... 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory anti-alpha5 and anti-beta1 monoclonal antibodies Evidence that the synergy sequence of fibronectin is ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... colony-forming cell and the haemopoietic stem cell Blood Cells (12):7-25 32 Moore KA, Lemischka IR 2006 ;Stem cellsand their niches Science (5769):1880-1885 33 Lemischka IR, Moore KA 2003 ;Stem cells: interactive...
... brain (neuronal stem cells) , skin (epidermal stem cells) , eye (retinal stem cells) and gut (intestinal crypt stem cells) (Spradling et al 2001) However, 15 not all organs and tissues may contain ... counterpart of murine EC cells for studying human development and differentiation invitro (Andrews 2002) hEC cell lines are capable of multi-lineage differentiation invitro but being of tumor origin ... classes of pluripotent stemcellsin humans, other primates and mice These are embryonicstem cells, embryonic germ cells, embryonic carcinoma cellsand recently the discovery of a fourth class of...
... could maintain a small accessible region in the globin locus during the differentiation of hematopoietic stemcells to erythroid cells Transcription of HS3 could be important in maintaining this ... differentiation of murine embryonicstemcells PCR analysis of DNase I treated and reverse-transcribed total RNA extracted from differentiating embryonicstemcells at the indicated time points All ... nature and extent of covalent histone modifications and association of transcription complexes at the murine b-globin locus during the invitro differentiation of murine embryonicstemcells We observed...
... expression profiles of miRNAs and transcripts using three hES cell lines, embryonic bodies (EB) produced from one of the cell lines and five types of terminally differentiated adult cells The integration ... expression inembryonicstemcells suggest they are involved in maintaining "stemness" Identifying mRNAs that are directly targeted by a specific miRNA is a major obstacle in understanding the miRNA ... greater in hES cells than in EB cellsand it was not even detected in adult cells The expression of miR-520b was 26-fold greater in hES cells then in EB cells (P < 0.05) and it was detected only in...
... methylcytosine cells, chromatin of pluripotent cells appears to have unique features, such as the increased mobility of many structural chromatin proteins, including histones and heterochromatin protein ... Figure The combination ofstem cell models and epigenomics in studies of the role of non-coding mutations in human disease Epigenomic analyses ofcells derived through invitrostem cell differentiation ... importance of epigenetic patterns in defining cell identity, understanding and utilizing epigenomic mapping will become a necessity in both basic and translational stem cell research In this review,...
... Foundation, and Parkinson's Action Network, as well as the Cancer Research and Prevention Foundation This type of federal research funding strategy involving existing embryonicstem cell lines is ... were obtained for further analysis Refinement and increased efficiency of these two ESC protocols brings the potential to offer a reliable supply ofembryonicstemcells without production of whole ... The end of the beginning for pluripotent stemcells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonicstem cell research: a Catholic viewpoint Kennedy Inst Ethics...
... regenerative medicine, I investigated the ability of two cell lines– hES2 and hES3– to generate derivatives of mesoderm and endoderm invitro Differentiation into these particular lineages was of interest ... studies detailing the derivationandmaintenanceof induced pluripotent stem (iPS) cells (Takahashi et al 2007; Takahashi and Yamanaka 2006) iPS cells are generated from mouse and human adult ... manufacturing practice) guidelines Even with derivationof qualified lines and defined culture methods, the recurring challenges of directing the differentiation of hESCs to generate cell types in numbers...
... pluripotency in human ES cells LIN28, an RNA binding protein, contains three RNA binding domains: one coldshock domain at the N terminus and two CCHC-type zinc finger domains at the C terminus It ... WNT wingless-type MMTV integration site family Page X Chapter Introduction 1.1 Human embryonicstemcells 1.1.1 Overview and characteristics of human embryonicstemcellsEmbryonicstem (ES) cells ... differentiation (Wu and Belasco, 2005) In C elegans, Lin4 binds imperfectly to complementary sites in the 3'-UTR and inhibits translation of target mRNAs, including Lin28, Hbl-1, and Lin14 (Lin et al.,...
... providing countless days of humor and inspiration, and providing invaluable thoughts on science and life, and all things in between Table of Contents Abstract Introduction Results Culturing of Mouse ... GFAP and Vimentin expressions in SDIA cultures List of Illustrations Illustration – Invitrogen BlockIT tetracycline inducible short, hairpin RNA system Introduction Mouse embryonicstemcells ... Blasticidin and (B) Zeocin that E14 and AB2.2 could not survive in µg/mL Blasticidin concentration, while E14 did not survive in 250 µg/mL Zeocin selection and AB2.2 in any concentration of Zeocin...
