... before the start of the reaction. During the first 15 min of the reaction, four samples of 10 lL each,taken at various time points 3, 6, 9 and 12 min after the start of the reaction, were applied to ... hTK1.In the present work, the part of the phosphatedonor that is necessary for the dimer–tetramer transi-tion of native hTK1 purified from human lymphocyteswas identified. Further, the effect of the ... According to this model, the simul-taneous presence in the assay of the two forms willresult in the apparent negative co-operative behavior. To further elucidate this, the relationship between the oligomerization...