... H, Hasegawa M, Sugai S, Obata T, Ugi S, Morino K, Egawa K,Fujita T, Sakamoto T, Nishio Y, Kojima H, Haneda M, Yasuda H, Kikkawa R,Kashiwagi A: Expression of a dominant negative SHP-2 in transgenicmice ... its phospha-tase activity. In addition to its phosphatase activity,SHP-2 appears to function as a molecular adaptor asshown by Ali et al’sreportofaSHP-2IRScomplex[7]as well as its adaptor function ... ortho-vanadate and protease inhibitor cocktail, precleared withprotein A- Sepharose, and anti-SHP-2 or anti PLCb1anti-bodies bound to protein A- Sepharose were added at 4°C. This was then incubated...