... in the absence of the other substrate. In the presence of oxoglutarate,however, coenzyme binding was tighter and thereforemeasurable and, in particular, gave measurable Kdvalues for binding ... requires further structuralinsight is, on the one hand, the widespread use of Aspat the P7 position in many naturally occurringNADPH-dependent GDHs and, on the other hand, the failure of the E243D ... evolution of nucleotide-binding protein.Nature 250, 194–199.5 Wierenga RK, Terpstra P & Hol WG (1986) Prediction of the occurrence of the ADP-binding bab-fold in pro-teins, using an amino acid...