected astrocytes and the eventual eradication of clone 13 from the CNS These data provide a framework for understanding the cellular constituents...
- 16
- 378
- 0
Báo cáo hóa học: " Clearance of an immunosuppressive virus from the CNS coincides with immune reanimation and diversification" pptx
... infection with × 106 PFU of clone 13 Note the minimal amount of virus in the brain at day 10 and the complete inundation of the brain parenchyma by day 30 During the late phase of persistence (day ... were bred and maintained in a closed breeding facility at The Scripps Research Institute The handling of all mice conformed to the requirements of the National Institutes of Health and The Scripps ... dramatic reduction in the number of persistently infected astrocytes and the eventual eradication of clone 13 from the CNS These data provide a framework for understanding the cellular constituents...
- 16
- 389
- 0
Báo cáo hóa học: " Algorithms for Blind Components Separation and Extraction from the Time-Frequency Distribution of Their Mixture" docx
... take a slice of the TFD T(t, f ) (2) Search and count the number of peaks in each slice (3) Evaluate the histogram of the obtained set of peaks numbers (4) Estimate the total number of the signal ... of peaks of the TFD slice T(t0 , f ) By searching and counting the peaks of each TFD slice, we end up with a set of numbers The number corresponding to the maximum of the histogram of these numbers ... order to group the halves (or parts) of the actual components together This is performed in the third and last phase of the algorithm Algorithm gives the details of the second phase The classification...
- 9
- 182
- 0
Báo cáo lâm nghiệp: "ISSR and AFLP identification and genetic relationships of Chinese elite accessions from the genus Populus" ppt
... profile using any one of the ISSR and AFLP primers Therefore, any of the primers could be used separately to identify these cultivars in the future ISSR and AFLP fingerprinting of poplar 503 Figure ... analyzed (129 for ISSR and 252 for AFLP) This possibility reinforces the importance of the number of fragments and their coverage of the overall genome Alternatively, it could be that the two marker ... techniques vary in the way that they resolve genetic differences, in the type of data they generate and in the taxonomic levels at which they can be most appropriately applied The AFLP and ISSR analysis...
- 8
- 425
- 0
Báo cáo khoa học: "Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus" doc
... citatsomreht a ot detcennoc )nedewS ,aicamrahP( tinu IIrohpitluM eht ni demrofrep saw sisylana citerohportcele ehT la te gnisucof cirtceleosi lacitylanA snoitacifidom thgils htiw ]42[ nibwoT fo dohtem ... ,BKL( rettolb yrd-imes tolBavoN a gnisu demrofrep saw sisylana tolbonummI la te gnittolbonummI ]31[ ilmmeaL yb debircsed snoitidnoc eht ot gnidrocca demrofrep saw siserohportcele dna ,selpmas ,sleg ... noitaraperP )ASU ,daRoiB( lleC balS-lauD II naetorP -iniM eht ni demrofrep saw sisylana citerohportcelE EGAP-SDS ]3[ dohtem drofdarB eht yb detamitse saw sPLG eht fo tnetnoc nietorp ehT fo sPLG...
- 6
- 267
- 0
Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các nguyên tắc biên soạn xác định thời lượng học về mặt lí thuyết và thực tế điều tra đối với đối tượng giảng viên và đối tượng quản lí điều tra với đối tượng sinh viên học tiếng nhật không chuyên ngữ1 khảo sát thực tế giảng dạy tiếng nhật không chuyên ngữ tại việt nam khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn các đặc tính của động cơ điện không đồng bộ đặc tuyến hiệu suất h fi p2 đặc tuyến mômen quay m fi p2 động cơ điện không đồng bộ một pha thông tin liên lạc và các dịch vụ phần 3 giới thiệu nguyên liệu từ bảng 3 1 ta thấy ngoài hai thành phần chủ yếu và chiếm tỷ lệ cao nhất là tinh bột và cacbonhydrat trong hạt gạo tẻ còn chứa đường cellulose hemicellulose chỉ tiêu chất lượng theo chất lượng phẩm chất sản phẩm khô từ gạo của bộ y tế năm 2008 chỉ tiêu chất lượng 9 tr 25