it uses different methods from mathematics and the sciences ethical reasoning is a type of practical reasoning which in particular concerns certain societal or life form goals such as justice equality freedom health and safety
... 5'-AATACGACTCACTATAAGCAAAAGCAGG, complementary to the 3' end of viral RNA and 5'-CGGAATTCAATACGACTCACTATAAGTAGAAACAAGG for the 5' end The PCR conditions were 94° for 20 sec, anneal at 30° for ... passaging virus in normal MDCK cells without any exogenous sialidase There was no decrease in infectious titer during these passages and no change in HA activity Ferraris et al [28] also reported ... sequence, and 5'-CGGAATTCATTAACCCTCACTAAAAGTAGAAACAAGGAG for the 5' end The PCR conditions were 94° for min, then cycles of: 94° for min, anneal at 32° for min, extend at 72° for min, followed...
... and extradition.13 Informal assistance 13 For the purposes of this handbook, “informal assistance” is used to include any typeof assistance that does not require a formal MLA request Legislation ... on the merits and an imminent risk in delaying a decision The principal disadvantages of litigating ina foreign jurisdiction are the cost of tracing assets andthe legal fees entailed in obtaining ... expenses in restraining, maintaining, and disposing ofthe confiscated assets andthe legal and living expenses ofthe claimant Assets may also be returned directly to victims, including a foreign...
... Parade, fromwhich Allen's house is now reached by a passage way The house is built of stone, and has a very handsome front inthe style ofthe classical Renaissance In drawing aside the curtain, ... him A handsome specimen snuff box is now in Miss Freeling's hands Itis made of tortoise-shell, it has the portrait of King George the IVth asa gold medallion on the top, and was known asa Regency ... very happy to have assisted in giving such an accommodation to the city of Bath as he always hoped that plan would afford, andinwhich he is confirmed by the manner inwhichthe Corporation have...
... expressed inthe yeast Pichia pastoris, catalyzed the degradation ofa synthetic substrate containing a papain-cleavage site, as well as other proteins Moreover, a protein with strong proteolytic activity ... the His260 found in cathepsin B Asn280 (cathepsin B) is lacking in all expansins but is also absent fromthe C1 proteinase bromelain [26] andis not considered essential for catalysis in papain ... obtained from New England Biolabs, Beverly, MA, USA Pichia-transformation, identification of transformants, and expression Transformation of P pastoris strains GS115 and PEP4(thus proteinase A) -deficient...
... variability, ranging froma low of two up to a maximum of 218 recipes per brand, with an average of 34 The availability of nutritional information andthe recipe’s ease of preparation (or accessibility ... basic is to link the website to other brand-related advertising Internet advertising is readily integrated with other forms of media advertising and promotion At the most basic level, each form ... skittles.com visitors are asked for their favorite flavor, and then shown the poll results for each flavor In addition, they are encouraged to ask friends to vote for their favorite flavor and...
... features of lettered diagram and formulaic language are indeed a constant of Greek mathematicsas well asof its heirs inthe pre-modern Mediterranean ix x Preface mathematics, as formulated in ... was the mathematical point ofthe exercise? I assumed – asa generation of scholars was trained to assume – that Greek mathematics, certainly in its canonical form seen inthe works of Euclid, Apollonius, ... Arabic and Latin mathematics, as far asthe scientific revolution itself This form – ina nutshell, the combination ofthe lettered diagram with a formulaic language – isthe constant of Greek mathematics, ...
... intra-case and cross-case comparisons Intra-case comparison was used to contrast the reported experiences of individual Indian nurses at different stages of their migratory experience and adaptation ... Committee andfromthe Ethics Committee ofthe London School of Hygiene and Tropical Medicine Confidentiality and anonymity inthe process of data collection and analysis and reporting of findings was ... by andin turn affects multiple spheres ofthe migrant's life Three areas arising fromthe analysis comprised reasons for migration of an individual, social and cultural nature At the individual...
... Varghese V, Chaudhuri S, Barman P, Kojima K, Dutta P, Bhattacharya SK, Krishnan T, Kobayashi N, Naik TN: Genetic variability of human rotavirus strains isolated from Eastern and Northern India ... GIP performed the sequences analysis, phylogenetic analysis and wrote the manuscript; EEE download the sequences from database, performed the sequences analysis and critically revised the manuscript ... [7,12,17,20,21], andIn order to evaluate how many G1 strains included inthe GenBank database have these four specific mismatches and its clustering within specific lineages, we collected 173 sequences of the...
... molecular methods has led to an increase inthe importance ofmathematicsin population genetics and evolution The abundance of data that require mathematical analysis has greatly increased At the ... microorganisms There is also abundant evidence for increases in genetic complexity by the accumulation of small duplications And, as always, a lot of morphological and behavioral evolution is interesting ... population seemed a particularly unlikely place to find a new favorable gene combination, and this was indeed shown to be the case ina very detailed mathematical analysis by Barton and Charlesworth...
