... band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6). Fig 10. SDS – PAGE pattern of PI-17 proteins Mc. Standard ... University of Helsinki, Finland, (1998), pp. 4-7. 5. Hernandez, J.F., Gagnon, J., Chiche, L., Nguyen, T.M, Andrieu, J.P., Heitz, A., Trinh H.T., Pham T.T. Chau., and Le Nguyen D., V. Squash ... stop codon and Xho I restriction site was introduced on the 5’ end of the proposed synthetic trypsin inhibitor gene. The sequences of the forward and reversed primers are shown on fig 3 and of...