... nm,comparable to the inhibition of the aminopeptidasecathepsin H (Ki¼ 30 nm ). In comparison with othercystatins, cystatin F is a rather slow binding inhibitorof the cathepsins, characterized ... observed to be a tight bindinginhibitor of cathepsins F, K, L, V, with Kivalues ran-ging from 0.17 to 0.35 nm. Surprisingly cathepsin S,despite being an endopeptidase, was inhibited by cysta-tin ... processregulating its inhibitory properties.Screening of proteases for their inhibition showedthat cystatin F is different from other cystatins, both in terms of specificity and strength of binding to the target...