... calcium are deleterious for the transparency ofthe lens [43,44]. The mechanisms and componentsinvolved such as sensors, buffers and modulators formaintaining calcium homeostasis in the lens are ... treatedMQ-water (Millipore, Bedford, MA, USA) and driedbefore use. The buffer used was 10 mm Tris-Cl (pH 7.5)containing 20 mm KCl. The spectra were recorded in the correct spectrum mode of the instrument ... bA3-crystallins were determined using a Microcal VP-ITC (Mic-roCal Inc., Northampton, MA, USA). Freshly preparedbB2-crystallin was used at a concentration of 341 lm in10 mm Tris-Cl (pH 7.5) containing...