... to the aforementioned C-terminal segment,including the androgen receptor [35], F-actin [36],14-3-3 [26] and the SPRY domain-containing suppressorof cytokine signalling box proteins 1, 2 and ... with other binding partners at neutral pH. Alterna-tively, interactions with other parts of the protein,including the N-terminal region of the coiled-coil (i.e.N-terminal to the leucine zipper), ... for5 min. Proteins were loaded on a 10% Tricine-PAGE gel[99]. The extent of digestion was measured from the relativeintensities of the Tricine-PAGE gel band representing the undigested band by...