... -7–EYFP and copine-2–EYFP ⁄ EGFP and its different C2C2-constructs as indicated above each panel. Methacholine was added to the medium containing extracellular calcium, attime point 0 s, and the ... calcium-dependent intracellular signalling.Materials and methodsDNA constructsCoding sequences of mouse copines-2 and -6, and humancopines-1, -3 and –7, were derived from image clones:IMAGE:3985959, ... -6 and -7 show different calcium-dependent intracellular membranetranslocation and targetingPavel V. Perestenko, Amy M. Pooler*, Maryam Noorbakhshnia, Adrian Grayà, Charlotte Bauccio§and...