báo cáo khoa học: " Identification of novel maize miRNAs by measuring the precision of precursor processing" ppsx

... 23 of the 66 new miRNAs. The targets of two of these newly identified miRNAs wereconfirmed by 5’RACE.Conclusion: We have implemented a novel method of identifying miRNA by measuring the precision ... component of the transcriptome. Science 2005,309(5740):1567-1569.doi:10.1186/1471-2229-11-141Cite this article as: Jiao et al.: Identification of novel maize miRNAs by measuring the precision of precursor ... 11:141http://www.biomedcentral.com/1471-2229/11/141Page 6 of 14RESEARCH ARTICLE Open Access Identification of novel maize miRNAs by measuring the precision of precursor processingYinping Jiao†, Weibin...

Tài liệu Báo cáo khoa học: Erythrochelin – a hydroxamate-type siderophore predicted from the genome of Saccharopolyspora erythraea docx

... in the etc gene cluster. The N-terminal C-domain of the NRPS catalyzes the a-N-acetylation of haOrn1in cis.Cyclorelease of the assembled tetrapeptide mediated by the C-terminal C-domain of ... characterization of these compounds isgrowing and may result in the synthesis of speciﬁcinhibitors based on the structure of the pathogensiderophore [16].A promising approach for the isolation of secondarymetabolites, ... derivatization-basedelucidation of the overall stereochemistry. Further-more, the functional properties of erythrochelin actingas an iron-chelating compound were investigated. On the basis of the analysis of the S....

Tài liệu Báo cáo khoa học: An immunomodulator used to protect young in the pouch of the Tammar wallaby, Macropus eugenii pptx

... (i.e. when the young has no immune system of its own), whenthere is a profound fall in the microbial ﬂora of the pouch [6]. Neither eugenin nor other low molecularmass components of the pouch ... asstimulation of the proliferation of lymphocytes, activa-tion of CCK receptors stimulate production of inter-leukins and secretion of immunoglobulins on the mucosal surface of the intestine [49,50]. The ... under-neath the spectrum) provide sequencing data from the N-terminal end of the peptide. The positive ion massspectrum (Fig. 3) provides the majority of the sequenceexcept that it does not identify the...

Tài liệu Báo cáo khoa học: Control analysis as a tool to understand the formation of the las operon in Lactococcus lactis doc

... the data fit well with the indi-vidual determination of flux control coefficients by PFK, PK and LDH. The role of the las operon is dis-cussed on the basis of the distribution of flux controlfor ... same operon; the cellcan then respond quickly to changes in the environ-ment by changing the expression of only a few genesand using the protein-synthesizing capacity to expressthese genes ... analysis of the las operonFEBS Journal 272 (2005) 2292–2303 ª 2005 FEBS 2293minute. The relative values of simultaneous modulation of the three las enzymes are calculated as the average of the three...

Tài liệu Báo cáo khoa học: Long-distance interactions between enhancers and promoters The case of the Abd-B domain of the Drosophila bithorax complex pdf

... perhaps reﬂects the differencebetween the complexities of the regulation of the twosystems: a longer time is required for the formation of the complex looping structure in the case of BX-C,while ... approach theirproper target promoter in the viscous environment of the nucleus.Somatic pairing of chromosomes and the regulation of Abd-BA peculiarity of Drosophila is the fact that the homo-logous ... protect a reporter gene from the effects of the neighboring chromatin (e.g. heterochro-matinization) if the reporter is ﬂanked by two of them. The apparent function of the boundaries within BX-Cis...

Báo cáo khoa học: Cyclic ADP-ribose requires CD38 to regulate the release of ATP in visceral smooth muscle ppt

... not the release of ATP evoked by action potential ﬁrings. The enhancing effect of cADPR on spontaneous release of ATP was: (a) unaf-fected by inhibition of P2 purinoreceptors; (b) abol-ished by ... change the release of ATP evoked by action potential ﬁring. These differential effects of cAD-PR can be explained by differences in the dependence of ‘spontaneous’ and ‘evoked’ release of neurotransmit-ters ... determine the mechanisms of purine-mediated presynaptic neuromod-ulation in the bladder. The enhancing effect of cADPR on the spontaneousrelease of ATP is not caused by activation of membrane-bound...

Báo cáo khoa học: Nuclear actin and actin-binding proteins in the regulation of transcription and gene expression docx

... polymerases, and the solution of the crystal structure of Pol II shows that these twosubunits are located close to each other at the surface of the polymerase [29,124] and participate in the tran-scription ... areas of the cell via its N-ter-minal ABD. In the absence of ligand, AR is localizedpredominantly in the cytoplasm, and its hinge domainand the LBD are tethered to the C-terminal end of FLNa ... liganded ARto the nucleus. In the nucleus, FLNa(16–24) disruptsinteractions between the N- and C-termini of the AR,and interferes with the binding of the coactivator TIF2[86,91]. There is evidence...

Báo cáo khoa học: Mycobacterium tuberculosis possesses a functional enzyme for the synthesis of vitamin C, L-gulono-1,4-lactone dehydrogenase doc

... attenuated virulence of the Candida albicansmutant [42]. If synthesized by the Rv1771 l-gulono-1,4-lactone dehydrogenase, L-AA may represent a novel weapon in the antioxidative arsenal of the pathogen ... the fusion protein wasdegraded by the host proteases. On the other hand,attempts to produce a His-tagged version of the Rv1771 protein by using the pRSETc or the GatewaypDEST17 expression vectors ... the intracellular growth of the pathogen in itshost. This could explain the absence of the l-gulono-1,4-lactone dehydrogenase reaction product in the M. tuberculosis cells grown in vitro.The...

Báo cáo khoa học: A simple in vivo assay for measuring the efﬁciency of gene length-dependent processes in yeast mRNA biogenesis doc

... of the THO com-plex for the expression of long genes [14], the GLAMratios of the two THO mutants were dramaticallyreduced when compared with the wild type (Fig. 6). The absence of effect of ... several plasmids containing the PHO5coding region transcribed under the control of the GAL1 promoter, but differing in the length of their3¢-UTR. To increase the length of the 3¢-UTR weinserted ... units,regardless of their length. In order to quantify the results of the assay, we have deﬁned gene length-dependent efﬁciency of mRNA accumulation as the levels of a long mRNA encoding PHO5, divided by the...

Báo cáo khoa học: Examining multiprotein signaling complexes from all angles The use of complementary techniques to characterize complex formation at the adapter protein, linker for activation of T cells pdf

... [18,22,23]. The interaction of phosphorylated LAT with these mole-cules was mediated by the central SH2 domains of Grb2 and Grap. The SH2 domain of both of these pro-teins is flanked by two SH3 ... the compositionand stoichiometry of these signaling complexes? Whatis the molecular mechanism for the induction of thesecomplexes? How does the formation of signaling com-plexes lead to the ... this review is to show the benefits of a comprehensive examination of the for-mation of signaling complexes by multiple techniques.Our example is the characterization of the hematopoi-etic-specific...

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