Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity

... Santoro SA 1995 ;The spatial and temporal expression of the alpha beta integrin and its ligands, collagen I, collagen IV, and laminin, suggest important roles in mouse mammary morphogenesis Differentiation ... and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 ... crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving the expansion and neuronal differentiation of mesenchymal stem cells...

Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps

... embryogenesis and morphogenesis of various tissues and organs, where they regulate growth, differentiation, chemotaxis and apoptosis of a variety of cell types, including mesenchymal, epithelial, ... compare the effects of BMP-4 and BMP-2 expression on chondrogenesis of primary MSCs and to investigate whether levels and extent of hypertrophy in vitro is influenced by the choice of transgene ... and CS4 and the quantitative GAG assay, indicating increased GAG levels at days 14 and 21 in the BMP-2-modified aggregates Notably, chondrogenic differentiation induced by either transgene increase...

Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx

... 10 of 10 doi:10.1186/1423-0127-17-56 Cite this article as: Lin et al.: Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating ... of Akt and ERK for stage III cells There was a baseline of Akt and ERK phosphorylation without adding ECM FN and LAM increased phosphorylation of Akt and ERK, and LAM had greater effects on Akt ... mechanism of pancreatic islets FN and LAM enhances activation of Akt and ERK The ECM bind to cells by activating signaling molecules such as Akt and ERK Therefore, we analyzed the effect of FN and...

Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1

... Induction Upon Biaxial Bioreactor Stimulation of Mesenchymal Stem Cells and Endothelial Progenitor Cells Cocultures in 3D Honeycomb Scaffolds for Vascularised Bone Tissue Engineering Tissue Engineering ... success of the tissue engineering strategy (Figure 1- 1) Cells and Biomolecules Scaffolds TISSUE Bioreactors Imaging Tools Figure 1- 1: Four key technologies used in tissue engineering include cells and ... 11 1. 7 .1 Vascularised BTE – A Mimicry of Natural Bone Tissue .11 1. 7.2 Aims and Hypotheses 12 1. 7.2 .1 Main Aim 12 1. 7.2.2 Hypothesis 13 1. 7.2.3 Hypothesis...

Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf

... 19 The relative increase in expression of the osteogenic markers in the young donor, as a result of chordin knockdown, was within the range of that of the donors over 70 years old MSCs from young ... analysis of flow cytometry data shows the transfection efficiency of siRNA The darker peak represents the fluorescence-positive cell population, which is clearly shifted from the position of the ... [above]) The relative number of cells was determined spectrophotometrically entiation of human mesenchymal transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human...

báo cáo khoa học: "Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP" pptx

... Mondalek et al., Inhibition of angiogenesis- and inflammation-inducing factors in human colon cancer cells in vitro and in ovo by free and nanoparticle-encapsulated redox dye, DCPIP Journal of Nanobiotechnology ... Detection of angiogenesis- and inflammation-inducing factors by Western blot analysis HCT116 cells were treated with empty PLGA NPs (control), DCPIP, and DCPIP NPs for 48 hr, then, aliquots of 35 μg of ... IL-6 and IL-8, in cultured human colon cancer cells; (c) DCPIP and DCPIP NPs are similarly effective in Page of Figure Genetic Expression and Measurement (GEM) assay Detection of involvement of...

Báo cáo y học: "Therapeutic potential of human umbilical cord mesenchymal stem cells in the treatment of rheumatoid arthritis" pps

... using the corresponding inhibitors They included 1-MT (1 mM), an inhibitor of IDO enzymatic activity, INDO (5 μM), an inhibitor of PGE2 synthesis, L-NAME (1 mM), a specific inhibitor of NO synthase, ... Notably, delayed addition of UC-MSCs maintained such inhibitory effects, suggesting that the transplantation of these cells is practicable and effective for treatment of RA Interestingly, the invasive ... could partly explain the absence of inflammatory infiltrates in the synovium of mice treated with human UC-MSCs Moreover, UC-MSCs increased the levels of the antiinflammatory cytokine IL-10 Aside...

Báo cáo y học: "Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells" pot

... article as: Purmessur et al.: Notochordal conditioned media from tissue increases proteoglycan accumulation and promotes a healthy nucleus pulposus phenotype in human mesenchymal stem cells Arthritis ... PA USA) at 20 × magnification Glycosaminoglycan (GAG) and DNA content Mass spectrometry and data analysis To examine GAG and DNA in the cell pellet, spent media was removed and 200 μl of lysis ... Microarray analysis of rat, bovine and canine IVD tissue has identified several candidate phenotypic markers such as Glypican, Biglycan, Keratin 19 and Laminin B1 [22,23] However, studies have also...

