Báo cáo y học: "Effect of dsDNA binding to SmD-derived peptides on clinical accuracy in the diagnosis of systemic lupus erythematosus" potx

... articleEffect of dsDNA binding to SmD-derived peptides on clinical accuracy in the diagnosis of systemic lupus erythematosusMichael Mahler1, Aderajew Waka2, F Hiepe3 and Marvin J Fritzler41Development ... which may result in the detection of anti- dsDNA antibodies in the SmD peptide ELISA [32]. In the present study we confirmed the dsDNA binding property of the SmD1 peptide. This finding was further ... from the second serum panelFigure 2 Binding of SmD peptides to dsDNABinding of SmD peptides to dsDNA. (a) SmD1, SmD3 and biotinylated control peptides (PM1-α and Ribosomal P) were serially diluted...

Báo cáo Y học: Nuclear proteins that bind to metal response element a (MREa) in the Wilson disease gene promoter are Ku autoantigens and the Ku-80 subunit is necessary for basal transcription of the WD gene ppt

... sequences of MREafunction in sequence-specific interaction with the HepG2nuclear proteins.Characterization and purification of MREa -binding factors To determine the molecular mass of the MREa -binding proteins, ... cysteine-rich,metal -binding proteins that function in the maintenance of metal homeostasis and detoxification by forming strongcomplexes with several types of metal ions [16,17]. Given the existence of MREs in the ... oligonucleotide containing the binding sitefor transcription factor Sp1 revealed the presence of nuclearfactors that bind specifically to MREa. Two MREa -binding proteins of 70 and 82 kDa were purified using...

Báo cáo y học: "Dipeptidyl peptidase IV activity and/or structure homologs: Contributing factors in the pathogenesis of rheumatoid arthritis" doc

... Once in the vicinity of the synovium, the DPPIV activity would be able to initiateamino-terminal degradation of the increased levels of pro-inflammatory neuropeptides, cytokines and chemokines ... finding in RA, although not consistentlyobserved in the synovial fluid (Table 1). Despite the inconsistency in reporting of differing DPPIV levels betweennon-inflammatory and inflammatory synovial ... other cytokine production by monocytes [68,118,119]. Similarly, the pro-inflammatory effects of NPY in concanavalin A-induced paw edema in rat was potentiated byco-application of a DPPIV inhibitor...

Báo cáo y học: " TGF β-induced cartilage repair is maintained but fibrosis is blocked in the presence of Smad7" pps

... damage by injection of interleukin-1 (IL-1) intomurine knee joints. After 2 days, we injected an adenovirusencoding TGFβ. On day 4, we measured proteoglycan (PG)synthesis and content. To examine ... On day 2, an aden-ovirus encoding active TGF-β was injected into the knee joint. The TGF-β overexpression boosted the incorporation of 35SO42- beyond the already elevated incorporation to ... single one of the synoviallining cells and thereby fully block the TGF-β-induced fibrosis.We have previously demonstrated that blocking TGF-β withAd-Smad7 in OA resulted in reduction of the synovial...

Báo cáo Y học: Human sprouty 4, a new ras antagonist on 5q31, interacts with the dual speciﬁcity kinase TESK1 potx

... the discovery of the sprouty proteinfamily as ras inhibitors, induced by ras itself, contributes to the seemingly ever increasing complexity of ras signalmodulating mechanisms. In view of the ... representation of hspry4 and TESK1 proteins, whichinteract in yeast two-hybrid assay. The hspry4 protein, fused to the GAL4 DNA binding domain (BD), contains three potentially SH3 binding sequences ... activity of TESK1 is enhanced by ﬁbronectin-mediated integrin sign-aling, leading to phosphorylation of actin -binding coﬁlinand actin reorganization [17], and, as shown in this paper, the interaction...

Báo cáo y học: "β Treatment with recombinant interferon-β reduces inflammation and slows cartilage destruction in the collagen-induced arthritis model of rheumatoid arthritis" ppt

... hyperplasia of the intimal lining layer and cell infiltration of the synovial sublining weredetected and a decrease in bone erosions was observed. (b) Infiltration of inflammatory cells of the joints ... shown).IFN-ββmodulates the cytokine profile in inflamedsynovial tissue. To provide more insight into the mechanism by which IFN-β therapy exerts its beneficial effects, we examinedcytokine expression at the ... whether IFN-β isinfluencing monocyte recruitment, survival, and/orretention in the synovial joint.Alternatively, IFN-β may inhibit inflammatory cell infiltrationindirectly, via suppression of...

Báo cáo y học: " Bronchoalveoloar lavage fluid cytokines and chemokines as markers and predictors for the outcome of interstitial lung disease in systemic sclerosis patients" pps

... allowing the detection of cytokines without any BALFmanipulation that could influence the stability of cytokines. Byusing this technique, we observed abnormalities in a broadrange of cytokines ... differentiated into lymphocytic,neutrophilic, eosinophilic, and mixed forms (combination of lymphocytosis and granulocytosis).Bioplex analysisCytokine concentrations adjusted according to the recoveryrate ... diseases to identify key cytokines specifically involved in the pathogenesis of SSc-related lung disease. Furthermore, in a cross-sectional study, the correlation of cytokine andchemokine levels...

Báo cáo hóa học: " Research Article Cross-Layer Resource Scheduling for Video Trafﬁc in the Downlink of OFDMA-Based Wireless 4G Networks" docx

... connection may borrow tokens from the bank. The priority of a connection in borrowing tokensfrom the bank is determined by the priority index (Pi), givenbyPi=Eiri. (1)By prioritizing in ... modeled by considering the eﬀect of intercell interference and intracell interference on the sector of interest in the central cell (denoted as sector 1 in BS 1). Forthis purpose, the interference ... given to the users with maximum queuing delays weighted bytheir instantaneous and average rates [8]. The associateddecision metrics in these schemes are based on the com-bination of the delay...

Báo cáo Y học: Effect of coenzymes and thyroid hormones on the dual activities of Xenopus cytosolic thyroid-hormone-binding protein (xCTBP) with aldehyde dehydrogenase activity potx

... retinal, kinetics of the inhibition of xCTBP/xALDH1 by T3was examined by variation of NAD+concentration within the reaction mixture. The Kmvalue, 9 lM, was independent of the concentration of ... for cytosolicretinoic acid and retinol binding proteins where it has beensuggested that these binding proteins may act, not only asbuffers or reservoirs of intracellular retinoids to maintainsignificant ... later confirmed by examining both the 3,3¢,5-triiodo-L-thyronine (T3) binding and the ALDHactivities of the recombinant xALDH1 [10]. The concen-trations of the 59-kDa xCTBP, investigated by photoaffin-ity-labeling...

Báo cáo y học: " Effect of Weight Reduction on Cardiovascular Risk Factors and CD34-positive Cells in Circulatio"

... measurements The analysis of the CD34 positive cells was per-formed by adopting the gating strategy defined by the International Society of Haematotherapy and Graft Engineering (ISHAGE) guidelines ... study resulted in the improvement of the number of CD34+ cells in circulation and the decrease of the values of cardiovascular risk factors. According to our study, the circulating progenitor ... effects on the metabo-lism and inflammation, contributing to the mainte-nance of energy homeostasis and the pathogenesis of obesity-related metabolic and inflammatory compli-cations [4]. Endothelial...

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