In vitro study of surface functionalization of titanium substrates for potential enhancement of osseointegration and reduction of bacterial infection

... IN VITRO STUDY OF SURFACE FUNCTIONALIZATION OF TITANIUM SUBSTRATES FOR POTENTIAL ENHANCEMENT OF OSSEOINTEGRATION AND REDUCTION OF BACTERIAL INFECTION HU XUEFENG (B.Eng., ... 2007).Therefore, new strategies of surface functionalization of titanium implants have been investigated with the aim of improving osseointegration 2.4.1 Enhancement of osseointegration by surface ... 2.4.1 Enhancement of osseointegration by surface topography 15 2.4.2 Enhancement of osseointegration by surface chemistry 16 2.5 Surface modification of Ti to reduce infections 22 2.5.1 Surface...

báo cáo khoa học: " In vitro behaviour of endothelial cells on a titanium surface" doc

... successful clinical application Noteworthy, the evaluation of the interaction of cells and implanted materials must take into account other parameters in addition to the analysis of maintenance of cell ... (Takara Biomedicals, Japan); anti-human vitronectin, anti-human vitronectin receptor and anti-human VE-cadherin (Chemicon International, USA); anti-human αsmooth muscle actin (ICN Biomedicals, ... coverage of an EC layer was obtained without any coating or surface treatment Figureof days and (C) 14 days and to control integrin expression on endothelial cells attached to titanium after (A) ...

Báo cáo y học: "Characterization of Human Erythrocytes as Potential Carrier for Pravastatin: An In Vitro Study"

... of pravastatin in human erythrocytes The current work studies effect of time, temperature as well as drug concentration on the process of pravastatin loading into human erythrocytes by endocytosis ... characterization of pravastatin loaded erythrocytes Hematological Indices To determine the effect of loading process on erythrocytes, normal erythrocytes, erythrocytes suspended in PBS, and pravastatin-loaded ... Koitabashi Y, Kumai T, Matsumoto N, Watanabe M, Sekine S, Yanagida Y, Kobayashi S Orange juice increased the bioavailability of pravastatin, 3-hydroxy-3-methylglutaryl CoA reductase inhibitor, in...

báo cáo hóa học:" Fibrin and poly(lactic-co-glycolic acid) hybrid scaffold promotes early chondrogenesis of articular chondrocytes: an in vitro study" docx

... type II and aggrecan core protein was steadily expressed in fibrin/ PLGA and PLGA Interestingly, suppression of collagen type I was observed in fibrin/ PLGA and PLGA at weeks and weeks β-actin gene ... that fibrin would be an ideal cell carrier/transplantation matrix and to enhance in vitro chondrogenesis of rabbit articular chondrocytes by mean of morphological, histological, biochemical and ... filling up several void spaces of the scaffold For fibrin/ PLGA hybrid construct, accumulation of proteoglycan-rich matrix and GAG at the core region was significant and was intensely stained...

báo cáo hóa học:" The effect of newer anti-rheumatic drugs on osteogenic cell proliferation: an in-vitro study" doc

... light of these many uncertainties, we carried out an invitro study of the effect of various antirheumatoid drugs alone and in combination on the proliferation of osteogenic cells derived from the ... proliferation In addition, we found that the effects of these drugs on cell proliferation can vary between donors Although on average Dexamethasone increased cell proliferation, its effect varied strongly ... negate the influence of one anti-rheumatic drug (Dexamethasone) and increase the effect of another (Leflunomide) on cell proliferation Again, these interactions could differ between donors Averaged...

Báo cáo khoa học: "Contribution of the xenograft bone plate-screw system in lumbar transpedicular stabilization of dogs: an in-vitro study" docx

... Contribution of a xenograft bone plate-screw system in dogs 195 Discussion Fig The comparison of stiffness for xenograft bone platescrew following biomechanical test of the data, the software (Statistica'99; ... [1], the usability of bone as a spinal implant or instrument in the spinal surgery is possible Compare to the group I average stiffness values, the use of the XBPS system in group lV played an ... consensus about the reliability and clinical application of these systems [6] In addition, there have been no answers with the detailed information on which instrument is the best choice in spinal surgery...

Báo cáo y học: "An in vitro study investigating the survival and phenotype of mesenchymal stem cells following injection into nucleus pulposus tissue" pot

... within a small number of cells in monolayer culture Following injection into NP tissue explants, MSCs stained strongly positive for type II collagen and matrix staining in the vicinity of the injected ... viability/metabolic activity of injected mesenchymal stem cells Photomicrographs representative of caspase immunopositivity and polyAcells mRNA staining in mesenchymal stem cells injected into tissue ... vicinity of the injected cells was also observed week following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen...

Báo cáo y học: "Differential direct effects of cyclo-oxygenase-1/2 inhibition on proteoglycan turnover of human osteoarthritic cartilage: an in vitro study" pptx

... be consistent in vitro and in vivo The direct negative effects of indomethacin are mainly reflected by an inhibition of proteoglycan synthesis and diminished retention of these newly formed proteoglycans ... proteoglycan turnover (Table 1): low proteoglycan synthesis, high proteoglycan release (both newly formed proteoglycans and resident proteoglycans) and diminished proteoglycan content Baseline data ... ibuprofen, cause anti-inflammatory effects independent of COX activity and prostaglandin synthesis inhibition [45-47] These effects are mediated through inhibition of certain transcription factors...

