... tuberculin syringe wasinserted through the second intercostal space to the left of the sternum, into the left ventricle. When bloodente red the tip of the needle, 5 × 104viable cancer cells in ... CF-AM-labeled and after washing, they were added(5 × 104cells/well) to quadruplicate wells. Then, plateswere incubated for 30 min, in the case of B16M cells, orfor 60 min in the case of A375M cells, ... systemic inflamma-tion [26-29]. Next, we studied the role o f COX-2 in the regulation of murine B16 and human A375 melanomacell adhesion and proliferation in response to primarycultured murine...