... alterations 128 trained in procedures and protocols designed to minimize low temperature exposure This would include quick handling outside the incubator, as well as the use of pre-warmed and ... pre-warmed and pre-equilibrated culture media [15, 16] Indeed, minimizing low temperature exposure of human embryos and oocytes is a critical factor in determining the success of clinical assisted reproduction ... layer of mitomycin C-inactivated murine embryonic fibroblast feeder (MEF) cells [7, 8] These were harvested from CF1 inbred mouse strain purchased from Charles River Laboratories Inc (Wilmington,...
... all-alpha domain, STAT protein DNA-binding domain and SH2 domain of stat6, and the zinc-finger domain, leucine-zipper domain and fork-head domain of foxp3 In addition, for stat6 and foxp3, there ... implicated in other protein–protein interactions, a STAT protein DNA-binding domain and an SH2 domain, which binds phosphorylated tyrosine residues in the context of a longer peptide motif within a ... binding and nuclear localization), which is shared by all FOX proteins, a leucine zipper domain and a C2H2 zinc finger domain, both of which are thought to help mediate DNA binding and may be involved...
... N-acetylornithine deacetylase; OCT, ornithine carbamoyltransferase; ASS, argininosuccinate synthase and ASL, argininosuccinate lyase by arginine [16,17] As a result, the concentration of citrulline and ... mitochondria [15,30,31], and ornithine is exported to the cytosol to be converted into citrulline and arginine [31,32] In plants, information on the localization of citrulline and arginine biosynthetic ... owing to its high thermostability and insensitivity to the inhibition induced by citrulline and arginine However, many different enzymes are involved in the metabolism of citrulline (Fig 1), and...
... expression of the 14-kDa phosphohistidine phosphatase and an endosomal protein may be of interest in the light of the recently described histidine phosphorylation of annexin I from a membrane preparation ... worth noting that annexin I shows endosomal binding in live HeLa cells [35] In conclusion, the native substrates of the 14-kDa phosphohistidine phosphatase described in the present work remain to ... glycoprotein from developing rat intestinal epithelial cells J Biol Chem 270, 1583–1588 33 Wilson, J.M & Colton, T (1997) Targeting of an intestinal apical endosomal protein to endosomes in nonpolarized...
... optimal imidazole concentrations needed to remove contaminating proteins in the washing step and to elute the recombinant enzyme in the elution step were determined in preliminary experiments Fractions ... 7.0 and were run at Expression in yeast and purification of recombinant BNSEH1 The BNSEH1 was expressed intracellularly in yeast cells at high levels ( £ 10% of the total protein and routinely ... laurate [20] and up-regulated palmitate, stearate and c-linolenate (x-6) B napus lines [21] Several genes encoding sEH exist in potato, soybean and Arabidopsis [11,13,22] and apparently also in...
... Breeding Laboratories, Inc., Wilmington, MA) were kept in sterile micro-isolator cages and fed food and water ad libitum The institutional guidelines of Johns Hopkins University concerning the ... flasks (Corning Flask 3056, Corning Inc., Corning NY) Cells used for experiments were low passage and maintained in culture for one to three months The doubling time of the cell line was measured ... was extracted and purified from cell lysates of 1-5 × 10 invitro FNAR tumor cellsand the REH cell line of normal rat endothelial cells, as a control, with 500 μl Trizol reagent (Invitrogen, Carlsbad,...
... stained positively (arrow) (e) Intense staining of individual cellsin the lining layer of a typical OA synovium (f) An area of an OA synovium demonstrates a lining layer completely devoid of ... c19orf10 staining (g) Intense staining of a hyperplastic RA synovial lining cell layer This staining was typical of most areas of RA synovium where the lining was hyperplastic (h) An area of an RA ... time -of- flight mass spectrometer [10], and proteins were identified by single MS (peptide mass fingerprinting) using ProFound [11] and by tandem mass spectrometry (MS/ MS) using Tandem search engine...
... the differentiation ofstemcells into the osteogenic lineage invitro J Bone Miner Res 2004, 19:1379-1394 Page of 38 Trounson A: Human embryonicstem cells: mother of all cell and tissue types ... prominent in the centre of the sphere, as demonstrated in histological sections stained with Figure Micromasses consisting ofembryonicstemcells were cultured with or without DAG and stained ... effect of fibronectin and laminin on cell retention and rate of osteogenic expression Cell Transplant 1992, 1:23-32 24 Meyer U, Meyer T, Jones DB: Attachment kinetics, proliferation rates and vinculin...
... of the growing polymer chain Upon transfer of the active site to another polymer chain, branching sites can arise causing a change in the morphology of the final polymer On the other hand, if the ... having to with the relative stabilities of the cyclic monomer (ring-strain) and linear polymer structure Typical initiation in ROP occurs via cationic or anionic means by the use of ionic initiators ... numbers of other synthetic polymers were developed and commercialized in response to the growing need for new materials in the automotive and aerospace industries Some of these new materials include...