Occupational therapy interventions and physical activity interventions to promote the mental wellbeing of older people in primary care and residential care pdf

... health guidance 16 Occupational therapy interventions and physical activity interventions to promote the mental wellbeing of older people in primary care and residential care Ordering information You ... the mental wellbeing of older people This guidance focuses on the role of occupational therapy interventions and physical activity interventions in the promotion of mental wellbeing for older people ... fieldwork findings The full fieldwork report Occupational therapy and physical activity interventions to promote the mental wellbeing of older people in primary care and residential care: fieldwork...

Báo cáo y học: "ntravenous transplantation of allogeneic bone marrow mesenchymal stem cells and its directional migration to the necrotic femoral head"

... intravenously injected into nude mice and the distribution and migration of MSCs were dynamically monitored to evaluate the feasibility and safety of intravenous implantation of allogeneic MSCs in the ... transplanted the bone marrow stem cells into the necrotic femoral heads, and results show bone marrow stem cells can remove vascular lesions and promote angiogenesis in necrotic femoral heads, ... intravenously implanted allogeneic MSCs could directionally migrate to femoral heads, and survive especially in the necrotic femoral heads, the mechanisms underlying the directional migration of MSCs...

Comparing the cultural and linguistic analysis of the English word “meal” and words relating to it in contrast with Vietnamese equivalents.

... of word meal in English and words relating to it (in contrast with Vietnamese equivalents) - Definition of word meal - Field of word meal in English and in Vietnamese equivalents - Cultural and ... with the notion of meal are called field of word meal In our opinion, field of word meal is all the words relating to it mainly including eating and drinking They are parts of the word meal and ... _ Compare the cultural and linguistic analysis of the English word meal and words relating to it in contrast with Vietnamese equivalents Scope of the study Because of space and time, the graduation...

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Eutocius’ Commentary to On the Sphere and the Cylinder I

... and went on to discuss the expression on the same side” and its application for the deﬁnition of “concave in the same direction.” Only the diagram for 245 to t h e d e f i n i t i on s Γ N K ... that lines concave in the same direction are identiﬁed, it is possible to take, on some line, concave in the same direction, two chance points, so that the line joined to them will fall on neither ... becomes £ in Eutocius; the word order in the phrase the straight lines joining their limits” differs slightly between the two versions Point shows that Eutocius is willing to change the original...

Eutocius’ Commentary to On the Sphere and the Cylinder II

... , so the on the second;280 (3) therefore it is: as P to on P to the on K (4) But as the on P to the on K, so the on K to the on , so the ; ... )) 266 to “Therefore as P to , the on K to the on ” (1) For since it is: as P to K, K to , (2) therefore also: as the first to the second, so the on the first to the ... as the on A to the on AB, so the on B to the on BE (6) But as the on A to the on AB, so A to AE;242 (7) for as the first to the third, so the...

Tài liệu Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrho pdf

... Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae In order to contain the spread and impact of gonococcal infections and AMR, the following ... Global action plan to control the spread and impact of antimicrobial resistance in Neisseria gonorrhoeae WHO Library Cataloguing -in- Publication Data Global action plan to control the spread and ... plasmid-mediated resistance to tetracyclines, penicillin and quinolones and their use in treating gonorrhoea has long since been discontinued Thus, Global action plan to control the spread and impact of antimicrobial...

Báo cáo khoa học: Ibuprofen binding to secondary sites allosterically modulates the spectroscopic and catalytic properties of human serum heme–albumin doc

... according to Eqn (7) [20]: koff ẳ koff2ị ibuprofen= K2 ibuprofen ỵ koff3ị ibuprofen= K3 ibuprofen ị ỵ kỵ off 7ị where koff(2) and koff(3) indicate values of koff occurring at K2 < [ibuprofen] ... 10)1 s)1) Ibuprofen binding to tHSAheme-Fe(II)-NO Figure shows the binding isotherm for ibuprofen binding to tHSAheme-Fe(II)-NO Analysis of the dependence of the molar fraction of the ibuprofenbound ... unequivocally, for the rst time, that ibuprofen allosterically modulates the spectroscopic and reactivity properties of tHSAheme and HSAheme by binding to the low-afnity secondary sites FA2 and FA6 rather...

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