... the mutagenesis codon underlined) and 5¢-TTAGCGTATTCTAAAAGATACAAATAATCCTGAATGATATAAAAAC-3¢ (reverse). The pET151 HP1287 plasmidwas amplified using PfuTurbo DNA polymerase and incu-bated with ... F47Y mutation was performed with QuikChangeSite-Directed Mutagenesis Kit (Stratagene, La Jolla, CA,USA). The primers used were: 5¢-TATATCATTCAGGATTATTTGTATCTTTTAGAATACGCTAAGGTG-3¢ (forward, ... Structural and mutational analysis of TenA protein(HP1287) from the Helicobacter pylori thiaminsalvage pathway – evidence of a different substratespecificityNicola Barison1,2, Laura Cendron1,2,...