... make these inferences about the purity of GHN21 and GHN117, andthe other GHN strains [see Additional file 1], without the RIP analysis Thus, the polymerase genes of HIV-1 strains from Ghana are ... subtyping of all HIV-1 strains will be needed with resistance testing for patients failing therapy in countries with non-subtype B strains With the scale-up of antiretroviral therapy in Ghana, there ... were analyzed alone, itis likely that the evolution is towards that strain This may explain why fragments of CRF 02_AG strains [GeneBank: AJ286956 (5 in Additional file 1)] and [GeneBank: AJ583728...
... molecular analysis, andisin agreement with the findings of others for yeast, human renal and uterine myometrial tissues [15-17] The quality ofthe obtained RNA in our approach was feasible for ... we used the stability of 18S and 28S rRNA as markers for totalRNA and mRNA stability Histological evaluation was based on HE, reticulin, rhodanine and rubeanic acid stains and three different ... today Since biopsies fixed in either RNAlater or formalin remain stable at room temperature, transport is easy fromthe clinical situation to the research facility for further processing as well...
... restore the hydrological conditions inthe Taylor Slough and Eastern Panhandle basins, eliminate damaging freshwater flows to Manatee Bay and Barnes Sound in Biscayne National Park, and maintain flood ... Alberts is president ofthe National Academy ofSciencesThe National Academy of Engineering was established in 1964, under the charter ofthe National Academy of Sciences, asa parallel organization ... 2002 A summary ofthe panel's findings follows The National Academies consists ofthe National Academy of Sciences, the National Academy of Engineering, andthe Institute of Medicine The National...
... general, the increase inthe oxidation rate of crosslinked Hb mediated by LPSs is due to an increase inthe rate ofthe initial fast phase, i.e oxidation ofthea chains The rates of oxidation are ... which can then accelerate the displacement ofthe protonated superoxide anion, as was suggested by Tsuruga and Shikama [21] to explain the increase in oxidation rate ofthea chains inthe ab dimer ... times faster than that ofthe beta chains and that the oxidation ofthe beta chains was not in uenced by pH The biphasic reaction was shown to consist ofa rapid initial reaction followed by a slower...
... The assay was performed at mm carnitine as standard To analyze PigE17DCPT1B and HumanD17ECPT1B mutants, the assay was performed at carnitine concentrations equal to the Km The percentage of activity ... Roles ofthe N- and C-terminal domains of carnitine palmitoyltransferase I isoforms in malonyl-CoA sensitivity ofthe enzymes: insights from expression of chimaeric proteins and mutation of conserved ... VA & Price NT (2000) Identification of positive and negative determinants of malonylCoA sensitivity and carnitine affinity within the amino termini of rat liver- and muscle -type carnitine palmitoyltransferase...
... staining with ethidium bromide The intensities ofthe amplified fragments were quantified by densitometric analysis As an internal control 791 base pairs of a- actin was amplified using forward and ... proteins that bind specifically to MREa play an important role asa basal regulator of WD gene transcription MATERIALS ANDMETHODS Cell culture The human hepatoma cell line HepG2 was obtained from ... observed another band of 82 kDa when the MREa-protein band fromthe EMSA was excised fromthe native gel and resolved by SDS/PAGE (unpublished data) We purified the MREa-binding proteins using the avidin–biotin...
... data analysis methodsand their application in waste management research and practice is given Multivariate data analysis in waste management The main objectives of multivariate data analysis are ... Mahalanobis-Taguchi integrated with Artificial Neural Networks in data mining to look for patterns and modeling in manufacturing Pal and Maiti (2010) have adopted the Mahalanobis-Taguchi algorithm ... mathematically defined as an orthogonal linear transformation that arranges the data to a new coordinate system in that the greatest variance by any projection ofthe data takes place along the...
... (known for Scania), German city Herogenaurach (location for Adidas, Puma), andthe Aran Islands off the coast of Ireland famous for woollen sweaters (Kotler et al 1999) The Island of Harris off the ... essential, the branding aspect within place marketing will probably be one major tool for places, inthe same way as branding has been a major success dynamo for private corporations for many decades ... franchise 33 Financial World began to calculate the value ofthe Coca-Cola brand in 1993 with the worldwide sales for the Coca-Cola brand family at USD billion, andthe brand value was calculated...
... epitope on collagen This has significant implications for the use of saratin asa tool to inhibit platelet–collagen interactions, and may provide the basis for the therapeutic use of saratin as ... performed inthe absence (black bars) orthe presence (white bars) ofthe ADPremoving enzyme apyrase (apy) andthe cyclooxygenase inhibitor indomethacin (indo) as indicated The numbers of adherent ... its alimentary habit of hematophagy The presence of anticoagulants inthe salivary glands ofthe leech, Hirudo medicinalis, was originally discovered by Haycraft in 1884 and led to the isolation...