Báo cáo y học: "Key regulatory molecules of cartilage destruction in rheumatoid arthritis: an in vitro study" docx

... secretion of soluble mediators by RASF with the use of custom antibody-based cytokine membrane arrays A proteomic analysis of these supernatants revealed an increased secretion of cytokines/ Available ... from RASF into the supernatant (RASFsn) and induced the catabolic and inflammatory response of chondrocytes after stimulation Protein analysis of the supernatant of RASF was used to analyze the ... trypsin and 0.02% EDTA (Biochrom) and cryopreserved After cryopreservation, human chondrocytes were expanded in a monolayer and, after reaching subconfluence again, the cells were trypsinized and...

Báo cáo y học: "Early Effects of anti-inflammatory [1, 2, 4]triazolo[4, 3-a] [1, 8]naphthyridine derivatives on human stimulated PMN and endothelial cells: an in vitro study" doc

... than the inhibition of cyclooxigenase activity, typical of nonsteroidal anti-inflammatory drugs (NSAIDs), and thus devoid of irritant effects on the gastric mucosa and suitable for use in chronic ... stimulus minus basal adhesion, and b is adhesion elicited by stimulus minus basal adhesion Prostacyclin and Prostaglandin E2 enzyme immunoassay The effect of IL-1α on the release of PGE2 and the ... non-linear regression model using the software Origin version 6.0 (Microcal Software, Northampton, USA) IC50 data in Figures and were analyzed using one-way analysis of variance, followed by...

In vitro and in vivo study of ABT 869 in treatment acute myeloid leukemia (AML) alone or in combination with chemotherapy or HDAC inhibitors insight into molecular mechanism and biologic characterization

... In vitro and In vivo study of ABT- 869 in treatment acute myeloid leukemia (AML) alone or in combination with chemotherapy or HDAC inhibitors: insight into molecular mechanism and biologic characterization ... target of STAT3 3.3.9 In vivo efficacy of IDR E804 in combination with ABT- 869 for treatment of MV4-11-R mouse xenografts 69 Discussion 73 References 78 Chapter The combination of HDAC Inhibitors and ... CalcuSyn software for (A) simultaneous combination of ABT- 869 with Ara-C, (B) simultaneous combination of ABT- 869 and Dox , (C) pretreatment with ABT- 869 first followed by Ara-C, (D) pretreatment with...

In vitro and in vivo study of vitamin e TPGs coated immunoliposomes for sustained and targeted delivery of docetaxel

... the herceptin conjugated liposomes Thus the herceptin conjugated Vitamin E TPGS coated liposomes showed greater potential for sustained and targeted chemotherapy in the treatment of HER2 over expressing ... have been discovered and developed since the past few decades Together with the problems faced in traditional ways in which cancer patients are treated, this regimen has even become more and ... formulations were provided Then, Chapter presents the preparation and characterization vitamin E TPGS coated and herceptin conjugated liposomes The liposomes were prepared by solvent injection method and...

In vivo and in vitro study on potential combination therapy of COL 3 and tamoxifen in breast cancer a pilot study

... that acute doxorubicin administration decreased COL- 3 oral bioavailability and prolonged COL- 3 elimination in rats[1 23] In the present study, a potential combination therapy of COL- 3 and tamoxifen ... combination of tamoxifen and COL- 3 is rational, based on the marked antitumor activity of both agents against a variety of solid tumors, such as breast cancer, prostate cancer, lung cancer, colon cancer, ... effects of COL- 3 and tamoxifen, in single agent or in combination against human breast cancer cell lines, MDA-MB- 231 and MCF-7 Finally, attempt would be made to account for an irregular absorption...

Analysis, sequencing and in vitro expression of PCR products

... numbers of samples are involved (Section 5.4) Analysis, sequencing and in vitro expression of PCR products 93 5.4 Direct DNA sequencing of PCR products Once a PCR product has been cloned into a ... primer in excess continues to allow linear accumulation of one strand of DNA suitable for subsequent DNA sequencing Analysis, sequencing and in vitro expression of PCR products 99 Template DNA PCR ... radioactive Analysis, sequencing and in vitro expression of PCR products 101 or ﬂuorescent detection A standard procedure is used for all templates in cycle sequencing and is superior to other PCR sequencing...

Báo cáo khoa học: In vitro expansion of DNA triplet repeats with bulge binders and different DNA polymerases pdf

... of the triangular prism binding pocket, with its amino sugar anchored Expansion of DNA repeat sequences in the major groove of the DNA pointing toward the 3¢ -bulge- ﬂanking base pair We were interested ... as the ligand–nucleic acid complex is formed To gain insight into the binding of drugs to bulge DNA, several bulgecontaining oligonucleotides were selected as binding hosts for DDI-1A and DDI-1B ... Effect of DDI-1A and DDI-1B on repeats expansion DDI-1A and DDI-1B were tested for their effect on the expansion of several doublet and triplet repeats in the presence of the Klenow fragment of DNA...

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